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Top : New Forum Archives (2009-): : Protein Expression and Purification

631. Protein precipitates out so fast - (reply: 5)
632. Protein sticks to Ni beads - Protein doesn't elute from the column/batch etc (reply: 2)
633. Lysis Buffer for Total Protein Assay - (reply: 1)
634. primer design for pBAD topo expression kit - (reply: 2)
635. expression in minimal media - (reply: 14)
636. pQE vector protein expression - (reply: 3)
637. A story of Urea, dialysis and GST affinity purification... hopefully! - (reply: 1)
638. Which buffer to use? - (reply: 3)
639. Elimination of the polyethylenimine - (reply: 9)
640. subcellular fractionation problem - (reply: 1)
641. resuspend membrane pellet - (reply: 2)
642. problems with detecting protein expression - protein expression in cell (reply: 4)
643. Who know anything about dialysis on protein extractions ? - (reply: 7)
644. Protein doesn't bind any column except Ni - (reply: 10)
645. Kozak consensus sequence for insect expression - (reply: 1)
646. Sticky Insect cell lysate blocked Ni_NTA column - (reply: 2)
647. purification of a basic protein - problem in elution (reply: 4)
648. stabilization of purified protein solutions - (reply: 2)
649. Commercially available bacterial expression system with chaperone coexpression - (reply: 1)
650. Showed a bigger and broad smear of protein!! - (reply: 4)
651. Ptn expressed once... and never again!!! - (reply: 1)
652. collagen precipitation - (reply: 2)
653. SWAT for protein expression - problems with Poly Ala/GC Rich (reply: 1)
654. Mammalian protein expression in E. coli - (reply: 1)
655. Enzyme kinetics - quantification of reversible enzyme inhibition (reply: 1)
656. no expression of my protein - (reply: 1)
657. confirming nucleotide state of isolated G protein - (reply: 1)
658. pgex - (reply: 5)

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