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Top : New Forum Archives (2009-): : Protein Expression and Purification

631. B-per GST fusion protein purification kit - (reply: 2)
632. Protein precipitates out so fast - (reply: 5)
633. Protein sticks to Ni beads - Protein doesn't elute from the column/batch etc (reply: 2)
634. Lysis Buffer for Total Protein Assay - (reply: 1)
635. primer design for pBAD topo expression kit - (reply: 2)
636. expression in minimal media - (reply: 14)
637. pQE vector protein expression - (reply: 3)
638. A story of Urea, dialysis and GST affinity purification... hopefully! - (reply: 1)
639. Which buffer to use? - (reply: 3)
640. Elimination of the polyethylenimine - (reply: 9)
641. subcellular fractionation problem - (reply: 1)
642. resuspend membrane pellet - (reply: 2)
643. problems with detecting protein expression - protein expression in cell (reply: 4)
644. Who know anything about dialysis on protein extractions ? - (reply: 7)
645. Protein doesn't bind any column except Ni - (reply: 10)
646. Kozak consensus sequence for insect expression - (reply: 1)
647. Sticky Insect cell lysate blocked Ni_NTA column - (reply: 2)
648. purification of a basic protein - problem in elution (reply: 4)
649. stabilization of purified protein solutions - (reply: 2)
650. Commercially available bacterial expression system with chaperone coexpression - (reply: 1)
651. Showed a bigger and broad smear of protein!! - (reply: 4)
652. Ptn expressed once... and never again!!! - (reply: 1)
653. collagen precipitation - (reply: 2)
654. SWAT for protein expression - problems with Poly Ala/GC Rich (reply: 1)
655. Mammalian protein expression in E. coli - (reply: 1)
656. Enzyme kinetics - quantification of reversible enzyme inhibition (reply: 1)
657. no expression of my protein - (reply: 1)
658. confirming nucleotide state of isolated G protein - (reply: 1)
659. pgex - (reply: 5)

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