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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
631. truncation of protein expression - (reply: 1)
632. GST fusion protein purification - (reply: 1)
633. unstable protein expression - (reply: 1)
634. B-per GST fusion protein purification kit - (reply: 2)
635. Protein precipitates out so fast - (reply: 5)
636. Protein sticks to Ni beads - Protein doesn't elute from the column/batch etc (reply: 2)
637. Lysis Buffer for Total Protein Assay - (reply: 1)
638. primer design for pBAD topo expression kit - (reply: 2)
639. expression in minimal media - (reply: 14)
640. pQE vector protein expression - (reply: 3)
641. A story of Urea, dialysis and GST affinity purification... hopefully! - (reply: 1)
642. Which buffer to use? - (reply: 3)
643. Elimination of the polyethylenimine - (reply: 9)
644. subcellular fractionation problem - (reply: 1)
645. resuspend membrane pellet - (reply: 2)
646. problems with detecting protein expression - protein expression in cell (reply: 4)
647. Who know anything about dialysis on protein extractions ? - (reply: 7)
648. Protein doesn't bind any column except Ni - (reply: 10)
649. Kozak consensus sequence for insect expression - (reply: 1)
650. Sticky Insect cell lysate blocked Ni_NTA column - (reply: 2)
651. purification of a basic protein - problem in elution (reply: 4)
652. stabilization of purified protein solutions - (reply: 2)
653. Commercially available bacterial expression system with chaperone coexpression - (reply: 1)
654. Showed a bigger and broad smear of protein!! - (reply: 4)
655. Ptn expressed once... and never again!!! - (reply: 1)
656. collagen precipitation - (reply: 2)
657. SWAT for protein expression - problems with Poly Ala/GC Rich (reply: 1)
658. Mammalian protein expression in E. coli - (reply: 1)
659. Enzyme kinetics - quantification of reversible enzyme inhibition (reply: 1)
660. no expression of my protein - (reply: 1)

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