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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
511. affinity column problems - (reply: 1)
512. Desalting protein - (reply: 7)
513. Trypsin Expression and Purification - (reply: 3)
514. seeking help in stable cell line generation - (reply: 3)
515. BL 21 cells - (reply: 4)
516. Thiamine HCl 1M solution? - can I store it at RT? (reply: 2)
517. Low level of expression - in pASK-IBA7 vector (reply: 6)
518. dnase treatment before his-tag purification - (reply: 3)
519. protein aggregation problem - (reply: 3)
520. protein precipitates out during concentrating it - (reply: 2)
521. how to put in evidence protein after IP - (reply: 2)
522. GST-fusion protein purification - How to purify it? (reply: 9)
523. IPTG - (reply: 2)
524. Protein lysis buffer - (reply: 4)
525. IP low protein and MS - (reply: 1)
526. Inducible expression in human cell line - suggestions for a good system? (reply: 4)
527. Microcon troubles - Very low recovery (reply: 4)
528. Trouble with protein expression in E.coli - No expression of ~ 9-11 kDa protein (reply: 2)
529. Antibody-beads crosslink - troubles (reply: 5)
530. What is stringent vector? - Is it related to level of expression? (reply: 2)
531. Elution problens - My protein is not eluting in urea 8M buffer ... oh shitt!! (reply: 1)
532. expression vector in E. coli - the best expression vector in E. coli (reply: 5)
533. Concentrating Protein - protein precipitates upon concentrating (reply: 1)
534. Endogenous protein purification - (reply: 2)
535. Protein in and out of solution - (reply: 1)
536. Protein extraction from rat aorta - (reply: 3)
537. heterodimer - bicistronic vector? (reply: 2)
538. stripped Ni column turn brown yellow - (reply: 1)
539. Making Recombinant Proteins - (reply: 2)
540. GST- protein, batch purification - no protein after elution (reply: 3)

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