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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
511. Amylose bound protein elution - (reply: 1)
512. hey guys,what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
513. Soluble aggregates - (reply: 3)
514. insect protein expression - (reply: 1)
515. Can you store TNT translation products? - (reply: 1)
516. How to pack the FPLC column - (reply: 2)
517. Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
518. Sonication issues! - (reply: 3)
519. complete gene for expression? - (reply: 2)
520. buffer for membrane protein - (reply: 1)
521. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
522. Question: Bradford assay - buffer precipitates - KCl and HEPES preciptation with Bradford reagent (reply: 3)
523. membrane protein purification - (reply: 6)
524. Protein expression failing - (reply: 1)
525. How does aggregation influence absorbtion at 280nm? - (reply: 1)
526. Buttery Popcorn Odor in Contaminated Expression Prep - (reply: 3)
527. Self-made Protease inhibitor cocktail - PMSF, EDTA, Benzamidine (reply: 4)
528. Contamination in GST fusion protein purification - (reply: 2)
529. GST-tagged proteins - (reply: 1)
530. Protino® Ni-IDA Packed Columns - Can you re-use them? (reply: 1)
531. immunoprecipation - (reply: 1)
532. Ni-NTA his tag protein purification - (reply: 1)
533. Weird Induction Problem - (reply: 5)
534. cloning of non-coding region for protein expression - protein expression (reply: 1)
535. from pet-SUMO to pet-28 - Expression problems with pet SUMO (reply: 2)
536. Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
537. Protein expression - (reply: 4)
538. EDTA bacterial cell lysate - (reply: 2)
539. Trouble With HIS affinity column (Ni+) binding - (reply: 1)
540. Problems with affinity purification with M2 beads - (reply: 2)

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