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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
511. Problem with Protein elution using GST sepharose 4B - (reply: 1)
512. how to isolate insoluble protein in bacterial induction? - (reply: 4)
513. Plasmid instability in E.coli BL21? - (reply: 3)
514. Cleaving Fusion Proteins - (reply: 1)
515. Plyse cell and DH5a - (reply: 1)
516. Quality of aliquot for SDS-page in protein purification - When is pure pure enough? (reply: 1)
517. Electroelution with no electroeluter - (reply: 3)
518. reusing Ni NTA - (reply: 2)
519. Low expression: What else can I do? - Using pET23a in BL21 PlysS (reply: 9)
520. Changing dialysis buffer every half hour and then dialysis ON - protein precipitation prevention (reply: 1)
521. Checking For Protein Expression - (reply: 3)
522. How do I get rid of this contaminant? - His-tagged protein eluted with 300mM imidazole (reply: 13)
523. Is it possible? Harsh sonificiation conditions cause degradation of my protein - (reply: 3)
524. Low Thrombin Cleavage Efficiency Problem - I am trying to cleave a GST tagged protein(about 35 kDa + 26 kDa GST) (reply: 1)
525. Throbin cleavage declines with older protein? - It used to work, now doesn't! (reply: 1)
526. Couple questions about GST purification - (reply: 16)
527. Immunoprecipitation - (reply: 12)
528. Newbie question: how to determine protein concentration after IP? - (reply: 8)
529. protein extraction using trizol - can we replace guanidine cholride with other denaturants? (reply: 1)
530. High pressure on Ni-NTA beads - (reply: 2)
531. affinity column problems - (reply: 1)
532. Desalting protein - (reply: 7)
533. Trypsin Expression and Purification - (reply: 3)
534. seeking help in stable cell line generation - (reply: 3)
535. BL 21 cells - (reply: 4)
536. Thiamine HCl 1M solution? - can I store it at RT? (reply: 2)
537. Low level of expression - in pASK-IBA7 vector (reply: 6)
538. dnase treatment before his-tag purification - (reply: 3)
539. protein aggregation problem - (reply: 3)
540. protein precipitates out during concentrating it - (reply: 2)

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