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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
511. GST-fusion protein purification - How to purify it? (reply: 9)
512. IPTG - (reply: 2)
513. Protein lysis buffer - (reply: 4)
514. IP low protein and MS - (reply: 1)
515. Inducible expression in human cell line - suggestions for a good system? (reply: 4)
516. Microcon troubles - Very low recovery (reply: 4)
517. Trouble with protein expression in E.coli - No expression of ~ 9-11 kDa protein (reply: 2)
518. Antibody-beads crosslink - troubles (reply: 5)
519. What is stringent vector? - Is it related to level of expression? (reply: 2)
520. Elution problens - My protein is not eluting in urea 8M buffer ... oh shitt!! (reply: 1)
521. expression vector in E. coli - the best expression vector in E. coli (reply: 5)
522. Concentrating Protein - protein precipitates upon concentrating (reply: 1)
523. Endogenous protein purification - (reply: 2)
524. Protein in and out of solution - (reply: 1)
525. Protein extraction from rat aorta - (reply: 3)
526. heterodimer - bicistronic vector? (reply: 2)
527. stripped Ni column turn brown yellow - (reply: 1)
528. Making Recombinant Proteins - (reply: 2)
529. GST- protein, batch purification - no protein after elution (reply: 3)
530. Nickel IMAC - How to avoid unwanted proteins? - (reply: 4)
531. Insoluble TEV Protease - (reply: 2)
532. How to elute this protein?! - (reply: 5)
533. Recombinant protein or peptide? - (reply: 1)
534. Purifying nuclear fraction from skeletal muscle homogenate - (reply: 1)
535. pqe30 - (reply: 1)
536. truncation of protein expression - (reply: 1)
537. GST fusion protein purification - (reply: 1)
538. unstable protein expression - (reply: 1)
539. B-per GST fusion protein purification kit - (reply: 2)
540. Protein precipitates out so fast - (reply: 5)

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