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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
511. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
512. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
513. Protein precipitates upon dialysis - (reply: 5)
514. membrane protein aggregation - (reply: 1)
515. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
516. His Tag Protein Purification - (reply: 6)
517. Amylose bound protein elution - (reply: 1)
518. hey guys,what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
519. Soluble aggregates - (reply: 3)
520. insect protein expression - (reply: 1)
521. Can you store TNT translation products? - (reply: 1)
522. How to pack the FPLC column - (reply: 2)
523. Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
524. Sonication issues! - (reply: 3)
525. complete gene for expression? - (reply: 2)
526. buffer for membrane protein - (reply: 1)
527. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
528. Question: Bradford assay - buffer precipitates - KCl and HEPES preciptation with Bradford reagent (reply: 3)
529. membrane protein purification - (reply: 6)
530. Protein expression failing - (reply: 1)
531. How does aggregation influence absorbtion at 280nm? - (reply: 1)
532. Buttery Popcorn Odor in Contaminated Expression Prep - (reply: 3)
533. Self-made Protease inhibitor cocktail - PMSF, EDTA, Benzamidine (reply: 4)
534. Contamination in GST fusion protein purification - (reply: 2)
535. GST-tagged proteins - (reply: 1)
536. Protino® Ni-IDA Packed Columns - Can you re-use them? (reply: 1)
537. immunoprecipation - (reply: 1)
538. Ni-NTA his tag protein purification - (reply: 1)
539. Weird Induction Problem - (reply: 5)
540. cloning of non-coding region for protein expression - protein expression (reply: 1)

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