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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
511. Contamination in GST fusion protein purification - (reply: 2)
512. GST-tagged proteins - (reply: 1)
513. Protino® Ni-IDA Packed Columns - Can you re-use them? (reply: 1)
514. immunoprecipation - (reply: 1)
515. Ni-NTA his tag protein purification - (reply: 1)
516. Weird Induction Problem - (reply: 5)
517. cloning of non-coding region for protein expression - protein expression (reply: 1)
518. from pet-SUMO to pet-28 - Expression problems with pet SUMO (reply: 2)
519. Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
520. Protein expression - (reply: 4)
521. EDTA bacterial cell lysate - (reply: 2)
522. Trouble With HIS affinity column (Ni+) binding - (reply: 1)
523. Problems with affinity purification with M2 beads - (reply: 2)
524. Expression problem using invitrogen pRSET C vector - (reply: 5)
525. Vector containing N-his tag and TEV - (reply: 1)
526. Protein expression using invitrogen pSecTag2 - (reply: 2)
527. Problems with nuclear extraction - (reply: 1)
528. His-tagged protein runs much faster than expected - 6His-Tagged CcpA protein runs around 26 kD whereas I expect to run 37 (reply: 7)
529. Removing phosphates - (reply: 2)
530. protein going to inclusion bodies - protein going to inclusion bodies (reply: 1)
531. Looking for endogenus expression by using custom antibody - (reply: 5)
532. Adventitious chemical binding - Unwanted chemicals in protein solution (reply: 2)
533. More than one protein induced - (reply: 4)
534. Protein aggregates - Monomeric fraction varies with wavelength (reply: 1)
535. water withdrawal with PEG20.000 - Does it drag glycerol along too?-PLEASE HELP! (reply: 1)
536. Troubleshooting GST purification - (reply: 4)
537. Amount of inoculum? - for protein expression in E.coli (reply: 3)
538. BL21A or simliar - (reply: 2)
539. Role of imidazole in lysis buffer - for obtaining recombinant his-tag protein (reply: 4)
540. protein expression (temperature issue) - trying to express soluble protein at lower temperature (reply: 17)

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