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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
511. membrane protein purification - (reply: 6)
512. Protein expression failing - (reply: 1)
513. How does aggregation influence absorbtion at 280nm? - (reply: 1)
514. Buttery Popcorn Odor in Contaminated Expression Prep - (reply: 3)
515. Self-made Protease inhibitor cocktail - PMSF, EDTA, Benzamidine (reply: 4)
516. Contamination in GST fusion protein purification - (reply: 2)
517. GST-tagged proteins - (reply: 1)
518. Protino® Ni-IDA Packed Columns - Can you re-use them? (reply: 1)
519. immunoprecipation - (reply: 1)
520. Ni-NTA his tag protein purification - (reply: 1)
521. Weird Induction Problem - (reply: 5)
522. cloning of non-coding region for protein expression - protein expression (reply: 1)
523. from pet-SUMO to pet-28 - Expression problems with pet SUMO (reply: 2)
524. Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
525. Protein expression - (reply: 4)
526. EDTA bacterial cell lysate - (reply: 2)
527. Trouble With HIS affinity column (Ni+) binding - (reply: 1)
528. Problems with affinity purification with M2 beads - (reply: 2)
529. Expression problem using invitrogen pRSET C vector - (reply: 5)
530. Vector containing N-his tag and TEV - (reply: 1)
531. Protein expression using invitrogen pSecTag2 - (reply: 2)
532. Problems with nuclear extraction - (reply: 1)
533. His-tagged protein runs much faster than expected - 6His-Tagged CcpA protein runs around 26 kD whereas I expect to run 37 (reply: 7)
534. Removing phosphates - (reply: 2)
535. protein going to inclusion bodies - protein going to inclusion bodies (reply: 1)
536. Looking for endogenus expression by using custom antibody - (reply: 5)
537. Adventitious chemical binding - Unwanted chemicals in protein solution (reply: 2)
538. More than one protein induced - (reply: 4)
539. Protein aggregates - Monomeric fraction varies with wavelength (reply: 1)
540. water withdrawal with PEG20.000 - Does it drag glycerol along too?-PLEASE HELP! (reply: 1)

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