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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
511. buffer for membrane protein - (reply: 1)
512. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
513. Question: Bradford assay - buffer precipitates - KCl and HEPES preciptation with Bradford reagent (reply: 3)
514. membrane protein purification - (reply: 6)
515. Protein expression failing - (reply: 1)
516. How does aggregation influence absorbtion at 280nm? - (reply: 1)
517. Buttery Popcorn Odor in Contaminated Expression Prep - (reply: 3)
518. Self-made Protease inhibitor cocktail - PMSF, EDTA, Benzamidine (reply: 4)
519. Contamination in GST fusion protein purification - (reply: 2)
520. GST-tagged proteins - (reply: 1)
521. Protino® Ni-IDA Packed Columns - Can you re-use them? (reply: 1)
522. immunoprecipation - (reply: 1)
523. Ni-NTA his tag protein purification - (reply: 1)
524. Weird Induction Problem - (reply: 5)
525. cloning of non-coding region for protein expression - protein expression (reply: 1)
526. from pet-SUMO to pet-28 - Expression problems with pet SUMO (reply: 2)
527. Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
528. Protein expression - (reply: 4)
529. EDTA bacterial cell lysate - (reply: 2)
530. Trouble With HIS affinity column (Ni+) binding - (reply: 1)
531. Problems with affinity purification with M2 beads - (reply: 2)
532. Expression problem using invitrogen pRSET C vector - (reply: 5)
533. Vector containing N-his tag and TEV - (reply: 1)
534. Protein expression using invitrogen pSecTag2 - (reply: 2)
535. Problems with nuclear extraction - (reply: 1)
536. His-tagged protein runs much faster than expected - 6His-Tagged CcpA protein runs around 26 kD whereas I expect to run 37 (reply: 7)
537. Removing phosphates - (reply: 2)
538. protein going to inclusion bodies - protein going to inclusion bodies (reply: 1)
539. Looking for endogenus expression by using custom antibody - (reply: 5)
540. Adventitious chemical binding - Unwanted chemicals in protein solution (reply: 2)

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