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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
511. membrane protein extraction buffer - (reply: 1)
512. What about the Baculovirus expression system? - (reply: 2)
513. Ni-NTA beads and dialysis tubing - Purification using nickel beads (reply: 4)
514. Concentrating Protein with centrifuge concentrator but 50% glycerol!!? - (reply: 2)
515. protein is degraded after purification - (reply: 2)
516. protein expression - Preparation of starter cultures (reply: 15)
517. Problem with Protein elution using GST sepharose 4B - (reply: 1)
518. how to isolate insoluble protein in bacterial induction? - (reply: 4)
519. Plasmid instability in E.coli BL21? - (reply: 3)
520. Cleaving Fusion Proteins - (reply: 1)
521. Plyse cell and DH5a - (reply: 1)
522. Quality of aliquot for SDS-page in protein purification - When is pure pure enough? (reply: 1)
523. Electroelution with no electroeluter - (reply: 3)
524. reusing Ni NTA - (reply: 2)
525. Low expression: What else can I do? - Using pET23a in BL21 PlysS (reply: 9)
526. Changing dialysis buffer every half hour and then dialysis ON - protein precipitation prevention (reply: 1)
527. Checking For Protein Expression - (reply: 3)
528. How do I get rid of this contaminant? - His-tagged protein eluted with 300mM imidazole (reply: 13)
529. Is it possible? Harsh sonificiation conditions cause degradation of my protein - (reply: 3)
530. Low Thrombin Cleavage Efficiency Problem - I am trying to cleave a GST tagged protein(about 35 kDa + 26 kDa GST) (reply: 1)
531. Throbin cleavage declines with older protein? - It used to work, now doesn't! (reply: 1)
532. Couple questions about GST purification - (reply: 16)
533. Immunoprecipitation - (reply: 12)
534. Newbie question: how to determine protein concentration after IP? - (reply: 8)
535. protein extraction using trizol - can we replace guanidine cholride with other denaturants? (reply: 1)
536. High pressure on Ni-NTA beads - (reply: 2)
537. affinity column problems - (reply: 1)
538. Desalting protein - (reply: 7)
539. Trypsin Expression and Purification - (reply: 3)
540. seeking help in stable cell line generation - (reply: 3)

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