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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
511. hey guys,what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
512. Soluble aggregates - (reply: 3)
513. insect protein expression - (reply: 1)
514. Can you store TNT translation products? - (reply: 1)
515. How to pack the FPLC column - (reply: 2)
516. Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
517. Sonication issues! - (reply: 3)
518. complete gene for expression? - (reply: 2)
519. buffer for membrane protein - (reply: 1)
520. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
521. Question: Bradford assay - buffer precipitates - KCl and HEPES preciptation with Bradford reagent (reply: 3)
522. membrane protein purification - (reply: 6)
523. Protein expression failing - (reply: 1)
524. How does aggregation influence absorbtion at 280nm? - (reply: 1)
525. Buttery Popcorn Odor in Contaminated Expression Prep - (reply: 3)
526. Self-made Protease inhibitor cocktail - PMSF, EDTA, Benzamidine (reply: 4)
527. Contamination in GST fusion protein purification - (reply: 2)
528. GST-tagged proteins - (reply: 1)
529. Protino® Ni-IDA Packed Columns - Can you re-use them? (reply: 1)
530. immunoprecipation - (reply: 1)
531. Ni-NTA his tag protein purification - (reply: 1)
532. Weird Induction Problem - (reply: 5)
533. cloning of non-coding region for protein expression - protein expression (reply: 1)
534. from pet-SUMO to pet-28 - Expression problems with pet SUMO (reply: 2)
535. Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
536. Protein expression - (reply: 4)
537. EDTA bacterial cell lysate - (reply: 2)
538. Trouble With HIS affinity column (Ni+) binding - (reply: 1)
539. Problems with affinity purification with M2 beads - (reply: 2)
540. Expression problem using invitrogen pRSET C vector - (reply: 5)

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