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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
511. Expressing 4 proteins in an operon - (reply: 6)
512. More His-tag problems - (reply: 4)
513. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
514. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
515. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
516. Protein precipitates upon dialysis - (reply: 5)
517. membrane protein aggregation - (reply: 1)
518. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
519. His Tag Protein Purification - (reply: 6)
520. Amylose bound protein elution - (reply: 1)
521. hey guys,what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
522. Soluble aggregates - (reply: 3)
523. insect protein expression - (reply: 1)
524. Can you store TNT translation products? - (reply: 1)
525. How to pack the FPLC column - (reply: 2)
526. Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
527. Sonication issues! - (reply: 3)
528. complete gene for expression? - (reply: 2)
529. buffer for membrane protein - (reply: 1)
530. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
531. Question: Bradford assay - buffer precipitates - KCl and HEPES preciptation with Bradford reagent (reply: 3)
532. membrane protein purification - (reply: 6)
533. Protein expression failing - (reply: 1)
534. How does aggregation influence absorbtion at 280nm? - (reply: 1)
535. Buttery Popcorn Odor in Contaminated Expression Prep - (reply: 3)
536. Self-made Protease inhibitor cocktail - PMSF, EDTA, Benzamidine (reply: 4)
537. Contamination in GST fusion protein purification - (reply: 2)
538. GST-tagged proteins - (reply: 1)
539. Protino® Ni-IDA Packed Columns - Can you re-use them? (reply: 1)
540. immunoprecipation - (reply: 1)

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