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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
511. GST-tagged proteins - (reply: 1)
512. Protino® Ni-IDA Packed Columns - Can you re-use them? (reply: 1)
513. immunoprecipation - (reply: 1)
514. Ni-NTA his tag protein purification - (reply: 1)
515. Weird Induction Problem - (reply: 5)
516. cloning of non-coding region for protein expression - protein expression (reply: 1)
517. from pet-SUMO to pet-28 - Expression problems with pet SUMO (reply: 2)
518. Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
519. Protein expression - (reply: 4)
520. EDTA bacterial cell lysate - (reply: 2)
521. Trouble With HIS affinity column (Ni+) binding - (reply: 1)
522. Problems with affinity purification with M2 beads - (reply: 2)
523. Expression problem using invitrogen pRSET C vector - (reply: 5)
524. Vector containing N-his tag and TEV - (reply: 1)
525. Protein expression using invitrogen pSecTag2 - (reply: 2)
526. Problems with nuclear extraction - (reply: 1)
527. His-tagged protein runs much faster than expected - 6His-Tagged CcpA protein runs around 26 kD whereas I expect to run 37 (reply: 7)
528. Removing phosphates - (reply: 2)
529. protein going to inclusion bodies - protein going to inclusion bodies (reply: 1)
530. Looking for endogenus expression by using custom antibody - (reply: 5)
531. Adventitious chemical binding - Unwanted chemicals in protein solution (reply: 2)
532. More than one protein induced - (reply: 4)
533. Protein aggregates - Monomeric fraction varies with wavelength (reply: 1)
534. water withdrawal with PEG20.000 - Does it drag glycerol along too?-PLEASE HELP! (reply: 1)
535. Troubleshooting GST purification - (reply: 4)
536. Amount of inoculum? - for protein expression in E.coli (reply: 3)
537. BL21A or simliar - (reply: 2)
538. Role of imidazole in lysis buffer - for obtaining recombinant his-tag protein (reply: 4)
539. protein expression (temperature issue) - trying to express soluble protein at lower temperature (reply: 17)
540. Can GST-tag be separated from GST-fusion protein - (reply: 1)

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