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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
511. complete gene for expression? - (reply: 2)
512. buffer for membrane protein - (reply: 1)
513. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
514. Question: Bradford assay - buffer precipitates - KCl and HEPES preciptation with Bradford reagent (reply: 3)
515. membrane protein purification - (reply: 6)
516. Protein expression failing - (reply: 1)
517. How does aggregation influence absorbtion at 280nm? - (reply: 1)
518. Buttery Popcorn Odor in Contaminated Expression Prep - (reply: 3)
519. Self-made Protease inhibitor cocktail - PMSF, EDTA, Benzamidine (reply: 4)
520. Contamination in GST fusion protein purification - (reply: 2)
521. GST-tagged proteins - (reply: 1)
522. Protino® Ni-IDA Packed Columns - Can you re-use them? (reply: 1)
523. immunoprecipation - (reply: 1)
524. Ni-NTA his tag protein purification - (reply: 1)
525. Weird Induction Problem - (reply: 5)
526. cloning of non-coding region for protein expression - protein expression (reply: 1)
527. from pet-SUMO to pet-28 - Expression problems with pet SUMO (reply: 2)
528. Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
529. Protein expression - (reply: 4)
530. EDTA bacterial cell lysate - (reply: 2)
531. Trouble With HIS affinity column (Ni+) binding - (reply: 1)
532. Problems with affinity purification with M2 beads - (reply: 2)
533. Expression problem using invitrogen pRSET C vector - (reply: 5)
534. Vector containing N-his tag and TEV - (reply: 1)
535. Protein expression using invitrogen pSecTag2 - (reply: 2)
536. Problems with nuclear extraction - (reply: 1)
537. His-tagged protein runs much faster than expected - 6His-Tagged CcpA protein runs around 26 kD whereas I expect to run 37 (reply: 7)
538. Removing phosphates - (reply: 2)
539. protein going to inclusion bodies - protein going to inclusion bodies (reply: 1)
540. Looking for endogenus expression by using custom antibody - (reply: 5)

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