Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
511. Plyse cell and DH5a - (reply: 1)
512. Quality of aliquot for SDS-page in protein purification - When is pure pure enough? (reply: 1)
513. Electroelution with no electroeluter - (reply: 3)
514. reusing Ni NTA - (reply: 2)
515. Low expression: What else can I do? - Using pET23a in BL21 PlysS (reply: 9)
516. Changing dialysis buffer every half hour and then dialysis ON - protein precipitation prevention (reply: 1)
517. Checking For Protein Expression - (reply: 3)
518. How do I get rid of this contaminant? - His-tagged protein eluted with 300mM imidazole (reply: 13)
519. Is it possible? Harsh sonificiation conditions cause degradation of my protein - (reply: 3)
520. Low Thrombin Cleavage Efficiency Problem - I am trying to cleave a GST tagged protein(about 35 kDa + 26 kDa GST) (reply: 1)
521. Throbin cleavage declines with older protein? - It used to work, now doesn't! (reply: 1)
522. Couple questions about GST purification - (reply: 16)
523. Immunoprecipitation - (reply: 12)
524. Newbie question: how to determine protein concentration after IP? - (reply: 8)
525. protein extraction using trizol - can we replace guanidine cholride with other denaturants? (reply: 1)
526. High pressure on Ni-NTA beads - (reply: 2)
527. affinity column problems - (reply: 1)
528. Desalting protein - (reply: 7)
529. Trypsin Expression and Purification - (reply: 3)
530. seeking help in stable cell line generation - (reply: 3)
531. BL 21 cells - (reply: 4)
532. Thiamine HCl 1M solution? - can I store it at RT? (reply: 2)
533. Low level of expression - in pASK-IBA7 vector (reply: 6)
534. dnase treatment before his-tag purification - (reply: 3)
535. protein aggregation problem - (reply: 3)
536. protein precipitates out during concentrating it - (reply: 2)
537. how to put in evidence protein after IP - (reply: 2)
538. GST-fusion protein purification - How to purify it? (reply: 9)
539. IPTG - (reply: 2)
540. Protein lysis buffer - (reply: 4)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20