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361. Should I worry about endotoxin? - (reply: 4)
362. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
363. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
364. Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
365. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
366. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
367. Western Blot of Large Protein Help - (reply: 1)
368. how to make protein soluble? - protein solubility (reply: 4)
369. protein yield is very low after purification - protein purification (reply: 9)
370. FZR1 PROTEIN EXPRESSION - (reply: 1)
371. Membrane solubilisation - (reply: 2)
372. Heterologous protein extraction in yeast for enzymatic activity test - (reply: 5)
373. His tag protein purification - (reply: 7)
374. Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
375. Protein precipitating need help choosing suitable buffering system - (reply: 3)
376. Expressing human protein in E.Coli - (reply: 12)
377. Sample preparation for SDS from detergent insoluble fraction (Pellet). - (reply: 2)
378. Help with GST protein purification - (reply: 4)
379. Antiobiotics for protein expression - (reply: 3)
380. how to extract proteins from HEK293 - I need your experience (reply: 3)
381. Identification of purity of the protein - (reply: 2)
382. Polysorbate 80 (Tween 80) removal - (reply: 13)
383. Maltose Binding Protein Sequence - A sequence for the Maltose Binding Protein. (reply: 1)
384. nuclear extraction of blood cells - (reply: 2)
385. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
386. volume to load sample for affinity chromatography - (reply: 6)
387. Reporter gene to test new promotor - (reply: 1)
388. Protein Phosphorylation - (reply: 2)
389. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
390. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
362. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
363. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
364. Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
365. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
366. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
367. Western Blot of Large Protein Help - (reply: 1)
368. how to make protein soluble? - protein solubility (reply: 4)
369. protein yield is very low after purification - protein purification (reply: 9)
370. FZR1 PROTEIN EXPRESSION - (reply: 1)
371. Membrane solubilisation - (reply: 2)
372. Heterologous protein extraction in yeast for enzymatic activity test - (reply: 5)
373. His tag protein purification - (reply: 7)
374. Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
375. Protein precipitating need help choosing suitable buffering system - (reply: 3)
376. Expressing human protein in E.Coli - (reply: 12)
377. Sample preparation for SDS from detergent insoluble fraction (Pellet). - (reply: 2)
378. Help with GST protein purification - (reply: 4)
379. Antiobiotics for protein expression - (reply: 3)
380. how to extract proteins from HEK293 - I need your experience (reply: 3)
381. Identification of purity of the protein - (reply: 2)
382. Polysorbate 80 (Tween 80) removal - (reply: 13)
383. Maltose Binding Protein Sequence - A sequence for the Maltose Binding Protein. (reply: 1)
384. nuclear extraction of blood cells - (reply: 2)
385. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
386. volume to load sample for affinity chromatography - (reply: 6)
387. Reporter gene to test new promotor - (reply: 1)
388. Protein Phosphorylation - (reply: 2)
389. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
390. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)