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361. His-tagged protein purification problem - (reply: 2)
362. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
363. Signal Peptides - To leave or not to leave (reply: 2)
364. Low expression of pET21a construct - (reply: 6)
365. GST-fusion protein degrades in-vivo - (reply: 3)
366. Inclusion body purification - (reply: 1)
367. help with protein elution - (reply: 1)
368. where are they on SDS-PAGE gel of Co-IPed complex??? - (reply: 1)
369. Active protein extract from corn - (reply: 2)
370. How to prepare high quality brain tissue lysate? - Will high salt lysis buffer be helpful? (reply: 1)
371. Reduced Glutathione affects thrombin activity? - (reply: 2)
372. You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)
373. Should I worry about endotoxin? - (reply: 4)
374. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
375. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
376. Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
377. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
378. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
379. Western Blot of Large Protein Help - (reply: 1)
380. how to make protein soluble? - protein solubility (reply: 4)
381. protein yield is very low after purification - protein purification (reply: 9)
382. FZR1 PROTEIN EXPRESSION - (reply: 1)
383. Membrane solubilisation - (reply: 2)
384. Heterologous protein extraction in yeast for enzymatic activity test - (reply: 5)
385. His tag protein purification - (reply: 7)
386. Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
387. Protein precipitating need help choosing suitable buffering system - (reply: 3)
388. Expressing human protein in E.Coli - (reply: 12)
389. Sample preparation for SDS from detergent insoluble fraction (Pellet). - (reply: 2)
390. Help with GST protein purification - (reply: 4)
362. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
363. Signal Peptides - To leave or not to leave (reply: 2)
364. Low expression of pET21a construct - (reply: 6)
365. GST-fusion protein degrades in-vivo - (reply: 3)
366. Inclusion body purification - (reply: 1)
367. help with protein elution - (reply: 1)
368. where are they on SDS-PAGE gel of Co-IPed complex??? - (reply: 1)
369. Active protein extract from corn - (reply: 2)
370. How to prepare high quality brain tissue lysate? - Will high salt lysis buffer be helpful? (reply: 1)
371. Reduced Glutathione affects thrombin activity? - (reply: 2)
372. You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)
373. Should I worry about endotoxin? - (reply: 4)
374. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
375. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
376. Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
377. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
378. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
379. Western Blot of Large Protein Help - (reply: 1)
380. how to make protein soluble? - protein solubility (reply: 4)
381. protein yield is very low after purification - protein purification (reply: 9)
382. FZR1 PROTEIN EXPRESSION - (reply: 1)
383. Membrane solubilisation - (reply: 2)
384. Heterologous protein extraction in yeast for enzymatic activity test - (reply: 5)
385. His tag protein purification - (reply: 7)
386. Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
387. Protein precipitating need help choosing suitable buffering system - (reply: 3)
388. Expressing human protein in E.Coli - (reply: 12)
389. Sample preparation for SDS from detergent insoluble fraction (Pellet). - (reply: 2)
390. Help with GST protein purification - (reply: 4)