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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
361. Zeocine resistence, pichia pastoris - (reply: 3)
362. Problem with MBP and GST tag purification - (reply: 1)
363. concentrating protein by method other than tff - (reply: 1)
364. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
365. M2 Flag purification - (reply: 2)
366. Quantitating IP'd proteins - (reply: 1)
367. Removal of Tightly Bound Nucleic Acids - (reply: 3)
368. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
369. Recombinant protein aggregation after purification - (reply: 2)
370. average total protein content per 293t cell - (reply: 2)
371. Problems with Preparative Isoelectrofocusing (minirotofor) - (reply: 1)
372. expression of MBP fusion protein - leaky and very low yield (reply: 2)
373. Calmodulin binding assay - (reply: 1)
374. strange problem with His purification - (reply: 1)
375. E.coli expression - (reply: 5)
376. why my protein precipitates? - (reply: 2)
377. DTT in GST elution buffer - (reply: 1)
378. vectors and his-tags - (reply: 3)
379. SDS boiled samples - (reply: 1)
380. E coli lysis by lysozyme - (reply: 2)
381. His-tag protein expression - (reply: 1)
382. SDS-PAGE loading dye - (reply: 1)
383. How to concentrate the protein - (reply: 4)
384. protein overexpression - factors affecting overexpression (reply: 3)
385. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
386. e coli strain selection for protein expression - (reply: 2)
387. Problem clariflying/filtering insect cell lysate - (reply: 2)
388. Ni column changing colors - (reply: 2)
389. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
390. Problem with Histag purification - (reply: 1)

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