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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
361. Sample preparation for SDS from detergent insoluble fraction (Pellet). - (reply: 2)
362. Help with GST protein purification - (reply: 4)
363. Antiobiotics for protein expression - (reply: 3)
364. how to extract proteins from HEK293 - I need your experience (reply: 3)
365. Identification of purity of the protein - (reply: 2)
366. Polysorbate 80 (Tween 80) removal - (reply: 13)
367. Maltose Binding Protein Sequence - A sequence for the Maltose Binding Protein. (reply: 1)
368. nuclear extraction of blood cells - (reply: 2)
369. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
370. volume to load sample for affinity chromatography - (reply: 6)
371. Reporter gene to test new promotor - (reply: 1)
372. Protein Phosphorylation - (reply: 2)
373. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
374. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
375. Folding - (reply: 2)
376. C-terminal Cleavage During Overexpression - (reply: 3)
377. FOR immunoprecipitation in plant , flag tag or His tag - (reply: 2)
378. Loss of protein upon concentration - (reply: 4)
379. Problems with Bacterial Induction: NO Induction - (reply: 1)
380. His-tag protein purification - aggregation of protein (reply: 5)
381. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
382. Coimmunoprecipitation using Ni-NTA, My non-his-tagged target bind to beads - (reply: 3)
383. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
384. Decrease in concentration after protein purification - (reply: 3)
385. Ammonium sulphate precipitation Problem - Precipitants couldnt be seen on SDS-PAGE and WB (reply: 9)
386. Bacterial Induction: Same concentration of protein samples? - (reply: 1)
387. ways to stop soluble aggregation - (reply: 9)
388. need protocol on purfication of allinase from raw garlic - (reply: 2)
389. labeling a protein - (reply: 3)
390. strep-tag II purification not pure - how to make it pure with one step purification or rather do two step (reply: 3)

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