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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
361. Expressing human protein in E.Coli - (reply: 12)
362. Sample preparation for SDS from detergent insoluble fraction (Pellet). - (reply: 2)
363. Help with GST protein purification - (reply: 4)
364. Antiobiotics for protein expression - (reply: 3)
365. how to extract proteins from HEK293 - I need your experience (reply: 3)
366. Identification of purity of the protein - (reply: 2)
367. Polysorbate 80 (Tween 80) removal - (reply: 13)
368. Maltose Binding Protein Sequence - A sequence for the Maltose Binding Protein. (reply: 1)
369. nuclear extraction of blood cells - (reply: 2)
370. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
371. volume to load sample for affinity chromatography - (reply: 6)
372. Reporter gene to test new promotor - (reply: 1)
373. Protein Phosphorylation - (reply: 2)
374. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
375. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
376. Folding - (reply: 2)
377. C-terminal Cleavage During Overexpression - (reply: 3)
378. FOR immunoprecipitation in plant , flag tag or His tag - (reply: 2)
379. Loss of protein upon concentration - (reply: 4)
380. Problems with Bacterial Induction: NO Induction - (reply: 1)
381. His-tag protein purification - aggregation of protein (reply: 5)
382. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
383. Coimmunoprecipitation using Ni-NTA, My non-his-tagged target bind to beads - (reply: 3)
384. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
385. Decrease in concentration after protein purification - (reply: 3)
386. Ammonium sulphate precipitation Problem - Precipitants couldnt be seen on SDS-PAGE and WB (reply: 9)
387. Bacterial Induction: Same concentration of protein samples? - (reply: 1)
388. ways to stop soluble aggregation - (reply: 9)
389. need protocol on purfication of allinase from raw garlic - (reply: 2)
390. labeling a protein - (reply: 3)

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