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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
361. Low expression of pET21a construct - (reply: 6)
362. GST-fusion protein degrades in-vivo - (reply: 3)
363. Inclusion body purification - (reply: 1)
364. help with protein elution - (reply: 1)
365. where are they on SDS-PAGE gel of Co-IPed complex??? - (reply: 1)
366. Active protein extract from corn - (reply: 2)
367. How to prepare high quality brain tissue lysate? - Will high salt lysis buffer be helpful? (reply: 1)
368. Reduced Glutathione affects thrombin activity? - (reply: 2)
369. You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)
370. Should I worry about endotoxin? - (reply: 4)
371. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
372. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
373. Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
374. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
375. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
376. Western Blot of Large Protein Help - (reply: 1)
377. how to make protein soluble? - protein solubility (reply: 4)
378. protein yield is very low after purification - protein purification (reply: 9)
379. FZR1 PROTEIN EXPRESSION - (reply: 1)
380. Membrane solubilisation - (reply: 2)
381. Heterologous protein extraction in yeast for enzymatic activity test - (reply: 5)
382. His tag protein purification - (reply: 7)
383. Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
384. Protein precipitating need help choosing suitable buffering system - (reply: 3)
385. Expressing human protein in E.Coli - (reply: 12)
386. Sample preparation for SDS from detergent insoluble fraction (Pellet). - (reply: 2)
387. Help with GST protein purification - (reply: 4)
388. Antiobiotics for protein expression - (reply: 3)
389. how to extract proteins from HEK293 - I need your experience (reply: 3)
390. Identification of purity of the protein - (reply: 2)

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