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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
361. IPTG induction not observable or noticeable - (reply: 2)
362. Problem with protein purification By FPLC - (reply: 4)
363. Protein precipitation with TCA? - Protocols, suggestions, ideas, experiences (reply: 2)
364. His-tag proteins not eluted from Ni-Beads - (reply: 2)
365. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
366. Recombinant Antibody Production -Reducing / Non-reducing SDS page conditions - (reply: 2)
367. trouble with protein expression of high AT rich gene - (reply: 1)
368. Preparation of Purified Inclusion Bodies - (reply: 2)
369. Denaturing IB in 8 M Urea - (reply: 3)
370. Promega IVT Problems - Low expression (reply: 1)
371. weak bands - western blots (reply: 1)
372. problem in M9 media - m9 media (reply: 4)
373. Help for a purificaltion newbie - (reply: 2)
374. Immunioprecipitation from Jurkat cells?? - (reply: 1)
375. Problems with Soluble and Insoluble Protein Fraction - (reply: 4)
376. antibody HRP - (reply: 2)
377. Western blot transfer weak - (reply: 1)
378. Home-made Size-exclusion Columns? - (reply: 1)
379. how to get pure protein - (reply: 1)
380. co-Immunoprecipitation control question - co-IP (reply: 1)
381. to get protein in cytosol from inclusion bodies - (reply: 4)
382. Inclusion bodies in e.coli - (reply: 1)
383. lysis of e.coli by sonication - (reply: 2)
384. Looking for precipitation method to reduce the volume of my protein complex elu - TCA precipitation seems to be hard to be resolved again (reply: 6)
385. Protein expression - (reply: 8)
386. problem with ion exchange puification - protein not binding (reply: 3)
387. Protein Purification by precipitation - (reply: 2)
388. refolding proteins using dialysis - (reply: 1)
389. protein refolding - (reply: 1)
390. Zeocine resistence, pichia pastoris - (reply: 3)

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