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361. How to prepare high quality brain tissue lysate? - Will high salt lysis buffer be helpful? (reply: 1)
362. Reduced Glutathione affects thrombin activity? - (reply: 2)
363. You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)
364. Should I worry about endotoxin? - (reply: 4)
365. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
366. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
367. Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
368. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
369. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
370. Western Blot of Large Protein Help - (reply: 1)
371. how to make protein soluble? - protein solubility (reply: 4)
372. protein yield is very low after purification - protein purification (reply: 9)
373. FZR1 PROTEIN EXPRESSION - (reply: 1)
374. Membrane solubilisation - (reply: 2)
375. Heterologous protein extraction in yeast for enzymatic activity test - (reply: 5)
376. His tag protein purification - (reply: 7)
377. Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
378. Protein precipitating need help choosing suitable buffering system - (reply: 3)
379. Expressing human protein in E.Coli - (reply: 12)
380. Sample preparation for SDS from detergent insoluble fraction (Pellet). - (reply: 2)
381. Help with GST protein purification - (reply: 4)
382. Antiobiotics for protein expression - (reply: 3)
383. how to extract proteins from HEK293 - I need your experience (reply: 3)
384. Identification of purity of the protein - (reply: 2)
385. Polysorbate 80 (Tween 80) removal - (reply: 13)
386. Maltose Binding Protein Sequence - A sequence for the Maltose Binding Protein. (reply: 1)
387. nuclear extraction of blood cells - (reply: 2)
388. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
389. volume to load sample for affinity chromatography - (reply: 6)
390. Reporter gene to test new promotor - (reply: 1)
362. Reduced Glutathione affects thrombin activity? - (reply: 2)
363. You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)
364. Should I worry about endotoxin? - (reply: 4)
365. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
366. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
367. Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
368. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
369. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
370. Western Blot of Large Protein Help - (reply: 1)
371. how to make protein soluble? - protein solubility (reply: 4)
372. protein yield is very low after purification - protein purification (reply: 9)
373. FZR1 PROTEIN EXPRESSION - (reply: 1)
374. Membrane solubilisation - (reply: 2)
375. Heterologous protein extraction in yeast for enzymatic activity test - (reply: 5)
376. His tag protein purification - (reply: 7)
377. Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
378. Protein precipitating need help choosing suitable buffering system - (reply: 3)
379. Expressing human protein in E.Coli - (reply: 12)
380. Sample preparation for SDS from detergent insoluble fraction (Pellet). - (reply: 2)
381. Help with GST protein purification - (reply: 4)
382. Antiobiotics for protein expression - (reply: 3)
383. how to extract proteins from HEK293 - I need your experience (reply: 3)
384. Identification of purity of the protein - (reply: 2)
385. Polysorbate 80 (Tween 80) removal - (reply: 13)
386. Maltose Binding Protein Sequence - A sequence for the Maltose Binding Protein. (reply: 1)
387. nuclear extraction of blood cells - (reply: 2)
388. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
389. volume to load sample for affinity chromatography - (reply: 6)
390. Reporter gene to test new promotor - (reply: 1)