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361. IMPACT system purification problem - (reply: 1)
362. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
363. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
364. How to normalize my samples before I transfect my cells - (reply: 6)
365. FBS interference in SDS PAGE - (reply: 3)
366. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
367. What make voltage not going up for the 3rd step in running IEF? - (reply: 2)
368. Help...purify and concentrate protein for IEF - (reply: 1)
369. preference of N/C terminus for tagging - (reply: 2)
370. NI-NTA purification - (reply: 2)
371. Could a 30aa peptides(3×flag+6×his） be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)
372. Ampicillin Mess-up(Experiment in progress atm) - Experiment still valid? (reply: 1)
373. RNA immunoprecipitation...HELP! - (reply: 4)
374. Dialysis - A question about buffers (reply: 6)
375. Purification problems his-tag - I have tried everything (reply: 3)
376. GST-tagged protein binds to column but is not eluted - (reply: 1)
377. urea-denatured protein precipitates after dialysis - (reply: 1)
378. Expressing 4 proteins in an operon - (reply: 6)
379. More His-tag problems - (reply: 4)
380. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
381. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
382. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
383. Protein precipitates upon dialysis - (reply: 5)
384. membrane protein aggregation - (reply: 1)
385. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
386. His Tag Protein Purification - (reply: 6)
387. Amylose bound protein elution - (reply: 1)
388. hey guys，what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
389. Soluble aggregates - (reply: 3)
390. insect protein expression - (reply: 1)
362. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
363. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
364. How to normalize my samples before I transfect my cells - (reply: 6)
365. FBS interference in SDS PAGE - (reply: 3)
366. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
367. What make voltage not going up for the 3rd step in running IEF? - (reply: 2)
368. Help...purify and concentrate protein for IEF - (reply: 1)
369. preference of N/C terminus for tagging - (reply: 2)
370. NI-NTA purification - (reply: 2)
371. Could a 30aa peptides(3×flag+6×his） be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)
372. Ampicillin Mess-up(Experiment in progress atm) - Experiment still valid? (reply: 1)
373. RNA immunoprecipitation...HELP! - (reply: 4)
374. Dialysis - A question about buffers (reply: 6)
375. Purification problems his-tag - I have tried everything (reply: 3)
376. GST-tagged protein binds to column but is not eluted - (reply: 1)
377. urea-denatured protein precipitates after dialysis - (reply: 1)
378. Expressing 4 proteins in an operon - (reply: 6)
379. More His-tag problems - (reply: 4)
380. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
381. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
382. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
383. Protein precipitates upon dialysis - (reply: 5)
384. membrane protein aggregation - (reply: 1)
385. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
386. His Tag Protein Purification - (reply: 6)
387. Amylose bound protein elution - (reply: 1)
388. hey guys，what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
389. Soluble aggregates - (reply: 3)
390. insect protein expression - (reply: 1)