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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
361. Heterologous protein extraction in yeast for enzymatic activity test - (reply: 5)
362. His tag protein purification - (reply: 7)
363. Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
364. Protein precipitating need help choosing suitable buffering system - (reply: 3)
365. Expressing human protein in E.Coli - (reply: 12)
366. Sample preparation for SDS from detergent insoluble fraction (Pellet). - (reply: 2)
367. Help with GST protein purification - (reply: 4)
368. Antiobiotics for protein expression - (reply: 3)
369. how to extract proteins from HEK293 - I need your experience (reply: 3)
370. Identification of purity of the protein - (reply: 2)
371. Polysorbate 80 (Tween 80) removal - (reply: 13)
372. Maltose Binding Protein Sequence - A sequence for the Maltose Binding Protein. (reply: 1)
373. nuclear extraction of blood cells - (reply: 2)
374. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
375. volume to load sample for affinity chromatography - (reply: 6)
376. Reporter gene to test new promotor - (reply: 1)
377. Protein Phosphorylation - (reply: 2)
378. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
379. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
380. Folding - (reply: 2)
381. C-terminal Cleavage During Overexpression - (reply: 3)
382. FOR immunoprecipitation in plant , flag tag or His tag - (reply: 2)
383. Loss of protein upon concentration - (reply: 4)
384. Problems with Bacterial Induction: NO Induction - (reply: 1)
385. His-tag protein purification - aggregation of protein (reply: 5)
386. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
387. Coimmunoprecipitation using Ni-NTA, My non-his-tagged target bind to beads - (reply: 3)
388. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
389. Decrease in concentration after protein purification - (reply: 3)
390. Ammonium sulphate precipitation Problem - Precipitants couldnt be seen on SDS-PAGE and WB (reply: 9)

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