Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
361. protein refolding - (reply: 1)
362. Zeocine resistence, pichia pastoris - (reply: 3)
363. Problem with MBP and GST tag purification - (reply: 1)
364. concentrating protein by method other than tff - (reply: 1)
365. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
366. M2 Flag purification - (reply: 2)
367. Quantitating IP'd proteins - (reply: 1)
368. Removal of Tightly Bound Nucleic Acids - (reply: 3)
369. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
370. Recombinant protein aggregation after purification - (reply: 2)
371. average total protein content per 293t cell - (reply: 2)
372. Problems with Preparative Isoelectrofocusing (minirotofor) - (reply: 1)
373. expression of MBP fusion protein - leaky and very low yield (reply: 2)
374. Calmodulin binding assay - (reply: 1)
375. strange problem with His purification - (reply: 1)
376. E.coli expression - (reply: 5)
377. why my protein precipitates? - (reply: 2)
378. DTT in GST elution buffer - (reply: 1)
379. vectors and his-tags - (reply: 3)
380. SDS boiled samples - (reply: 1)
381. E coli lysis by lysozyme - (reply: 2)
382. His-tag protein expression - (reply: 1)
383. SDS-PAGE loading dye - (reply: 1)
384. How to concentrate the protein - (reply: 4)
385. protein overexpression - factors affecting overexpression (reply: 3)
386. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
387. e coli strain selection for protein expression - (reply: 2)
388. Problem clariflying/filtering insect cell lysate - (reply: 2)
389. Ni column changing colors - (reply: 2)
390. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19