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New Forum Archives (2009-): :
571. membrane protein extraction buffer - (reply: 1)
573. Ni-NTA beads and dialysis tubing - Purification using nickel beads (reply: 4)
575. protein is degraded after purification - (reply: 2)
577. Problem with Protein elution using GST sepharose 4B - (reply: 1)
579. Plasmid instability in E.coli BL21? - (reply: 3)
581. Plyse cell and DH5a - (reply: 1)
583. Electroelution with no electroeluter - (reply: 3)
585. Low expression: What else can I do? - Using pET23a in BL21 PlysS (reply: 9)
587. Checking For Protein Expression - (reply: 3)
589. Is it possible? Harsh sonificiation conditions cause degradation of my protein - (reply: 3)
591. Throbin cleavage declines with older protein? - It used to work, now doesn't! (reply: 1)
593. Immunoprecipitation - (reply: 12)
595. protein extraction using trizol - can we replace guanidine cholride with other denaturants? (reply: 1)
597. affinity column problems - (reply: 1)
599. Trypsin Expression and Purification - (reply: 3)
573. Ni-NTA beads and dialysis tubing - Purification using nickel beads (reply: 4)
575. protein is degraded after purification - (reply: 2)
577. Problem with Protein elution using GST sepharose 4B - (reply: 1)
579. Plasmid instability in E.coli BL21? - (reply: 3)
581. Plyse cell and DH5a - (reply: 1)
583. Electroelution with no electroeluter - (reply: 3)
585. Low expression: What else can I do? - Using pET23a in BL21 PlysS (reply: 9)
587. Checking For Protein Expression - (reply: 3)
589. Is it possible? Harsh sonificiation conditions cause degradation of my protein - (reply: 3)
591. Throbin cleavage declines with older protein? - It used to work, now doesn't! (reply: 1)
593. Immunoprecipitation - (reply: 12)
595. protein extraction using trizol - can we replace guanidine cholride with other denaturants? (reply: 1)
597. affinity column problems - (reply: 1)
599. Trypsin Expression and Purification - (reply: 3)