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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
211. Problems with overnights - (reply: 5)
212. problem in extracting the protein - (reply: 1)
213. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
214. Protein Purification from Polyacrylamide Gels - (reply: 3)
215. Protein Expression vector with small tag - (reply: 1)
216. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
217. Albumin Removal From BSA - (reply: 1)
218. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
219. Insulin production - (reply: 2)
220. High Yield Protein Expression 10g/L - (reply: 5)
221. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
222. GST- tagged protein expressed in insoluble - (reply: 5)
223. Scaling up reagents for large scale immunoprecipitation - (reply: 1)
224. Prepare protein standards for concentration determination - (reply: 1)
225. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)
226. Tagged recombinant protein has different mobility? - (reply: 7)
227. My protein doesn't bind to the Ni-NTA column - (reply: 11)
228. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
229. TCA IgG precipitation - (reply: 2)
230. Does triple labeled protein precipitate more easily? - (reply: 2)
231. High gravity crush beads ? - (reply: 1)
232. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
233. how do GST-tags affect gel filtration results? - (reply: 5)
234. protein extraction from human tissue (very small) to do ELISA - (reply: 5)
235. Stable protein expression in Vero cells - (reply: 2)
236. Which kind of ATP do you usually use in you experiment? - (reply: 2)
237. protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
238. GST-tagged protein expression problem! :-( - (reply: 4)
239. Better (quicker) way to pipet glycerol? - (reply: 3)
240. How OD600 influence on IPTG induction and protein expression - (reply: 5)

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