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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
211. protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
212. GST-tagged protein expression problem! :-( - (reply: 4)
213. Better (quicker) way to pipet glycerol? - (reply: 3)
214. How OD600 influence on IPTG induction and protein expression - (reply: 5)
215. does EGFP dimerize? - (reply: 3)
216. IPTG induction doesn't work:( - (reply: 3)
217. question about transformation - (reply: 1)
218. Mouse IgG1 purification - Protein G - (reply: 1)
219. Make my own BL21-CodonPlus(DE3)? - (reply: 3)
220. what will happen if you let your affinity column beads dry? - (reply: 1)
221. pichia epression problem - (reply: 1)
222. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
223. Multiple protein bands after expression - (reply: 6)
224. his-tag prufication lysis problem:( - (reply: 4)
225. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
226. co-eluted protein with his-tag protein - (reply: 1)
227. Help with His-Tag protein purification - (reply: 2)
228. Discrepancy between tissue and cell culture - (reply: 1)
229. Expression and Lysozyme - (reply: 3)
230. Rosetta-gami B Incubation Time - (reply: 1)
231. Decreased protein yields after successive passaging? - (reply: 1)
232. How to distinguis soluble from insoluble recombinant proteins produced in E. col - (reply: 1)
233. FPLC - why is it so hard to filter my crude extract? - (reply: 1)
234. Lysis Buffer turns yellow after phos inhib added. - (reply: 1)
235. can Protamine sulfate inhibit or degrage a protein? - (reply: 1)
236. how much time for prepared gel to keep bfore loading? - (reply: 5)
237. plant extraction procedure for protein - (reply: 3)
238. brain tissue homogenization with sample buffer?? - (reply: 2)
239. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
240. Reversed-Phase chromatography for protein purification - (reply: 3)

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