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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
211. High gravity crush beads ? - (reply: 1)
212. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
213. how do GST-tags affect gel filtration results? - (reply: 5)
214. protein extraction from human tissue (very small) to do ELISA - (reply: 5)
215. Stable protein expression in Vero cells - (reply: 2)
216. Which kind of ATP do you usually use in you experiment? - (reply: 2)
217. protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
218. GST-tagged protein expression problem! :-( - (reply: 4)
219. Better (quicker) way to pipet glycerol? - (reply: 3)
220. How OD600 influence on IPTG induction and protein expression - (reply: 5)
221. does EGFP dimerize? - (reply: 3)
222. IPTG induction doesn't work:( - (reply: 3)
223. question about transformation - (reply: 1)
224. Mouse IgG1 purification - Protein G - (reply: 1)
225. Make my own BL21-CodonPlus(DE3)? - (reply: 3)
226. what will happen if you let your affinity column beads dry? - (reply: 1)
227. pichia epression problem - (reply: 1)
228. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
229. Multiple protein bands after expression - (reply: 6)
230. his-tag prufication lysis problem:( - (reply: 4)
231. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
232. co-eluted protein with his-tag protein - (reply: 1)
233. Help with His-Tag protein purification - (reply: 2)
234. Discrepancy between tissue and cell culture - (reply: 1)
235. Expression and Lysozyme - (reply: 3)
236. Rosetta-gami B Incubation Time - (reply: 1)
237. Decreased protein yields after successive passaging? - (reply: 1)
238. How to distinguis soluble from insoluble recombinant proteins produced in E. col - (reply: 1)
239. FPLC - why is it so hard to filter my crude extract? - (reply: 1)
240. Lysis Buffer turns yellow after phos inhib added. - (reply: 1)

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