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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
211. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
212. TCA IgG precipitation - (reply: 2)
213. Does triple labeled protein precipitate more easily? - (reply: 2)
214. High gravity crush beads ? - (reply: 1)
215. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
216. how do GST-tags affect gel filtration results? - (reply: 5)
217. protein extraction from human tissue (very small) to do ELISA - (reply: 5)
218. Stable protein expression in Vero cells - (reply: 2)
219. Which kind of ATP do you usually use in you experiment? - (reply: 2)
220. protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
221. GST-tagged protein expression problem! :-( - (reply: 4)
222. Better (quicker) way to pipet glycerol? - (reply: 3)
223. How OD600 influence on IPTG induction and protein expression - (reply: 5)
224. does EGFP dimerize? - (reply: 3)
225. IPTG induction doesn't work:( - (reply: 3)
226. question about transformation - (reply: 1)
227. Mouse IgG1 purification - Protein G - (reply: 1)
228. Make my own BL21-CodonPlus(DE3)? - (reply: 3)
229. what will happen if you let your affinity column beads dry? - (reply: 1)
230. pichia epression problem - (reply: 1)
231. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
232. Multiple protein bands after expression - (reply: 6)
233. his-tag prufication lysis problem:( - (reply: 4)
234. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
235. co-eluted protein with his-tag protein - (reply: 1)
236. Help with His-Tag protein purification - (reply: 2)
237. Discrepancy between tissue and cell culture - (reply: 1)
238. Expression and Lysozyme - (reply: 3)
239. Rosetta-gami B Incubation Time - (reply: 1)
240. Decreased protein yields after successive passaging? - (reply: 1)

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