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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
211. how do GST-tags affect gel filtration results? - (reply: 5)
212. protein extraction from human tissue (very small) to do ELISA - (reply: 5)
213. Stable protein expression in Vero cells - (reply: 2)
214. Which kind of ATP do you usually use in you experiment? - (reply: 2)
215. protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
216. GST-tagged protein expression problem! :-( - (reply: 4)
217. Better (quicker) way to pipet glycerol? - (reply: 3)
218. How OD600 influence on IPTG induction and protein expression - (reply: 5)
219. does EGFP dimerize? - (reply: 3)
220. IPTG induction doesn't work:( - (reply: 3)
221. question about transformation - (reply: 1)
222. Mouse IgG1 purification - Protein G - (reply: 1)
223. Make my own BL21-CodonPlus(DE3)? - (reply: 3)
224. what will happen if you let your affinity column beads dry? - (reply: 1)
225. pichia epression problem - (reply: 1)
226. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
227. Multiple protein bands after expression - (reply: 6)
228. his-tag prufication lysis problem:( - (reply: 4)
229. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
230. co-eluted protein with his-tag protein - (reply: 1)
231. Help with His-Tag protein purification - (reply: 2)
232. Discrepancy between tissue and cell culture - (reply: 1)
233. Expression and Lysozyme - (reply: 3)
234. Rosetta-gami B Incubation Time - (reply: 1)
235. Decreased protein yields after successive passaging? - (reply: 1)
236. How to distinguis soluble from insoluble recombinant proteins produced in E. col - (reply: 1)
237. FPLC - why is it so hard to filter my crude extract? - (reply: 1)
238. Lysis Buffer turns yellow after phos inhib added. - (reply: 1)
239. can Protamine sulfate inhibit or degrage a protein? - (reply: 1)
240. how much time for prepared gel to keep bfore loading? - (reply: 5)

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