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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
211. Insulin production - (reply: 2)
212. High Yield Protein Expression 10g/L - (reply: 5)
213. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
214. GST- tagged protein expressed in insoluble - (reply: 5)
215. Scaling up reagents for large scale immunoprecipitation - (reply: 1)
216. Prepare protein standards for concentration determination - (reply: 1)
217. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)
218. Tagged recombinant protein has different mobility? - (reply: 7)
219. My protein doesn't bind to the Ni-NTA column - (reply: 11)
220. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
221. TCA IgG precipitation - (reply: 2)
222. Does triple labeled protein precipitate more easily? - (reply: 2)
223. High gravity crush beads ? - (reply: 1)
224. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
225. how do GST-tags affect gel filtration results? - (reply: 5)
226. protein extraction from human tissue (very small) to do ELISA - (reply: 5)
227. Stable protein expression in Vero cells - (reply: 2)
228. Which kind of ATP do you usually use in you experiment? - (reply: 2)
229. protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
230. GST-tagged protein expression problem! :-( - (reply: 4)
231. Better (quicker) way to pipet glycerol? - (reply: 3)
232. How OD600 influence on IPTG induction and protein expression - (reply: 5)
233. does EGFP dimerize? - (reply: 3)
234. IPTG induction doesn't work:( - (reply: 3)
235. question about transformation - (reply: 1)
236. Mouse IgG1 purification - Protein G - (reply: 1)
237. Make my own BL21-CodonPlus(DE3)? - (reply: 3)
238. what will happen if you let your affinity column beads dry? - (reply: 1)
239. pichia epression problem - (reply: 1)
240. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)

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