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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
211. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)
212. Tagged recombinant protein has different mobility? - (reply: 6)
213. My protein doesn't bind to the Ni-NTA column - (reply: 11)
214. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
215. TCA IgG precipitation - (reply: 2)
216. Does triple labeled protein precipitate more easily? - (reply: 2)
217. High gravity crush beads ? - (reply: 1)
218. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
219. how do GST-tags affect gel filtration results? - (reply: 5)
220. protein extraction from human tissue (very small) to do ELISA - (reply: 5)
221. Stable protein expression in Vero cells - (reply: 2)
222. Which kind of ATP do you usually use in you experiment? - (reply: 2)
223. protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
224. GST-tagged protein expression problem! :-( - (reply: 4)
225. Better (quicker) way to pipet glycerol? - (reply: 3)
226. How OD600 influence on IPTG induction and protein expression - (reply: 5)
227. does EGFP dimerize? - (reply: 3)
228. IPTG induction doesn't work:( - (reply: 3)
229. question about transformation - (reply: 1)
230. Mouse IgG1 purification - Protein G - (reply: 1)
231. Make my own BL21-CodonPlus(DE3)? - (reply: 3)
232. what will happen if you let your affinity column beads dry? - (reply: 1)
233. pichia epression problem - (reply: 1)
234. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
235. Multiple protein bands after expression - (reply: 6)
236. his-tag prufication lysis problem:( - (reply: 4)
237. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
238. co-eluted protein with his-tag protein - (reply: 1)
239. Help with His-Tag protein purification - (reply: 2)
240. Discrepancy between tissue and cell culture - (reply: 1)

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