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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
451. How to pack the FPLC column - (reply: 2)
452. Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
453. Sonication issues! - (reply: 3)
454. complete gene for expression? - (reply: 2)
455. buffer for membrane protein - (reply: 1)
456. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
457. Question: Bradford assay - buffer precipitates - KCl and HEPES preciptation with Bradford reagent (reply: 3)
458. membrane protein purification - (reply: 6)
459. Protein expression failing - (reply: 1)
460. How does aggregation influence absorbtion at 280nm? - (reply: 1)
461. Buttery Popcorn Odor in Contaminated Expression Prep - (reply: 3)
462. Self-made Protease inhibitor cocktail - PMSF, EDTA, Benzamidine (reply: 4)
463. Contamination in GST fusion protein purification - (reply: 2)
464. GST-tagged proteins - (reply: 1)
465. Protino® Ni-IDA Packed Columns - Can you re-use them? (reply: 1)
466. immunoprecipation - (reply: 1)
467. Ni-NTA his tag protein purification - (reply: 1)
468. Weird Induction Problem - (reply: 5)
469. cloning of non-coding region for protein expression - protein expression (reply: 1)
470. from pet-SUMO to pet-28 - Expression problems with pet SUMO (reply: 2)
471. Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
472. Protein expression - (reply: 4)
473. EDTA bacterial cell lysate - (reply: 2)
474. Trouble With HIS affinity column (Ni+) binding - (reply: 1)
475. Problems with affinity purification with M2 beads - (reply: 2)
476. Expression problem using invitrogen pRSET C vector - (reply: 5)
477. Vector containing N-his tag and TEV - (reply: 1)
478. Protein expression using invitrogen pSecTag2 - (reply: 2)
479. Problems with nuclear extraction - (reply: 1)
480. His-tagged protein runs much faster than expected - 6His-Tagged CcpA protein runs around 26 kD whereas I expect to run 37 (reply: 7)

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