Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
451. strange problem with His purification - (reply: 1)
452. E.coli expression - (reply: 5)
453. why my protein precipitates? - (reply: 2)
454. DTT in GST elution buffer - (reply: 1)
455. vectors and his-tags - (reply: 3)
456. SDS boiled samples - (reply: 1)
457. E coli lysis by lysozyme - (reply: 2)
458. His-tag protein expression - (reply: 1)
459. SDS-PAGE loading dye - (reply: 1)
460. How to concentrate the protein - (reply: 4)
461. protein overexpression - factors affecting overexpression (reply: 3)
462. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
463. e coli strain selection for protein expression - (reply: 2)
464. Problem clariflying/filtering insect cell lysate - (reply: 2)
465. Ni column changing colors - (reply: 2)
466. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
467. Problem with Histag purification - (reply: 1)
468. Freezing eluted His tag protein - (reply: 5)
469. Why Ni-NTA beads became clumped after incubation? - Intensive Clumped (reply: 2)
470. His-tag protein lost it's tag - (reply: 2)
471. AnionExchange problem: proteins do not bind - (reply: 12)
472. How much protein should be loaded to the column during the purificiation? - Is less more? ;) (reply: 1)
473. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
474. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
475. Centrifugal Filter Unit - Trying to get a higher concentration. (reply: 3)
476. IMPACT system purification problem - (reply: 1)
477. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
478. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
479. How to normalize my samples before I transfect my cells - (reply: 6)
480. FBS interference in SDS PAGE - (reply: 3)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22