Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
451. E coli lysis by lysozyme - (reply: 2)
452. His-tag protein expression - (reply: 1)
453. SDS-PAGE loading dye - (reply: 1)
454. How to concentrate the protein - (reply: 4)
455. protein overexpression - factors affecting overexpression (reply: 3)
456. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
457. e coli strain selection for protein expression - (reply: 2)
458. Problem clariflying/filtering insect cell lysate - (reply: 2)
459. Ni column changing colors - (reply: 2)
460. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
461. Problem with Histag purification - (reply: 1)
462. Freezing eluted His tag protein - (reply: 5)
463. Why Ni-NTA beads became clumped after incubation? - Intensive Clumped (reply: 2)
464. His-tag protein lost it's tag - (reply: 2)
465. AnionExchange problem: proteins do not bind - (reply: 12)
466. How much protein should be loaded to the column during the purificiation? - Is less more? ;) (reply: 1)
467. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
468. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
469. Centrifugal Filter Unit - Trying to get a higher concentration. (reply: 3)
470. IMPACT system purification problem - (reply: 1)
471. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
472. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
473. How to normalize my samples before I transfect my cells - (reply: 6)
474. FBS interference in SDS PAGE - (reply: 3)
475. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
476. What make voltage not going up for the 3rd step in running IEF? - (reply: 2)
477. Help...purify and concentrate protein for IEF - (reply: 1)
478. preference of N/C terminus for tagging - (reply: 2)
479. NI-NTA purification - (reply: 2)
480. Could a 30aa peptides(3×flag+6×his) be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22