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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
451. Zeocine resistence, pichia pastoris - (reply: 3)
452. Problem with MBP and GST tag purification - (reply: 1)
453. concentrating protein by method other than tff - (reply: 1)
454. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
455. M2 Flag purification - (reply: 2)
456. Quantitating IP'd proteins - (reply: 1)
457. Removal of Tightly Bound Nucleic Acids - (reply: 3)
458. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
459. Recombinant protein aggregation after purification - (reply: 2)
460. average total protein content per 293t cell - (reply: 2)
461. Problems with Preparative Isoelectrofocusing (minirotofor) - (reply: 1)
462. expression of MBP fusion protein - leaky and very low yield (reply: 2)
463. Calmodulin binding assay - (reply: 1)
464. strange problem with His purification - (reply: 1)
465. E.coli expression - (reply: 5)
466. why my protein precipitates? - (reply: 2)
467. DTT in GST elution buffer - (reply: 1)
468. vectors and his-tags - (reply: 3)
469. SDS boiled samples - (reply: 1)
470. E coli lysis by lysozyme - (reply: 2)
471. His-tag protein expression - (reply: 1)
472. SDS-PAGE loading dye - (reply: 1)
473. How to concentrate the protein - (reply: 4)
474. protein overexpression - factors affecting overexpression (reply: 3)
475. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
476. e coli strain selection for protein expression - (reply: 2)
477. Problem clariflying/filtering insect cell lysate - (reply: 2)
478. Ni column changing colors - (reply: 2)
479. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
480. Problem with Histag purification - (reply: 1)

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