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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
451. GST-tagged protein binds to column but is not eluted - (reply: 1)
452. urea-denatured protein precipitates after dialysis - (reply: 1)
453. Expressing 4 proteins in an operon - (reply: 6)
454. More His-tag problems - (reply: 4)
455. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
456. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
457. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
458. Protein precipitates upon dialysis - (reply: 5)
459. membrane protein aggregation - (reply: 1)
460. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
461. His Tag Protein Purification - (reply: 6)
462. Amylose bound protein elution - (reply: 1)
463. hey guys,what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
464. Soluble aggregates - (reply: 3)
465. insect protein expression - (reply: 1)
466. Can you store TNT translation products? - (reply: 1)
467. How to pack the FPLC column - (reply: 2)
468. Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
469. Sonication issues! - (reply: 3)
470. complete gene for expression? - (reply: 2)
471. buffer for membrane protein - (reply: 1)
472. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
473. Question: Bradford assay - buffer precipitates - KCl and HEPES preciptation with Bradford reagent (reply: 3)
474. membrane protein purification - (reply: 6)
475. Protein expression failing - (reply: 1)
476. How does aggregation influence absorbtion at 280nm? - (reply: 1)
477. Buttery Popcorn Odor in Contaminated Expression Prep - (reply: 3)
478. Self-made Protease inhibitor cocktail - PMSF, EDTA, Benzamidine (reply: 4)
479. Contamination in GST fusion protein purification - (reply: 2)
480. GST-tagged proteins - (reply: 1)

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