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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
451. How much protein should be loaded to the column during the purificiation? - Is less more? ;) (reply: 1)
452. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
453. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
454. Centrifugal Filter Unit - Trying to get a higher concentration. (reply: 3)
455. IMPACT system purification problem - (reply: 1)
456. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
457. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
458. How to normalize my samples before I transfect my cells - (reply: 6)
459. FBS interference in SDS PAGE - (reply: 3)
460. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
461. What make voltage not going up for the 3rd step in running IEF? - (reply: 2)
462. Help...purify and concentrate protein for IEF - (reply: 1)
463. preference of N/C terminus for tagging - (reply: 2)
464. NI-NTA purification - (reply: 2)
465. Could a 30aa peptides(3×flag+6×his) be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)
466. Ampicillin Mess-up(Experiment in progress atm) - Experiment still valid? (reply: 1)
467. RNA immunoprecipitation...HELP! - (reply: 4)
468. Dialysis - A question about buffers (reply: 6)
469. Purification problems his-tag - I have tried everything (reply: 3)
470. GST-tagged protein binds to column but is not eluted - (reply: 1)
471. urea-denatured protein precipitates after dialysis - (reply: 1)
472. Expressing 4 proteins in an operon - (reply: 6)
473. More His-tag problems - (reply: 4)
474. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
475. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
476. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
477. Protein precipitates upon dialysis - (reply: 5)
478. membrane protein aggregation - (reply: 1)
479. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
480. His Tag Protein Purification - (reply: 6)

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