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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
451. Removal of Tightly Bound Nucleic Acids - (reply: 3)
452. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
453. Recombinant protein aggregation after purification - (reply: 2)
454. average total protein content per 293t cell - (reply: 2)
455. Problems with Preparative Isoelectrofocusing (minirotofor) - (reply: 1)
456. expression of MBP fusion protein - leaky and very low yield (reply: 2)
457. Calmodulin binding assay - (reply: 1)
458. strange problem with His purification - (reply: 1)
459. E.coli expression - (reply: 5)
460. why my protein precipitates? - (reply: 2)
461. DTT in GST elution buffer - (reply: 1)
462. vectors and his-tags - (reply: 3)
463. SDS boiled samples - (reply: 1)
464. E coli lysis by lysozyme - (reply: 2)
465. His-tag protein expression - (reply: 1)
466. SDS-PAGE loading dye - (reply: 1)
467. How to concentrate the protein - (reply: 4)
468. protein overexpression - factors affecting overexpression (reply: 3)
469. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
470. e coli strain selection for protein expression - (reply: 2)
471. Problem clariflying/filtering insect cell lysate - (reply: 2)
472. Ni column changing colors - (reply: 2)
473. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
474. Problem with Histag purification - (reply: 1)
475. Freezing eluted His tag protein - (reply: 5)
476. Why Ni-NTA beads became clumped after incubation? - Intensive Clumped (reply: 2)
477. His-tag protein lost it's tag - (reply: 2)
478. AnionExchange problem: proteins do not bind - (reply: 12)
479. How much protein should be loaded to the column during the purificiation? - Is less more? ;) (reply: 1)
480. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)

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