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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
451. DTT in GST elution buffer - (reply: 1)
452. vectors and his-tags - (reply: 3)
453. SDS boiled samples - (reply: 1)
454. E coli lysis by lysozyme - (reply: 2)
455. His-tag protein expression - (reply: 1)
456. SDS-PAGE loading dye - (reply: 1)
457. How to concentrate the protein - (reply: 4)
458. protein overexpression - factors affecting overexpression (reply: 3)
459. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
460. e coli strain selection for protein expression - (reply: 2)
461. Problem clariflying/filtering insect cell lysate - (reply: 2)
462. Ni column changing colors - (reply: 2)
463. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
464. Problem with Histag purification - (reply: 1)
465. Freezing eluted His tag protein - (reply: 5)
466. Why Ni-NTA beads became clumped after incubation? - Intensive Clumped (reply: 2)
467. His-tag protein lost it's tag - (reply: 2)
468. AnionExchange problem: proteins do not bind - (reply: 12)
469. How much protein should be loaded to the column during the purificiation? - Is less more? ;) (reply: 1)
470. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
471. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
472. Centrifugal Filter Unit - Trying to get a higher concentration. (reply: 3)
473. IMPACT system purification problem - (reply: 1)
474. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
475. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
476. How to normalize my samples before I transfect my cells - (reply: 6)
477. FBS interference in SDS PAGE - (reply: 3)
478. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
479. What make voltage not going up for the 3rd step in running IEF? - (reply: 2)
480. Help...purify and concentrate protein for IEF - (reply: 1)

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