Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
451. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
452. Recombinant protein aggregation after purification - (reply: 2)
453. average total protein content per 293t cell - (reply: 2)
454. Problems with Preparative Isoelectrofocusing (minirotofor) - (reply: 1)
455. expression of MBP fusion protein - leaky and very low yield (reply: 2)
456. Calmodulin binding assay - (reply: 1)
457. strange problem with His purification - (reply: 1)
458. E.coli expression - (reply: 5)
459. why my protein precipitates? - (reply: 2)
460. DTT in GST elution buffer - (reply: 1)
461. vectors and his-tags - (reply: 3)
462. SDS boiled samples - (reply: 1)
463. E coli lysis by lysozyme - (reply: 2)
464. His-tag protein expression - (reply: 1)
465. SDS-PAGE loading dye - (reply: 1)
466. How to concentrate the protein - (reply: 4)
467. protein overexpression - factors affecting overexpression (reply: 3)
468. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
469. e coli strain selection for protein expression - (reply: 2)
470. Problem clariflying/filtering insect cell lysate - (reply: 2)
471. Ni column changing colors - (reply: 2)
472. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
473. Problem with Histag purification - (reply: 1)
474. Freezing eluted His tag protein - (reply: 5)
475. Why Ni-NTA beads became clumped after incubation? - Intensive Clumped (reply: 2)
476. His-tag protein lost it's tag - (reply: 2)
477. AnionExchange problem: proteins do not bind - (reply: 12)
478. How much protein should be loaded to the column during the purificiation? - Is less more? ;) (reply: 1)
479. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
480. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22