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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
451. Trouble With HIS affinity column (Ni+) binding - (reply: 1)
452. Problems with affinity purification with M2 beads - (reply: 2)
453. Expression problem using invitrogen pRSET C vector - (reply: 5)
454. Vector containing N-his tag and TEV - (reply: 1)
455. Protein expression using invitrogen pSecTag2 - (reply: 2)
456. Problems with nuclear extraction - (reply: 1)
457. His-tagged protein runs much faster than expected - 6His-Tagged CcpA protein runs around 26 kD whereas I expect to run 37 (reply: 7)
458. Removing phosphates - (reply: 2)
459. protein going to inclusion bodies - protein going to inclusion bodies (reply: 1)
460. Looking for endogenus expression by using custom antibody - (reply: 5)
461. Adventitious chemical binding - Unwanted chemicals in protein solution (reply: 2)
462. More than one protein induced - (reply: 4)
463. Protein aggregates - Monomeric fraction varies with wavelength (reply: 1)
464. water withdrawal with PEG20.000 - Does it drag glycerol along too?-PLEASE HELP! (reply: 1)
465. Troubleshooting GST purification - (reply: 4)
466. Amount of inoculum? - for protein expression in E.coli (reply: 3)
467. BL21A or simliar - (reply: 2)
468. Role of imidazole in lysis buffer - for obtaining recombinant his-tag protein (reply: 4)
469. protein expression (temperature issue) - trying to express soluble protein at lower temperature (reply: 17)
470. Can GST-tag be separated from GST-fusion protein - (reply: 1)
471. Protein analog - (reply: 1)
472. membrane protein extraction buffer - (reply: 1)
473. What about the Baculovirus expression system? - (reply: 2)
474. Ni-NTA beads and dialysis tubing - Purification using nickel beads (reply: 4)
475. Concentrating Protein with centrifuge concentrator but 50% glycerol!!? - (reply: 2)
476. protein is degraded after purification - (reply: 2)
477. protein expression - Preparation of starter cultures (reply: 15)
478. Problem with Protein elution using GST sepharose 4B - (reply: 1)
479. how to isolate insoluble protein in bacterial induction? - (reply: 4)
480. Plasmid instability in E.coli BL21? - (reply: 3)

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