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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
451. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
452. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
453. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
454. Protein precipitates upon dialysis - (reply: 5)
455. membrane protein aggregation - (reply: 1)
456. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
457. His Tag Protein Purification - (reply: 6)
458. Amylose bound protein elution - (reply: 1)
459. hey guys,what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
460. Soluble aggregates - (reply: 3)
461. insect protein expression - (reply: 1)
462. Can you store TNT translation products? - (reply: 1)
463. How to pack the FPLC column - (reply: 2)
464. Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
465. Sonication issues! - (reply: 3)
466. complete gene for expression? - (reply: 2)
467. buffer for membrane protein - (reply: 1)
468. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
469. Question: Bradford assay - buffer precipitates - KCl and HEPES preciptation with Bradford reagent (reply: 3)
470. membrane protein purification - (reply: 6)
471. Protein expression failing - (reply: 1)
472. How does aggregation influence absorbtion at 280nm? - (reply: 1)
473. Buttery Popcorn Odor in Contaminated Expression Prep - (reply: 3)
474. Self-made Protease inhibitor cocktail - PMSF, EDTA, Benzamidine (reply: 4)
475. Contamination in GST fusion protein purification - (reply: 2)
476. GST-tagged proteins - (reply: 1)
477. Protino® Ni-IDA Packed Columns - Can you re-use them? (reply: 1)
478. immunoprecipation - (reply: 1)
479. Ni-NTA his tag protein purification - (reply: 1)
480. Weird Induction Problem - (reply: 5)

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