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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
451. Buttery Popcorn Odor in Contaminated Expression Prep - (reply: 3)
452. Self-made Protease inhibitor cocktail - PMSF, EDTA, Benzamidine (reply: 4)
453. Contamination in GST fusion protein purification - (reply: 2)
454. GST-tagged proteins - (reply: 1)
455. Protino® Ni-IDA Packed Columns - Can you re-use them? (reply: 1)
456. immunoprecipation - (reply: 1)
457. Ni-NTA his tag protein purification - (reply: 1)
458. Weird Induction Problem - (reply: 5)
459. cloning of non-coding region for protein expression - protein expression (reply: 1)
460. from pet-SUMO to pet-28 - Expression problems with pet SUMO (reply: 2)
461. Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
462. Protein expression - (reply: 4)
463. EDTA bacterial cell lysate - (reply: 2)
464. Trouble With HIS affinity column (Ni+) binding - (reply: 1)
465. Problems with affinity purification with M2 beads - (reply: 2)
466. Expression problem using invitrogen pRSET C vector - (reply: 5)
467. Vector containing N-his tag and TEV - (reply: 1)
468. Protein expression using invitrogen pSecTag2 - (reply: 2)
469. Problems with nuclear extraction - (reply: 1)
470. His-tagged protein runs much faster than expected - 6His-Tagged CcpA protein runs around 26 kD whereas I expect to run 37 (reply: 7)
471. Removing phosphates - (reply: 2)
472. protein going to inclusion bodies - protein going to inclusion bodies (reply: 1)
473. Looking for endogenus expression by using custom antibody - (reply: 5)
474. Adventitious chemical binding - Unwanted chemicals in protein solution (reply: 2)
475. More than one protein induced - (reply: 4)
476. Protein aggregates - Monomeric fraction varies with wavelength (reply: 1)
477. water withdrawal with PEG20.000 - Does it drag glycerol along too?-PLEASE HELP! (reply: 1)
478. Troubleshooting GST purification - (reply: 4)
479. Amount of inoculum? - for protein expression in E.coli (reply: 3)
480. BL21A or simliar - (reply: 2)

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