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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
451. protein overexpression - factors affecting overexpression (reply: 3)
452. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
453. e coli strain selection for protein expression - (reply: 2)
454. Problem clariflying/filtering insect cell lysate - (reply: 2)
455. Ni column changing colors - (reply: 2)
456. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
457. Problem with Histag purification - (reply: 1)
458. Freezing eluted His tag protein - (reply: 5)
459. Why Ni-NTA beads became clumped after incubation? - Intensive Clumped (reply: 2)
460. His-tag protein lost it's tag - (reply: 2)
461. AnionExchange problem: proteins do not bind - (reply: 12)
462. How much protein should be loaded to the column during the purificiation? - Is less more? ;) (reply: 1)
463. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
464. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
465. Centrifugal Filter Unit - Trying to get a higher concentration. (reply: 3)
466. IMPACT system purification problem - (reply: 1)
467. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
468. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
469. How to normalize my samples before I transfect my cells - (reply: 6)
470. FBS interference in SDS PAGE - (reply: 3)
471. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
472. What make voltage not going up for the 3rd step in running IEF? - (reply: 2)
473. Help...purify and concentrate protein for IEF - (reply: 1)
474. preference of N/C terminus for tagging - (reply: 2)
475. NI-NTA purification - (reply: 2)
476. Could a 30aa peptides(3×flag+6×his) be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)
477. Ampicillin Mess-up(Experiment in progress atm) - Experiment still valid? (reply: 1)
478. RNA immunoprecipitation...HELP! - (reply: 4)
479. Dialysis - A question about buffers (reply: 6)
480. Purification problems his-tag - I have tried everything (reply: 3)

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