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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
451. Purification using Low-Salt Lysis Buffer & Downstream app. - (reply: 2)
452. M2 Flag purification - (reply: 2)
453. Quantitating IP'd proteins - (reply: 1)
454. Removal of Tightly Bound Nucleic Acids - (reply: 3)
455. Why there are so many plasmid in Chaperone competent cell set? - (reply: 1)
456. Recombinant protein aggregation after purification - (reply: 2)
457. average total protein content per 293t cell - (reply: 2)
458. Problems with Preparative Isoelectrofocusing (minirotofor) - (reply: 1)
459. expression of MBP fusion protein - leaky and very low yield (reply: 2)
460. Calmodulin binding assay - (reply: 1)
461. strange problem with His purification - (reply: 1)
462. E.coli expression - (reply: 5)
463. why my protein precipitates? - (reply: 2)
464. DTT in GST elution buffer - (reply: 1)
465. vectors and his-tags - (reply: 3)
466. SDS boiled samples - (reply: 1)
467. E coli lysis by lysozyme - (reply: 2)
468. His-tag protein expression - (reply: 1)
469. SDS-PAGE loading dye - (reply: 1)
470. How to concentrate the protein - (reply: 4)
471. protein overexpression - factors affecting overexpression (reply: 3)
472. Expression in E.coli - only 1 out of 7 constructs working! (reply: 16)
473. e coli strain selection for protein expression - (reply: 2)
474. Problem clariflying/filtering insect cell lysate - (reply: 2)
475. Ni column changing colors - (reply: 2)
476. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
477. Problem with Histag purification - (reply: 1)
478. Freezing eluted His tag protein - (reply: 5)
479. Why Ni-NTA beads became clumped after incubation? - Intensive Clumped (reply: 2)
480. His-tag protein lost it's tag - (reply: 2)

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