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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
31. Bradford positive but no Protein on SDS PAGE - (reply: 4)
32. Disadvantage of crossflow filtration - (reply: 3)
33. Help with Superdex200 increase Column - (reply: 1)
34. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
35. Loss of protein expression by transformed bacteria - (reply: 4)
36. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
37. after protein purification - (reply: 4)
38. Tryple express vs scraping - (reply: 2)
39. Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? - (reply: 1)
40. Predicting amount maximum of sample to apply onto cation exchange chromatography - (reply: 5)
41. recombinant protein vs native protein - (reply: 1)
42. protein expression site - (reply: 3)
43. Beads pull down only free GST - (reply: 14)
44. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
45. Protein Expresion and Isolation from Lactobacillus - Insoluble protein - (reply: 1)
46. protein expression - (reply: 2)
47. Anion Exchange Buffers - (reply: 2)
48. Difficulty eluting His-tag Zn metalloprotease - (reply: 1)
49. about sds-page buffers - (reply: 1)
50. How do I extract soluble protein after adding SDS-PAGE buffer? - (reply: 1)
51. Best epitope for IF with a poor-expresser - (reply: 2)
52. Collagen binding protein expression E. coli His-tag - (reply: 1)
53. pet-32 - (reply: 3)
54. Elute fractions that have target protein in FPLC is unstable - (reply: 8)
55. How can i enrich highly -ve charge biomolecules using High Q resin? - (reply: 1)
56. Question about MBP purification - (reply: 2)
57. unexpected protein bands - (reply: 2)
58. Urea effect on mice spleen cells - (reply: 1)
59. Non-pH-sensitive fluorophore for relative protein quantification - (reply: 1)
60. Validating Recombinant Protein - (reply: 1)

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