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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
31. Problem with calculations for enzyme activity - (reply: 2)
32. problem with sds-page - (reply: 8)
33. Ammonium sulfate fractionalization help - (reply: 1)
34. heme protein precipitation - (reply: 4)
35. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 4)
36. regarding protein purification using 8m urea and sds page - (reply: 2)
37. how do you inactivate His-tag affinity beads?? - (reply: 4)
38. Protein Purification from mammalian cells - (reply: 1)
39. DNA cleanup after linearization, before transformation? - (reply: 3)
40. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
41. predict VLP formation - (reply: 1)
42. need help for transfection to synchronized cells - (reply: 4)
43. Pulldown of polyubiquitinated proteins - (reply: 1)
44. membrane protein expression - (reply: 2)
45. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
46. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
47. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
48. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
49. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
50. protein extraction from cell nucleus - (reply: 2)
51. his-tag purification - (reply: 9)
52. selection of the media for ion exchange chromatography - (reply: 1)
53. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
54. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
55. Does a C-terminal Proline Residue Increase Degradation Risk? - (reply: 2)
56. His-tag and GST-tag - (reply: 1)
57. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
58. Do protein purification under partially denatured condition - (reply: 1)
59. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
60. how to remove affinity tag completely - (reply: 11)

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