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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
31. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
32. genotype of e coli strain for protein expression - (reply: 2)
33. Problems with detection of protein expressed in yeast - (reply: 1)
34. thionin - (reply: 1)
35. How do I go about making a set of protein standards for protein quantification v - (reply: 2)
36. secretome and zymograms - (reply: 1)
37. Protein Refolding - Precipitation during dialysis - (reply: 3)
38. Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh - (reply: 1)
39. Problem with calculations for enzyme activity - (reply: 2)
40. problem with sds-page - (reply: 8)
41. Ammonium sulfate fractionalization help - (reply: 1)
42. heme protein precipitation - (reply: 4)
43. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 4)
44. regarding protein purification using 8m urea and sds page - (reply: 2)
45. how do you inactivate His-tag affinity beads?? - (reply: 4)
46. Protein Purification from mammalian cells - (reply: 1)
47. DNA cleanup after linearization, before transformation? - (reply: 3)
48. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
49. predict VLP formation - (reply: 1)
50. need help for transfection to synchronized cells - (reply: 4)
51. Pulldown of polyubiquitinated proteins - (reply: 1)
52. membrane protein expression - (reply: 2)
53. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
54. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
55. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
56. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
57. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
58. protein extraction from cell nucleus - (reply: 2)
59. his-tag purification - (reply: 9)
60. selection of the media for ion exchange chromatography - (reply: 1)

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