Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
31. lysate protein concentration - (reply: 1)
32. Cloned gene in pET system not being expressed - (reply: 10)
33. recombinant protein expression- surface or internal? - (reply: 3)
34. His-tag protein not binding to resin - (reply: 9)
35. Protein localisation - IHC - (reply: 1)
36. Collagen Tipe I filtration - (reply: 1)
37. Removal of cleaved peptide after Protease cleavage - (reply: 4)
38. Bradford positive but no Protein on SDS PAGE - (reply: 4)
39. Disadvantage of crossflow filtration - (reply: 3)
40. Help with Superdex200 increase Column - (reply: 1)
41. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
42. Loss of protein expression by transformed bacteria - (reply: 4)
43. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
44. after protein purification - (reply: 4)
45. Tryple express vs scraping - (reply: 2)
46. Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? - (reply: 1)
47. Predicting amount maximum of sample to apply onto cation exchange chromatography - (reply: 5)
48. recombinant protein vs native protein - (reply: 1)
49. protein expression site - (reply: 3)
50. Beads pull down only free GST - (reply: 14)
51. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
52. Protein Expresion and Isolation from Lactobacillus - Insoluble protein - (reply: 1)
53. protein expression - (reply: 2)
54. Anion Exchange Buffers - (reply: 2)
55. Difficulty eluting His-tag Zn metalloprotease - (reply: 1)
56. about sds-page buffers - (reply: 1)
57. How do I extract soluble protein after adding SDS-PAGE buffer? - (reply: 1)
58. Best epitope for IF with a poor-expresser - (reply: 2)
59. Collagen binding protein expression E. coli His-tag - (reply: 1)
60. pet-32 - (reply: 3)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22