Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
31. Does over-expression protein under T7 promoter in Top10 work? - (reply: 1)
32. Large-scale cell growth and protein expression - (reply: 1)
33. Reduce protein concentration without reduced enzyme activity - (reply: 3)
34. consistent expression of overlarge proteins - (reply: 1)
35. Cytoplasmic/nuclear fractionation with NP-40 then RIPA buffer combination? - (reply: 2)
36. Is AS pp + HIC phisically possible for all proteins? - (reply: 2)
37. storage conditions for purified protein - (reply: 2)
38. Need to include protease inhibitor in IP wash buffer? - (reply: 1)
39. Heat shock vs eletroporation - (reply: 6)
40. lysate protein concentration - (reply: 1)
41. Cloned gene in pET system not being expressed - (reply: 10)
42. recombinant protein expression- surface or internal? - (reply: 3)
43. His-tag protein not binding to resin - (reply: 9)
44. Protein localisation - IHC - (reply: 1)
45. Collagen Tipe I filtration - (reply: 1)
46. Removal of cleaved peptide after Protease cleavage - (reply: 4)
47. Bradford positive but no Protein on SDS PAGE - (reply: 4)
48. Disadvantage of crossflow filtration - (reply: 3)
49. Help with Superdex200 increase Column - (reply: 1)
50. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
51. Loss of protein expression by transformed bacteria - (reply: 4)
52. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
53. after protein purification - (reply: 4)
54. Tryple express vs scraping - (reply: 2)
55. Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? - (reply: 1)
56. Predicting amount maximum of sample to apply onto cation exchange chromatography - (reply: 5)
57. recombinant protein vs native protein - (reply: 1)
58. protein expression site - (reply: 3)
59. Beads pull down only free GST - (reply: 14)
60. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22