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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
31. Problems with a nonspecific interaction on a Ni-NTA column - (reply: 3)
32. protein expression with and without induction - (reply: 1)
33. Please help me :Protein expression and purification - (reply: 1)
34. Troubleshooting: His-Tag Nickle Column Purification - (reply: 1)
35. genotype of e coli strain for protein expression - (reply: 2)
36. Problems with detection of protein expressed in yeast - (reply: 1)
37. thionin - (reply: 1)
38. How do I go about making a set of protein standards for protein quantification v - (reply: 2)
39. secretome and zymograms - (reply: 1)
40. Protein Refolding - Precipitation during dialysis - (reply: 3)
41. Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh - (reply: 1)
42. Problem with calculations for enzyme activity - (reply: 2)
43. problem with sds-page - (reply: 8)
44. Ammonium sulfate fractionalization help - (reply: 1)
45. heme protein precipitation - (reply: 4)
46. Transient transfection using linear 25 kDa PEI transfection reagent in CHO k1 - (reply: 4)
47. regarding protein purification using 8m urea and sds page - (reply: 2)
48. how do you inactivate His-tag affinity beads?? - (reply: 4)
49. Protein Purification from mammalian cells - (reply: 1)
50. DNA cleanup after linearization, before transformation? - (reply: 3)
51. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
52. predict VLP formation - (reply: 1)
53. need help for transfection to synchronized cells - (reply: 4)
54. Pulldown of polyubiquitinated proteins - (reply: 1)
55. membrane protein expression - (reply: 2)
56. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
57. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
58. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
59. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
60. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)

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