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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
31. storage conditions for purified protein - (reply: 2)
32. Need to include protease inhibitor in IP wash buffer? - (reply: 1)
33. Heat shock vs eletroporation - (reply: 6)
34. lysate protein concentration - (reply: 1)
35. Cloned gene in pET system not being expressed - (reply: 10)
36. recombinant protein expression- surface or internal? - (reply: 3)
37. His-tag protein not binding to resin - (reply: 9)
38. Protein localisation - IHC - (reply: 1)
39. Collagen Tipe I filtration - (reply: 1)
40. Removal of cleaved peptide after Protease cleavage - (reply: 4)
41. Bradford positive but no Protein on SDS PAGE - (reply: 4)
42. Disadvantage of crossflow filtration - (reply: 3)
43. Help with Superdex200 increase Column - (reply: 1)
44. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
45. Loss of protein expression by transformed bacteria - (reply: 4)
46. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
47. after protein purification - (reply: 4)
48. Tryple express vs scraping - (reply: 2)
49. Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? - (reply: 1)
50. Predicting amount maximum of sample to apply onto cation exchange chromatography - (reply: 5)
51. recombinant protein vs native protein - (reply: 1)
52. protein expression site - (reply: 3)
53. Beads pull down only free GST - (reply: 14)
54. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
55. Protein Expresion and Isolation from Lactobacillus - Insoluble protein - (reply: 1)
56. protein expression - (reply: 2)
57. Anion Exchange Buffers - (reply: 2)
58. Difficulty eluting His-tag Zn metalloprotease - (reply: 1)
59. about sds-page buffers - (reply: 1)
60. How do I extract soluble protein after adding SDS-PAGE buffer? - (reply: 1)

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