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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
31. protein expression site - (reply: 3)
32. Beads pull down only free GST - (reply: 14)
33. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
34. Protein Expresion and Isolation from Lactobacillus - Insoluble protein - (reply: 1)
35. protein expression - (reply: 2)
36. Anion Exchange Buffers - (reply: 2)
37. Difficulty eluting His-tag Zn metalloprotease - (reply: 1)
38. about sds-page buffers - (reply: 1)
39. How do I extract soluble protein after adding SDS-PAGE buffer? - (reply: 1)
40. Best epitope for IF with a poor-expresser - (reply: 2)
41. Collagen binding protein expression E. coli His-tag - (reply: 1)
42. pet-32 - (reply: 3)
43. Elute fractions that have target protein in FPLC is unstable - (reply: 8)
44. How can i enrich highly -ve charge biomolecules using High Q resin? - (reply: 1)
45. Question about MBP purification - (reply: 2)
46. unexpected protein bands - (reply: 2)
47. Urea effect on mice spleen cells - (reply: 1)
48. Non-pH-sensitive fluorophore for relative protein quantification - (reply: 1)
49. Validating Recombinant Protein - (reply: 1)
50. Sonication of cells in ultrasound bath, without probe - (reply: 3)
51. Proteinase extraction and purification - (reply: 1)
52. E.Coli Lysis: To add Lysozyme or not to add Lysozyme, that is the question - (reply: 2)
53. EGFP expression - (reply: 1)
54. the same construct, two stable cell clones with different protein size - (reply: 2)
55. Purification of metallothioneins? - (reply: 1)
56. RIPA buffer for IP and western - (reply: 1)
57. i am trying to get rid of unexpected protein band by centricon - (reply: 3)
58. CO- IP for nuclear protein - (reply: 4)
59. Trichloroacetic Acid precipitation of proteins - (reply: 2)
60. Problems with a nonspecific interaction on a Ni-NTA column - (reply: 3)

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