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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
31. Determining Cofactor for Inactive Enzyme - (reply: 6)
32. study protein expression in bacteria - (reply: 1)
33. total protein measurement from insect body - (reply: 1)
34. Does over-expression protein under T7 promoter in Top10 work? - (reply: 1)
35. Large-scale cell growth and protein expression - (reply: 1)
36. Reduce protein concentration without reduced enzyme activity - (reply: 3)
37. consistent expression of overlarge proteins - (reply: 1)
38. Cytoplasmic/nuclear fractionation with NP-40 then RIPA buffer combination? - (reply: 2)
39. Is AS pp + HIC phisically possible for all proteins? - (reply: 2)
40. storage conditions for purified protein - (reply: 2)
41. Need to include protease inhibitor in IP wash buffer? - (reply: 1)
42. Heat shock vs eletroporation - (reply: 6)
43. lysate protein concentration - (reply: 1)
44. Cloned gene in pET system not being expressed - (reply: 10)
45. recombinant protein expression- surface or internal? - (reply: 3)
46. His-tag protein not binding to resin - (reply: 9)
47. Protein localisation - IHC - (reply: 1)
48. Collagen Tipe I filtration - (reply: 1)
49. Removal of cleaved peptide after Protease cleavage - (reply: 4)
50. Bradford positive but no Protein on SDS PAGE - (reply: 4)
51. Disadvantage of crossflow filtration - (reply: 3)
52. Help with Superdex200 increase Column - (reply: 1)
53. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
54. Loss of protein expression by transformed bacteria - (reply: 4)
55. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
56. after protein purification - (reply: 4)
57. Tryple express vs scraping - (reply: 2)
58. Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? - (reply: 1)
59. Predicting amount maximum of sample to apply onto cation exchange chromatography - (reply: 5)
60. recombinant protein vs native protein - (reply: 1)

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