Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
31. His-tag protein not binding to resin - (reply: 9)
32. Protein localisation - IHC - (reply: 1)
33. Collagen Tipe I filtration - (reply: 1)
34. Removal of cleaved peptide after Protease cleavage - (reply: 4)
35. Bradford positive but no Protein on SDS PAGE - (reply: 4)
36. Disadvantage of crossflow filtration - (reply: 3)
37. Help with Superdex200 increase Column - (reply: 1)
38. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
39. Loss of protein expression by transformed bacteria - (reply: 4)
40. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
41. after protein purification - (reply: 4)
42. Tryple express vs scraping - (reply: 2)
43. Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? - (reply: 1)
44. Predicting amount maximum of sample to apply onto cation exchange chromatography - (reply: 5)
45. recombinant protein vs native protein - (reply: 1)
46. protein expression site - (reply: 3)
47. Beads pull down only free GST - (reply: 14)
48. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
49. Protein Expresion and Isolation from Lactobacillus - Insoluble protein - (reply: 1)
50. protein expression - (reply: 2)
51. Anion Exchange Buffers - (reply: 2)
52. Difficulty eluting His-tag Zn metalloprotease - (reply: 1)
53. about sds-page buffers - (reply: 1)
54. How do I extract soluble protein after adding SDS-PAGE buffer? - (reply: 1)
55. Best epitope for IF with a poor-expresser - (reply: 2)
56. Collagen binding protein expression E. coli His-tag - (reply: 1)
57. pet-32 - (reply: 3)
58. Elute fractions that have target protein in FPLC is unstable - (reply: 8)
59. How can i enrich highly -ve charge biomolecules using High Q resin? - (reply: 1)
60. Question about MBP purification - (reply: 2)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22