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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
31. regarding protein purification using 8m urea and sds page - (reply: 2)
32. how do you inactivate His-tag affinity beads?? - (reply: 4)
33. Protein Purification from mammalian cells - (reply: 1)
34. DNA cleanup after linearization, before transformation? - (reply: 3)
35. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
36. predict VLP formation - (reply: 1)
37. need help for transfection to synchronized cells - (reply: 4)
38. Pulldown of polyubiquitinated proteins - (reply: 1)
39. membrane protein expression - (reply: 2)
40. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
41. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
42. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
43. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
44. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
45. protein extraction from cell nucleus - (reply: 2)
46. his-tag purification - (reply: 9)
47. selection of the media for ion exchange chromatography - (reply: 1)
48. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
49. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
50. Does a C-terminal Proline Residue Increase Degradation Risk? - (reply: 2)
51. His-tag and GST-tag - (reply: 1)
52. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
53. Do protein purification under partially denatured condition - (reply: 1)
54. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
55. how to remove affinity tag completely - (reply: 11)
56. Agarose bead size in affinity chromatography - (reply: 2)
57. Protein precipitation by storage - (reply: 11)
58. designing fusion protein with Flag tag - (reply: 1)
59. Problems with biotynylated protein binding to monomeric avidin - (reply: 1)
60. Control protein for eukaryotic expression - (reply: 5)

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