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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
31. Removal of cleaved peptide after Protease cleavage - (reply: 4)
32. Bradford positive but no Protein on SDS PAGE - (reply: 4)
33. Disadvantage of crossflow filtration - (reply: 3)
34. Help with Superdex200 increase Column - (reply: 1)
35. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
36. Loss of protein expression by transformed bacteria - (reply: 4)
37. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
38. after protein purification - (reply: 4)
39. Tryple express vs scraping - (reply: 2)
40. Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? - (reply: 1)
41. Predicting amount maximum of sample to apply onto cation exchange chromatography - (reply: 5)
42. recombinant protein vs native protein - (reply: 1)
43. protein expression site - (reply: 3)
44. Beads pull down only free GST - (reply: 14)
45. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
46. Protein Expresion and Isolation from Lactobacillus - Insoluble protein - (reply: 1)
47. protein expression - (reply: 2)
48. Anion Exchange Buffers - (reply: 2)
49. Difficulty eluting His-tag Zn metalloprotease - (reply: 1)
50. about sds-page buffers - (reply: 1)
51. How do I extract soluble protein after adding SDS-PAGE buffer? - (reply: 1)
52. Best epitope for IF with a poor-expresser - (reply: 2)
53. Collagen binding protein expression E. coli His-tag - (reply: 1)
54. pet-32 - (reply: 3)
55. Elute fractions that have target protein in FPLC is unstable - (reply: 8)
56. How can i enrich highly -ve charge biomolecules using High Q resin? - (reply: 1)
57. Question about MBP purification - (reply: 2)
58. unexpected protein bands - (reply: 2)
59. Urea effect on mice spleen cells - (reply: 1)
60. Non-pH-sensitive fluorophore for relative protein quantification - (reply: 1)

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