Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
31. recombinant protein expression- surface or internal? - (reply: 3)
32. His-tag protein not binding to resin - (reply: 9)
33. Protein localisation - IHC - (reply: 1)
34. Collagen Tipe I filtration - (reply: 1)
35. Removal of cleaved peptide after Protease cleavage - (reply: 4)
36. Bradford positive but no Protein on SDS PAGE - (reply: 4)
37. Disadvantage of crossflow filtration - (reply: 3)
38. Help with Superdex200 increase Column - (reply: 1)
39. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
40. Loss of protein expression by transformed bacteria - (reply: 4)
41. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
42. after protein purification - (reply: 4)
43. Tryple express vs scraping - (reply: 2)
44. Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? - (reply: 1)
45. Predicting amount maximum of sample to apply onto cation exchange chromatography - (reply: 5)
46. recombinant protein vs native protein - (reply: 1)
47. protein expression site - (reply: 3)
48. Beads pull down only free GST - (reply: 14)
49. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
50. Protein Expresion and Isolation from Lactobacillus - Insoluble protein - (reply: 1)
51. protein expression - (reply: 2)
52. Anion Exchange Buffers - (reply: 2)
53. Difficulty eluting His-tag Zn metalloprotease - (reply: 1)
54. about sds-page buffers - (reply: 1)
55. How do I extract soluble protein after adding SDS-PAGE buffer? - (reply: 1)
56. Best epitope for IF with a poor-expresser - (reply: 2)
57. Collagen binding protein expression E. coli His-tag - (reply: 1)
58. pet-32 - (reply: 3)
59. Elute fractions that have target protein in FPLC is unstable - (reply: 8)
60. How can i enrich highly -ve charge biomolecules using High Q resin? - (reply: 1)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22