Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
31. DNA cleanup after linearization, before transformation? - (reply: 3)
32. Can I use cellufine sulfate cloumn as a strong cation exchange? - (reply: 2)
33. predict VLP formation - (reply: 1)
34. need help for transfection to synchronized cells - (reply: 4)
35. Pulldown of polyubiquitinated proteins - (reply: 1)
36. membrane protein expression - (reply: 2)
37. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
38. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
39. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
40. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
41. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
42. protein extraction from cell nucleus - (reply: 2)
43. his-tag purification - (reply: 9)
44. selection of the media for ion exchange chromatography - (reply: 1)
45. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
46. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
47. Does a C-terminal Proline Residue Increase Degradation Risk? - (reply: 2)
48. His-tag and GST-tag - (reply: 1)
49. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
50. Do protein purification under partially denatured condition - (reply: 1)
51. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
52. how to remove affinity tag completely - (reply: 11)
53. Agarose bead size in affinity chromatography - (reply: 2)
54. Protein precipitation by storage - (reply: 11)
55. designing fusion protein with Flag tag - (reply: 1)
56. Problems with biotynylated protein binding to monomeric avidin - (reply: 1)
57. Control protein for eukaryotic expression - (reply: 5)
58. Flag fusion protein not detected by Flag antibody - (reply: 8)
59. E. coli culture - (reply: 1)
60. instability index of expressed protein - (reply: 1)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20