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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
31. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
32. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
33. protein extraction from cell nucleus - (reply: 2)
34. his-tag purification - (reply: 9)
35. selection of the media for ion exchange chromatography - (reply: 1)
36. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
37. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
38. Does a C-terminal Proline Residue Increase Degradation Risk? - (reply: 2)
39. His-tag and GST-tag - (reply: 1)
40. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
41. Do protein purification under partially denatured condition - (reply: 1)
42. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
43. how to remove affinity tag completely - (reply: 11)
44. Agarose bead size in affinity chromatography - (reply: 2)
45. Protein precipitation by storage - (reply: 11)
46. designing fusion protein with Flag tag - (reply: 1)
47. Problems with biotynylated protein binding to monomeric avidin - (reply: 1)
48. Control protein for eukaryotic expression - (reply: 5)
49. Flag fusion protein not detected by Flag antibody - (reply: 8)
50. E. coli culture - (reply: 1)
51. instability index of expressed protein - (reply: 1)
52. Protein Purification-catastrophe while purifying? - (reply: 2)
53. T5 Promoter - (reply: 1)
54. Protein purification for adherent mammalian cells - (reply: 2)
55. Antibody production in Expi293 cells - (reply: 2)
56. pGEX-2t purification problem - (reply: 3)
57. Phosphorylation of recombinant proteins in E. coli - (reply: 1)
58. Compatibility of Urea and Imidazole - (reply: 2)
59. Growth media - OD at induction - growth phases... - (reply: 2)
60. NusA tag produces protein truncations? - (reply: 3)

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