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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
31. Is AS pp + HIC phisically possible for all proteins? - (reply: 2)
32. storage conditions for purified protein - (reply: 2)
33. Need to include protease inhibitor in IP wash buffer? - (reply: 1)
34. Heat shock vs eletroporation - (reply: 6)
35. lysate protein concentration - (reply: 1)
36. Cloned gene in pET system not being expressed - (reply: 10)
37. recombinant protein expression- surface or internal? - (reply: 3)
38. His-tag protein not binding to resin - (reply: 9)
39. Protein localisation - IHC - (reply: 1)
40. Collagen Tipe I filtration - (reply: 1)
41. Removal of cleaved peptide after Protease cleavage - (reply: 4)
42. Bradford positive but no Protein on SDS PAGE - (reply: 4)
43. Disadvantage of crossflow filtration - (reply: 3)
44. Help with Superdex200 increase Column - (reply: 1)
45. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
46. Loss of protein expression by transformed bacteria - (reply: 4)
47. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
48. after protein purification - (reply: 4)
49. Tryple express vs scraping - (reply: 2)
50. Pierce NE-PER final insoluble pellet, is it chromatin and how to solubilize it? - (reply: 1)
51. Predicting amount maximum of sample to apply onto cation exchange chromatography - (reply: 5)
52. recombinant protein vs native protein - (reply: 1)
53. protein expression site - (reply: 3)
54. Beads pull down only free GST - (reply: 14)
55. Where to obtain strain E. coli K12 SB536 ? - (reply: 3)
56. Protein Expresion and Isolation from Lactobacillus - Insoluble protein - (reply: 1)
57. protein expression - (reply: 2)
58. Anion Exchange Buffers - (reply: 2)
59. Difficulty eluting His-tag Zn metalloprotease - (reply: 1)
60. about sds-page buffers - (reply: 1)

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