|
31. membrane proteins ultrafiltration - (reply: 2)
32. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
33. Interferon Alpha - (reply: 1)
34. Can you see GFP after lysis mammalian cells? - (reply: 5)
35. Very small peptide expression in E. coli - (reply: 2)
36. C-terminal GST fusion problem in mammalian cell - (reply: 6)
37. Is anti-His antibody detergent sensitive? - (reply: 6)
38. tagged protein size - (reply: 10)
39. Protein expression in e.coli - (reply: 5)
40. Problems with overnights - (reply: 2)
41. problem in extracting the protein - (reply: 1)
42. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
43. Protein Purification from Polyacrylamide Gels - (reply: 3)
44. Protein Expression vector with small tag - (reply: 1)
45. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
46. Albumin Removal From BSA - (reply: 1)
47. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
48. Insulin production - (reply: 2)
49. High Yield Protein Expression 10g/L - (reply: 5)
50. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
51. GST- tagged protein expressed in insoluble - (reply: 5)
52. Scaling up reagents for large scale immunoprecipitation - (reply: 1)
53. Prepare protein standards for concentration determination - (reply: 1)
54. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)
55. Tagged recombinant protein has different mobility? - (reply: 4)
56. My protein doesn't bind to the Ni-NTA column - (reply: 4)
57. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 28)
58. TCA IgG precipitation - (reply: 2)
59. Does triple labeled protein precipitate more easily? - (reply: 2)
60. High gravity crush beads ? - (reply: 1)
32. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
33. Interferon Alpha - (reply: 1)
34. Can you see GFP after lysis mammalian cells? - (reply: 5)
35. Very small peptide expression in E. coli - (reply: 2)
36. C-terminal GST fusion problem in mammalian cell - (reply: 6)
37. Is anti-His antibody detergent sensitive? - (reply: 6)
38. tagged protein size - (reply: 10)
39. Protein expression in e.coli - (reply: 5)
40. Problems with overnights - (reply: 2)
41. problem in extracting the protein - (reply: 1)
42. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
43. Protein Purification from Polyacrylamide Gels - (reply: 3)
44. Protein Expression vector with small tag - (reply: 1)
45. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
46. Albumin Removal From BSA - (reply: 1)
47. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
48. Insulin production - (reply: 2)
49. High Yield Protein Expression 10g/L - (reply: 5)
50. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
51. GST- tagged protein expressed in insoluble - (reply: 5)
52. Scaling up reagents for large scale immunoprecipitation - (reply: 1)
53. Prepare protein standards for concentration determination - (reply: 1)
54. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)
55. Tagged recombinant protein has different mobility? - (reply: 4)
56. My protein doesn't bind to the Ni-NTA column - (reply: 4)
57. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 28)
58. TCA IgG precipitation - (reply: 2)
59. Does triple labeled protein precipitate more easily? - (reply: 2)
60. High gravity crush beads ? - (reply: 1)