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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
31. "Leaky" stepwise elution of active protein from Ion-Exchange columns - (reply: 2)
32. troubleshooting is needed for heme protein Ni-NTA purification - (reply: 2)
33. after gelfiltration need to concentrate peptide mix - (reply: 4)
34. Quick and simple protocol for protein purification and extraction, thoughts? - (reply: 2)
35. Protein that used to express really well stopped expressing. - (reply: 2)
36. Determining Cofactor for Inactive Enzyme - (reply: 6)
37. study protein expression in bacteria - (reply: 1)
38. total protein measurement from insect body - (reply: 1)
39. Does over-expression protein under T7 promoter in Top10 work? - (reply: 1)
40. Large-scale cell growth and protein expression - (reply: 1)
41. Reduce protein concentration without reduced enzyme activity - (reply: 3)
42. consistent expression of overlarge proteins - (reply: 1)
43. Cytoplasmic/nuclear fractionation with NP-40 then RIPA buffer combination? - (reply: 2)
44. Is AS pp + HIC phisically possible for all proteins? - (reply: 2)
45. storage conditions for purified protein - (reply: 2)
46. Need to include protease inhibitor in IP wash buffer? - (reply: 1)
47. Heat shock vs eletroporation - (reply: 6)
48. lysate protein concentration - (reply: 1)
49. Cloned gene in pET system not being expressed - (reply: 10)
50. recombinant protein expression- surface or internal? - (reply: 3)
51. His-tag protein not binding to resin - (reply: 9)
52. Protein localisation - IHC - (reply: 1)
53. Collagen Tipe I filtration - (reply: 1)
54. Removal of cleaved peptide after Protease cleavage - (reply: 4)
55. Bradford positive but no Protein on SDS PAGE - (reply: 4)
56. Disadvantage of crossflow filtration - (reply: 3)
57. Help with Superdex200 increase Column - (reply: 1)
58. Thrombin cleaved GST-fusion protein- multiple bands - (reply: 2)
59. Loss of protein expression by transformed bacteria - (reply: 4)
60. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)

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