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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
331. labeling a protein - (reply: 3)
332. strep-tag II purification not pure - how to make it pure with one step purification or rather do two step (reply: 3)
333. How to be good at prep? - Calling all protein experts (reply: 2)
334. Protein Migration Problem [S.O.S.] - Moves in SDS-PAGE but not in Native (reply: 8)
335. His-tagged protein purification - (reply: 3)
336. protein purification - (reply: 3)
337. pGEX vectors and GST expression - (reply: 3)
338. contamination in protein expression - I got a couple of questions (reply: 3)
339. Always expressing truncated protein - (reply: 3)
340. Over Experssion of A Protein - (reply: 3)
341. IPTG induction not observable or noticeable - (reply: 2)
342. Problem with protein purification By FPLC - (reply: 4)
343. Protein precipitation with TCA? - Protocols, suggestions, ideas, experiences (reply: 2)
344. His-tag proteins not eluted from Ni-Beads - (reply: 2)
345. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
346. Recombinant Antibody Production -Reducing / Non-reducing SDS page conditions - (reply: 2)
347. trouble with protein expression of high AT rich gene - (reply: 1)
348. Preparation of Purified Inclusion Bodies - (reply: 2)
349. Denaturing IB in 8 M Urea - (reply: 3)
350. Promega IVT Problems - Low expression (reply: 1)
351. weak bands - western blots (reply: 1)
352. problem in M9 media - m9 media (reply: 4)
353. Help for a purificaltion newbie - (reply: 2)
354. Immunioprecipitation from Jurkat cells?? - (reply: 1)
355. Problems with Soluble and Insoluble Protein Fraction - (reply: 4)
356. antibody HRP - (reply: 2)
357. Western blot transfer weak - (reply: 1)
358. Home-made Size-exclusion Columns? - (reply: 1)
359. how to get pure protein - (reply: 1)
360. co-Immunoprecipitation control question - co-IP (reply: 1)

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