Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
331. Protein stability at room temperature - (reply: 3)
332. Testing Solubility in different pH's and salt concentrations for NMR. - (reply: 2)
333. Help with protein expression - how to optimize expression of large GST fusion protein (reply: 4)
334. Protein purification from HEK 293 cells - (reply: 1)
335. Upscaling protein expression in E.coli - (reply: 2)
336. how to purify Fc fragment - Fc fragment purification after papain digestion (reply: 8)
337. nuclease contamination in protein prep - (reply: 4)
338. Problems Expressing from Kluyveromyces lactis - (reply: 1)
339. Pierce BCA Assay problem - Protein values bizarre (reply: 3)
340. Unable to Purify protein! - Protein purification (reply: 2)
341. Problem with Pierce GST spin purification column - (reply: 6)
342. HIS-tagged protein purification - (reply: 12)
343. how to elute "only" my recombinant protein - final elutes comes with non-specific proteins... (reply: 4)
344. His-tagged protein purification problem - (reply: 2)
345. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
346. Signal Peptides - To leave or not to leave (reply: 2)
347. Low expression of pET21a construct - (reply: 6)
348. GST-fusion protein degrades in-vivo - (reply: 3)
349. Inclusion body purification - (reply: 1)
350. help with protein elution - (reply: 1)
351. where are they on SDS-PAGE gel of Co-IPed complex??? - (reply: 1)
352. Active protein extract from corn - (reply: 2)
353. How to prepare high quality brain tissue lysate? - Will high salt lysis buffer be helpful? (reply: 1)
354. Reduced Glutathione affects thrombin activity? - (reply: 2)
355. You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)
356. Should I worry about endotoxin? - (reply: 4)
357. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
358. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
359. Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
360. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22