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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
331. Reporter gene to test new promotor - (reply: 1)
332. Protein Phosphorylation - (reply: 2)
333. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
334. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
335. Folding - (reply: 2)
336. C-terminal Cleavage During Overexpression - (reply: 3)
337. FOR immunoprecipitation in plant , flag tag or His tag - (reply: 2)
338. Loss of protein upon concentration - (reply: 4)
339. Problems with Bacterial Induction: NO Induction - (reply: 1)
340. His-tag protein purification - aggregation of protein (reply: 5)
341. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
342. Coimmunoprecipitation using Ni-NTA, My non-his-tagged target bind to beads - (reply: 3)
343. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
344. Decrease in concentration after protein purification - (reply: 3)
345. Ammonium sulphate precipitation Problem - Precipitants couldnt be seen on SDS-PAGE and WB (reply: 9)
346. Bacterial Induction: Same concentration of protein samples? - (reply: 1)
347. ways to stop soluble aggregation - (reply: 9)
348. need protocol on purfication of allinase from raw garlic - (reply: 2)
349. labeling a protein - (reply: 3)
350. strep-tag II purification not pure - how to make it pure with one step purification or rather do two step (reply: 3)
351. How to be good at prep? - Calling all protein experts (reply: 2)
352. Protein Migration Problem [S.O.S.] - Moves in SDS-PAGE but not in Native (reply: 8)
353. His-tagged protein purification - (reply: 3)
354. protein purification - (reply: 3)
355. pGEX vectors and GST expression - (reply: 3)
356. contamination in protein expression - I got a couple of questions (reply: 3)
357. Always expressing truncated protein - (reply: 3)
358. Over Experssion of A Protein - (reply: 3)
359. IPTG induction not observable or noticeable - (reply: 2)
360. Problem with protein purification By FPLC - (reply: 4)

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