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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
331. Testing Solubility in different pH's and salt concentrations for NMR. - (reply: 2)
332. Help with protein expression - how to optimize expression of large GST fusion protein (reply: 4)
333. Protein purification from HEK 293 cells - (reply: 1)
334. Upscaling protein expression in E.coli - (reply: 2)
335. how to purify Fc fragment - Fc fragment purification after papain digestion (reply: 8)
336. nuclease contamination in protein prep - (reply: 4)
337. Problems Expressing from Kluyveromyces lactis - (reply: 1)
338. Pierce BCA Assay problem - Protein values bizarre (reply: 3)
339. Unable to Purify protein! - Protein purification (reply: 2)
340. Problem with Pierce GST spin purification column - (reply: 6)
341. HIS-tagged protein purification - (reply: 12)
342. how to elute "only" my recombinant protein - final elutes comes with non-specific proteins... (reply: 4)
343. His-tagged protein purification problem - (reply: 2)
344. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
345. Signal Peptides - To leave or not to leave (reply: 2)
346. Low expression of pET21a construct - (reply: 6)
347. GST-fusion protein degrades in-vivo - (reply: 3)
348. Inclusion body purification - (reply: 1)
349. help with protein elution - (reply: 1)
350. where are they on SDS-PAGE gel of Co-IPed complex??? - (reply: 1)
351. Active protein extract from corn - (reply: 2)
352. How to prepare high quality brain tissue lysate? - Will high salt lysis buffer be helpful? (reply: 1)
353. Reduced Glutathione affects thrombin activity? - (reply: 2)
354. You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)
355. Should I worry about endotoxin? - (reply: 4)
356. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
357. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
358. Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
359. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
360. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)

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