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331. Dialysis - A question about buffers (reply: 6)
332. Purification problems his-tag - I have tried everything (reply: 3)
333. GST-tagged protein binds to column but is not eluted - (reply: 1)
334. urea-denatured protein precipitates after dialysis - (reply: 1)
335. Expressing 4 proteins in an operon - (reply: 6)
336. More His-tag problems - (reply: 4)
337. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
338. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
339. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
340. Protein precipitates upon dialysis - (reply: 5)
341. membrane protein aggregation - (reply: 1)
342. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
343. His Tag Protein Purification - (reply: 6)
344. Amylose bound protein elution - (reply: 1)
345. hey guys，what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
346. Soluble aggregates - (reply: 3)
347. insect protein expression - (reply: 1)
348. Can you store TNT translation products? - (reply: 1)
349. How to pack the FPLC column - (reply: 2)
350. Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
351. Sonication issues! - (reply: 3)
352. complete gene for expression? - (reply: 2)
353. buffer for membrane protein - (reply: 1)
354. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
355. Question: Bradford assay - buffer precipitates - KCl and HEPES preciptation with Bradford reagent (reply: 3)
356. membrane protein purification - (reply: 6)
357. Protein expression failing - (reply: 1)
358. How does aggregation influence absorbtion at 280nm? - (reply: 1)
359. Buttery Popcorn Odor in Contaminated Expression Prep - (reply: 3)
360. Self-made Protease inhibitor cocktail - PMSF, EDTA, Benzamidine (reply: 4)
332. Purification problems his-tag - I have tried everything (reply: 3)
333. GST-tagged protein binds to column but is not eluted - (reply: 1)
334. urea-denatured protein precipitates after dialysis - (reply: 1)
335. Expressing 4 proteins in an operon - (reply: 6)
336. More His-tag problems - (reply: 4)
337. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
338. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
339. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
340. Protein precipitates upon dialysis - (reply: 5)
341. membrane protein aggregation - (reply: 1)
342. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
343. His Tag Protein Purification - (reply: 6)
344. Amylose bound protein elution - (reply: 1)
345. hey guys，what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
346. Soluble aggregates - (reply: 3)
347. insect protein expression - (reply: 1)
348. Can you store TNT translation products? - (reply: 1)
349. How to pack the FPLC column - (reply: 2)
350. Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
351. Sonication issues! - (reply: 3)
352. complete gene for expression? - (reply: 2)
353. buffer for membrane protein - (reply: 1)
354. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
355. Question: Bradford assay - buffer precipitates - KCl and HEPES preciptation with Bradford reagent (reply: 3)
356. membrane protein purification - (reply: 6)
357. Protein expression failing - (reply: 1)
358. How does aggregation influence absorbtion at 280nm? - (reply: 1)
359. Buttery Popcorn Odor in Contaminated Expression Prep - (reply: 3)
360. Self-made Protease inhibitor cocktail - PMSF, EDTA, Benzamidine (reply: 4)