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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
331. IPTG induction not observable or noticeable - (reply: 2)
332. Problem with protein purification By FPLC - (reply: 4)
333. Protein precipitation with TCA? - Protocols, suggestions, ideas, experiences (reply: 2)
334. His-tag proteins not eluted from Ni-Beads - (reply: 2)
335. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
336. Recombinant Antibody Production -Reducing / Non-reducing SDS page conditions - (reply: 2)
337. trouble with protein expression of high AT rich gene - (reply: 1)
338. Preparation of Purified Inclusion Bodies - (reply: 2)
339. Denaturing IB in 8 M Urea - (reply: 3)
340. Promega IVT Problems - Low expression (reply: 1)
341. weak bands - western blots (reply: 1)
342. problem in M9 media - m9 media (reply: 4)
343. Help for a purificaltion newbie - (reply: 2)
344. Immunioprecipitation from Jurkat cells?? - (reply: 1)
345. Problems with Soluble and Insoluble Protein Fraction - (reply: 4)
346. antibody HRP - (reply: 2)
347. Western blot transfer weak - (reply: 1)
348. Home-made Size-exclusion Columns? - (reply: 1)
349. how to get pure protein - (reply: 1)
350. co-Immunoprecipitation control question - co-IP (reply: 1)
351. to get protein in cytosol from inclusion bodies - (reply: 4)
352. Inclusion bodies in e.coli - (reply: 1)
353. lysis of e.coli by sonication - (reply: 2)
354. Looking for precipitation method to reduce the volume of my protein complex elu - TCA precipitation seems to be hard to be resolved again (reply: 6)
355. Protein expression - (reply: 8)
356. problem with ion exchange puification - protein not binding (reply: 3)
357. Protein Purification by precipitation - (reply: 2)
358. refolding proteins using dialysis - (reply: 1)
359. protein refolding - (reply: 1)
360. Zeocine resistence, pichia pastoris - (reply: 3)

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