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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
331. protein solution cloudy after dialysis - (reply: 5)
332. Butyl-agarose/sepharose purification - (reply: 1)
333. Low or even None expression - (reply: 1)
334. How can I detect mannosidase activity of a protein - (reply: 1)
335. Protein Aggregation with 6xHis tagged CreGFP - (reply: 1)
336. Protein stability at room temperature - (reply: 3)
337. Testing Solubility in different pH's and salt concentrations for NMR. - (reply: 2)
338. Help with protein expression - how to optimize expression of large GST fusion protein (reply: 4)
339. Protein purification from HEK 293 cells - (reply: 1)
340. Upscaling protein expression in E.coli - (reply: 2)
341. how to purify Fc fragment - Fc fragment purification after papain digestion (reply: 8)
342. nuclease contamination in protein prep - (reply: 4)
343. Problems Expressing from Kluyveromyces lactis - (reply: 1)
344. Pierce BCA Assay problem - Protein values bizarre (reply: 3)
345. Unable to Purify protein! - Protein purification (reply: 2)
346. Problem with Pierce GST spin purification column - (reply: 6)
347. HIS-tagged protein purification - (reply: 12)
348. how to elute "only" my recombinant protein - final elutes comes with non-specific proteins... (reply: 4)
349. His-tagged protein purification problem - (reply: 2)
350. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
351. Signal Peptides - To leave or not to leave (reply: 2)
352. Low expression of pET21a construct - (reply: 6)
353. GST-fusion protein degrades in-vivo - (reply: 3)
354. Inclusion body purification - (reply: 1)
355. help with protein elution - (reply: 1)
356. where are they on SDS-PAGE gel of Co-IPed complex??? - (reply: 1)
357. Active protein extract from corn - (reply: 2)
358. How to prepare high quality brain tissue lysate? - Will high salt lysis buffer be helpful? (reply: 1)
359. Reduced Glutathione affects thrombin activity? - (reply: 2)
360. You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)

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