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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
331. His-tagged protein purification problem - (reply: 2)
332. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
333. Signal Peptides - To leave or not to leave (reply: 2)
334. Low expression of pET21a construct - (reply: 6)
335. GST-fusion protein degrades in-vivo - (reply: 3)
336. Inclusion body purification - (reply: 1)
337. help with protein elution - (reply: 1)
338. where are they on SDS-PAGE gel of Co-IPed complex??? - (reply: 1)
339. Active protein extract from corn - (reply: 2)
340. How to prepare high quality brain tissue lysate? - Will high salt lysis buffer be helpful? (reply: 1)
341. Reduced Glutathione affects thrombin activity? - (reply: 2)
342. You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)
343. Should I worry about endotoxin? - (reply: 4)
344. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
345. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
346. Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
347. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
348. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
349. Western Blot of Large Protein Help - (reply: 1)
350. how to make protein soluble? - protein solubility (reply: 4)
351. protein yield is very low after purification - protein purification (reply: 9)
352. FZR1 PROTEIN EXPRESSION - (reply: 1)
353. Membrane solubilisation - (reply: 2)
354. Heterologous protein extraction in yeast for enzymatic activity test - (reply: 5)
355. His tag protein purification - (reply: 7)
356. Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
357. Protein precipitating need help choosing suitable buffering system - (reply: 3)
358. Expressing human protein in E.Coli - (reply: 12)
359. Sample preparation for SDS from detergent insoluble fraction (Pellet). - (reply: 2)
360. Help with GST protein purification - (reply: 4)

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