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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
331. Problems Expressing from Kluyveromyces lactis - (reply: 1)
332. Pierce BCA Assay problem - Protein values bizarre (reply: 3)
333. Unable to Purify protein! - Protein purification (reply: 2)
334. Problem with Pierce GST spin purification column - (reply: 6)
335. HIS-tagged protein purification - (reply: 12)
336. how to elute "only" my recombinant protein - final elutes comes with non-specific proteins... (reply: 4)
337. His-tagged protein purification problem - (reply: 2)
338. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
339. Signal Peptides - To leave or not to leave (reply: 2)
340. Low expression of pET21a construct - (reply: 6)
341. GST-fusion protein degrades in-vivo - (reply: 3)
342. Inclusion body purification - (reply: 1)
343. help with protein elution - (reply: 1)
344. where are they on SDS-PAGE gel of Co-IPed complex??? - (reply: 1)
345. Active protein extract from corn - (reply: 2)
346. How to prepare high quality brain tissue lysate? - Will high salt lysis buffer be helpful? (reply: 1)
347. Reduced Glutathione affects thrombin activity? - (reply: 2)
348. You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)
349. Should I worry about endotoxin? - (reply: 4)
350. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
351. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
352. Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
353. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
354. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
355. Western Blot of Large Protein Help - (reply: 1)
356. how to make protein soluble? - protein solubility (reply: 4)
357. protein yield is very low after purification - protein purification (reply: 9)
358. FZR1 PROTEIN EXPRESSION - (reply: 1)
359. Membrane solubilisation - (reply: 2)
360. Heterologous protein extraction in yeast for enzymatic activity test - (reply: 5)

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