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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
331. His tag protein purification - (reply: 7)
332. Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
333. Protein precipitating need help choosing suitable buffering system - (reply: 3)
334. Expressing human protein in E.Coli - (reply: 12)
335. Sample preparation for SDS from detergent insoluble fraction (Pellet). - (reply: 2)
336. Help with GST protein purification - (reply: 4)
337. Antiobiotics for protein expression - (reply: 3)
338. how to extract proteins from HEK293 - I need your experience (reply: 3)
339. Identification of purity of the protein - (reply: 2)
340. Polysorbate 80 (Tween 80) removal - (reply: 13)
341. Maltose Binding Protein Sequence - A sequence for the Maltose Binding Protein. (reply: 1)
342. nuclear extraction of blood cells - (reply: 2)
343. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
344. volume to load sample for affinity chromatography - (reply: 6)
345. Reporter gene to test new promotor - (reply: 1)
346. Protein Phosphorylation - (reply: 2)
347. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
348. His-tag purification post Enterokinase digestion - Buffer exchange? (reply: 1)
349. Folding - (reply: 2)
350. C-terminal Cleavage During Overexpression - (reply: 3)
351. FOR immunoprecipitation in plant , flag tag or His tag - (reply: 2)
352. Loss of protein upon concentration - (reply: 4)
353. Problems with Bacterial Induction: NO Induction - (reply: 1)
354. His-tag protein purification - aggregation of protein (reply: 5)
355. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
356. Coimmunoprecipitation using Ni-NTA, My non-his-tagged target bind to beads - (reply: 3)
357. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
358. Decrease in concentration after protein purification - (reply: 3)
359. Ammonium sulphate precipitation Problem - Precipitants couldnt be seen on SDS-PAGE and WB (reply: 9)
360. Bacterial Induction: Same concentration of protein samples? - (reply: 1)

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