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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
331. Problem with Pierce GST spin purification column - (reply: 6)
332. HIS-tagged protein purification - (reply: 12)
333. how to elute "only" my recombinant protein - final elutes comes with non-specific proteins... (reply: 4)
334. His-tagged protein purification problem - (reply: 2)
335. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
336. Signal Peptides - To leave or not to leave (reply: 2)
337. Low expression of pET21a construct - (reply: 6)
338. GST-fusion protein degrades in-vivo - (reply: 3)
339. Inclusion body purification - (reply: 1)
340. help with protein elution - (reply: 1)
341. where are they on SDS-PAGE gel of Co-IPed complex??? - (reply: 1)
342. Active protein extract from corn - (reply: 2)
343. How to prepare high quality brain tissue lysate? - Will high salt lysis buffer be helpful? (reply: 1)
344. Reduced Glutathione affects thrombin activity? - (reply: 2)
345. You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)
346. Should I worry about endotoxin? - (reply: 4)
347. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
348. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
349. Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
350. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
351. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
352. Western Blot of Large Protein Help - (reply: 1)
353. how to make protein soluble? - protein solubility (reply: 4)
354. protein yield is very low after purification - protein purification (reply: 9)
355. FZR1 PROTEIN EXPRESSION - (reply: 1)
356. Membrane solubilisation - (reply: 2)
357. Heterologous protein extraction in yeast for enzymatic activity test - (reply: 5)
358. His tag protein purification - (reply: 7)
359. Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
360. Protein precipitating need help choosing suitable buffering system - (reply: 3)

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