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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
331. pGEX vectors and GST expression - (reply: 3)
332. contamination in protein expression - I got a couple of questions (reply: 3)
333. Always expressing truncated protein - (reply: 3)
334. Over Experssion of A Protein - (reply: 3)
335. IPTG induction not observable or noticeable - (reply: 2)
336. Problem with protein purification By FPLC - (reply: 4)
337. Protein precipitation with TCA? - Protocols, suggestions, ideas, experiences (reply: 2)
338. His-tag proteins not eluted from Ni-Beads - (reply: 2)
339. Expressing a Gene or an ORF in an Expression Vector? - (reply: 4)
340. Recombinant Antibody Production -Reducing / Non-reducing SDS page conditions - (reply: 2)
341. trouble with protein expression of high AT rich gene - (reply: 1)
342. Preparation of Purified Inclusion Bodies - (reply: 2)
343. Denaturing IB in 8 M Urea - (reply: 3)
344. Promega IVT Problems - Low expression (reply: 1)
345. weak bands - western blots (reply: 1)
346. problem in M9 media - m9 media (reply: 4)
347. Help for a purificaltion newbie - (reply: 2)
348. Immunioprecipitation from Jurkat cells?? - (reply: 1)
349. Problems with Soluble and Insoluble Protein Fraction - (reply: 4)
350. antibody HRP - (reply: 2)
351. Western blot transfer weak - (reply: 1)
352. Home-made Size-exclusion Columns? - (reply: 1)
353. how to get pure protein - (reply: 1)
354. co-Immunoprecipitation control question - co-IP (reply: 1)
355. to get protein in cytosol from inclusion bodies - (reply: 4)
356. Inclusion bodies in e.coli - (reply: 1)
357. lysis of e.coli by sonication - (reply: 2)
358. Looking for precipitation method to reduce the volume of my protein complex elu - TCA precipitation seems to be hard to be resolved again (reply: 6)
359. Protein expression - (reply: 8)
360. problem with ion exchange puification - protein not binding (reply: 3)

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