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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
331. Upscaling protein expression in E.coli - (reply: 2)
332. how to purify Fc fragment - Fc fragment purification after papain digestion (reply: 8)
333. nuclease contamination in protein prep - (reply: 4)
334. Problems Expressing from Kluyveromyces lactis - (reply: 1)
335. Pierce BCA Assay problem - Protein values bizarre (reply: 3)
336. Unable to Purify protein! - Protein purification (reply: 2)
337. Problem with Pierce GST spin purification column - (reply: 6)
338. HIS-tagged protein purification - (reply: 12)
339. how to elute "only" my recombinant protein - final elutes comes with non-specific proteins... (reply: 4)
340. His-tagged protein purification problem - (reply: 2)
341. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
342. Signal Peptides - To leave or not to leave (reply: 2)
343. Low expression of pET21a construct - (reply: 6)
344. GST-fusion protein degrades in-vivo - (reply: 3)
345. Inclusion body purification - (reply: 1)
346. help with protein elution - (reply: 1)
347. where are they on SDS-PAGE gel of Co-IPed complex??? - (reply: 1)
348. Active protein extract from corn - (reply: 2)
349. How to prepare high quality brain tissue lysate? - Will high salt lysis buffer be helpful? (reply: 1)
350. Reduced Glutathione affects thrombin activity? - (reply: 2)
351. You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)
352. Should I worry about endotoxin? - (reply: 4)
353. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
354. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
355. Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
356. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
357. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
358. Western Blot of Large Protein Help - (reply: 1)
359. how to make protein soluble? - protein solubility (reply: 4)
360. protein yield is very low after purification - protein purification (reply: 9)

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