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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
331. HIS-tagged protein purification - (reply: 12)
332. how to elute "only" my recombinant protein - final elutes comes with non-specific proteins... (reply: 4)
333. His-tagged protein purification problem - (reply: 2)
334. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
335. Signal Peptides - To leave or not to leave (reply: 2)
336. Low expression of pET21a construct - (reply: 6)
337. GST-fusion protein degrades in-vivo - (reply: 3)
338. Inclusion body purification - (reply: 1)
339. help with protein elution - (reply: 1)
340. where are they on SDS-PAGE gel of Co-IPed complex??? - (reply: 1)
341. Active protein extract from corn - (reply: 2)
342. How to prepare high quality brain tissue lysate? - Will high salt lysis buffer be helpful? (reply: 1)
343. Reduced Glutathione affects thrombin activity? - (reply: 2)
344. You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)
345. Should I worry about endotoxin? - (reply: 4)
346. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
347. Avoidance of leaky expression in Terrific Broth - did I need to add 1% Glucose (reply: 5)
348. Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
349. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
350. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
351. Western Blot of Large Protein Help - (reply: 1)
352. how to make protein soluble? - protein solubility (reply: 4)
353. protein yield is very low after purification - protein purification (reply: 9)
354. FZR1 PROTEIN EXPRESSION - (reply: 1)
355. Membrane solubilisation - (reply: 2)
356. Heterologous protein extraction in yeast for enzymatic activity test - (reply: 5)
357. His tag protein purification - (reply: 7)
358. Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
359. Protein precipitating need help choosing suitable buffering system - (reply: 3)
360. Expressing human protein in E.Coli - (reply: 12)

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