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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
121. selection of the media for ion exchange chromatography - (reply: 1)
122. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
123. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
124. Does a C-terminal Proline Residue Increase Degradation Risk? - (reply: 2)
125. His-tag and GST-tag - (reply: 1)
126. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
127. Do protein purification under partially denatured condition - (reply: 1)
128. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
129. how to remove affinity tag completely - (reply: 14)
130. Agarose bead size in affinity chromatography - (reply: 2)
131. Protein precipitation by storage - (reply: 11)
132. designing fusion protein with Flag tag - (reply: 1)
133. Problems with biotynylated protein binding to monomeric avidin - (reply: 1)
134. Control protein for eukaryotic expression - (reply: 5)
135. Flag fusion protein not detected by Flag antibody - (reply: 8)
136. E. coli culture - (reply: 1)
137. instability index of expressed protein - (reply: 1)
138. Protein Purification-catastrophe while purifying? - (reply: 2)
139. T5 Promoter - (reply: 1)
140. Protein purification for adherent mammalian cells - (reply: 2)
141. Antibody production in Expi293 cells - (reply: 2)
142. pGEX-2t purification problem - (reply: 3)
143. Phosphorylation of recombinant proteins in E. coli - (reply: 1)
144. Compatibility of Urea and Imidazole - (reply: 2)
145. Growth media - OD at induction - growth phases... - (reply: 2)
146. NusA tag produces protein truncations? - (reply: 3)
147. Tissue-Specific Protein Expression Database - (reply: 4)
148. Problem with strep tagged protein purification - (reply: 5)
149. why i get double band for recombinant protein - (reply: 9)
150. Pulldown with nickel beads? - (reply: 3)

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