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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
121. protein extraction from cell nucleus - (reply: 2)
122. his-tag purification - (reply: 9)
123. selection of the media for ion exchange chromatography - (reply: 1)
124. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
125. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
126. Does a C-terminal Proline Residue Increase Degradation Risk? - (reply: 2)
127. His-tag and GST-tag - (reply: 1)
128. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
129. Do protein purification under partially denatured condition - (reply: 1)
130. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
131. how to remove affinity tag completely - (reply: 14)
132. Agarose bead size in affinity chromatography - (reply: 2)
133. Protein precipitation by storage - (reply: 11)
134. designing fusion protein with Flag tag - (reply: 1)
135. Problems with biotynylated protein binding to monomeric avidin - (reply: 1)
136. Control protein for eukaryotic expression - (reply: 5)
137. Flag fusion protein not detected by Flag antibody - (reply: 8)
138. E. coli culture - (reply: 1)
139. instability index of expressed protein - (reply: 1)
140. Protein Purification-catastrophe while purifying? - (reply: 2)
141. T5 Promoter - (reply: 1)
142. Protein purification for adherent mammalian cells - (reply: 2)
143. Antibody production in Expi293 cells - (reply: 2)
144. pGEX-2t purification problem - (reply: 3)
145. Phosphorylation of recombinant proteins in E. coli - (reply: 1)
146. Compatibility of Urea and Imidazole - (reply: 2)
147. Growth media - OD at induction - growth phases... - (reply: 2)
148. NusA tag produces protein truncations? - (reply: 3)
149. Tissue-Specific Protein Expression Database - (reply: 4)
150. Problem with strep tagged protein purification - (reply: 5)

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