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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
121. his-tag purification - (reply: 9)
122. selection of the media for ion exchange chromatography - (reply: 1)
123. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
124. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
125. Does a C-terminal Proline Residue Increase Degradation Risk? - (reply: 2)
126. His-tag and GST-tag - (reply: 1)
127. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
128. Do protein purification under partially denatured condition - (reply: 1)
129. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
130. how to remove affinity tag completely - (reply: 14)
131. Agarose bead size in affinity chromatography - (reply: 2)
132. Protein precipitation by storage - (reply: 11)
133. designing fusion protein with Flag tag - (reply: 1)
134. Problems with biotynylated protein binding to monomeric avidin - (reply: 1)
135. Control protein for eukaryotic expression - (reply: 5)
136. Flag fusion protein not detected by Flag antibody - (reply: 8)
137. E. coli culture - (reply: 1)
138. instability index of expressed protein - (reply: 1)
139. Protein Purification-catastrophe while purifying? - (reply: 2)
140. T5 Promoter - (reply: 1)
141. Protein purification for adherent mammalian cells - (reply: 2)
142. Antibody production in Expi293 cells - (reply: 2)
143. pGEX-2t purification problem - (reply: 3)
144. Phosphorylation of recombinant proteins in E. coli - (reply: 1)
145. Compatibility of Urea and Imidazole - (reply: 2)
146. Growth media - OD at induction - growth phases... - (reply: 2)
147. NusA tag produces protein truncations? - (reply: 3)
148. Tissue-Specific Protein Expression Database - (reply: 4)
149. Problem with strep tagged protein purification - (reply: 5)
150. why i get double band for recombinant protein - (reply: 9)

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