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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
121. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
122. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
123. Will 5mM DTT break existing disulfide bonds? - (reply: 3)
124. Just part of the CDS (like transmembrane domain) or the full-length CDS for prot - (reply: 3)
125. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
126. protein extraction from cell nucleus - (reply: 2)
127. his-tag purification - (reply: 9)
128. selection of the media for ion exchange chromatography - (reply: 1)
129. Designing rPlasmid for Expression in E.Coli: Questions From A Newbie - (reply: 1)
130. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
131. Does a C-terminal Proline Residue Increase Degradation Risk? - (reply: 2)
132. His-tag and GST-tag - (reply: 1)
133. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
134. Do protein purification under partially denatured condition - (reply: 1)
135. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
136. how to remove affinity tag completely - (reply: 14)
137. Agarose bead size in affinity chromatography - (reply: 2)
138. Protein precipitation by storage - (reply: 11)
139. designing fusion protein with Flag tag - (reply: 1)
140. Problems with biotynylated protein binding to monomeric avidin - (reply: 1)
141. Control protein for eukaryotic expression - (reply: 5)
142. Flag fusion protein not detected by Flag antibody - (reply: 8)
143. E. coli culture - (reply: 1)
144. instability index of expressed protein - (reply: 1)
145. Protein Purification-catastrophe while purifying? - (reply: 2)
146. T5 Promoter - (reply: 1)
147. Protein purification for adherent mammalian cells - (reply: 2)
148. Antibody production in Expi293 cells - (reply: 2)
149. pGEX-2t purification problem - (reply: 3)
150. Phosphorylation of recombinant proteins in E. coli - (reply: 1)

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