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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
121. Protein purifcation without chromatography - (reply: 6)
122. Bacterial Media Formulation - (reply: 2)
123. tag for protein purification - (reply: 7)
124. Protein is not Binding to DE52 column material - (reply: 2)
125. expression problem with pBAD33...similar experience? - (reply: 2)
126. MBP-Tagged protein degrading after expression - (reply: 1)
127. How to prevent albumin masking of urokinase from conditioned media - (reply: 3)
128. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
129. Controls for co-immunoprecipitation - (reply: 3)
130. How to improve enzyme activity? - (reply: 2)
131. Protein puriification using IMAC: flowthrough very slow - (reply: 2)
132. Sediments/debris/pellet in protein lysate? - (reply: 3)
133. Cleavage of GST-tag with Factor Xa - (reply: 1)
134. Trouble in GST- fusion protein purification - (reply: 1)
135. Helppurification of expressed protein - (reply: 2)
136. protein expression by changing start codon - (reply: 1)
137. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
138. Odd secondary pellet formed after lysis with B-Per or BugBuster - (reply: 2)
139. pcDNA overexpression - (reply: 4)
140. membrane proteins ultrafiltration - (reply: 2)
141. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
142. Interferon Alpha - (reply: 1)
143. Can you see GFP after lysis mammalian cells? - (reply: 5)
144. Very small peptide expression in E. coli - (reply: 2)
145. C-terminal GST fusion problem in mammalian cell - (reply: 6)
146. Is anti-His antibody detergent sensitive? - (reply: 6)
147. tagged protein size - (reply: 10)
148. Protein expression in e.coli - (reply: 5)
149. Problems with overnights - (reply: 5)
150. problem in extracting the protein - (reply: 1)

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