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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
121. MBP-Tagged protein degrading after expression - (reply: 1)
122. How to prevent albumin masking of urokinase from conditioned media - (reply: 3)
123. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
124. Controls for co-immunoprecipitation - (reply: 3)
125. How to improve enzyme activity? - (reply: 2)
126. Protein puriification using IMAC: flowthrough very slow - (reply: 2)
127. Sediments/debris/pellet in protein lysate? - (reply: 3)
128. Cleavage of GST-tag with Factor Xa - (reply: 1)
129. Trouble in GST- fusion protein purification - (reply: 1)
130. Helppurification of expressed protein - (reply: 2)
131. protein expression by changing start codon - (reply: 1)
132. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
133. Odd secondary pellet formed after lysis with B-Per or BugBuster - (reply: 2)
134. pcDNA overexpression - (reply: 4)
135. membrane proteins ultrafiltration - (reply: 2)
136. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
137. Interferon Alpha - (reply: 1)
138. Can you see GFP after lysis mammalian cells? - (reply: 5)
139. Very small peptide expression in E. coli - (reply: 2)
140. C-terminal GST fusion problem in mammalian cell - (reply: 6)
141. Is anti-His antibody detergent sensitive? - (reply: 6)
142. tagged protein size - (reply: 10)
143. Protein expression in e.coli - (reply: 5)
144. Problems with overnights - (reply: 5)
145. problem in extracting the protein - (reply: 1)
146. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
147. Protein Purification from Polyacrylamide Gels - (reply: 3)
148. Protein Expression vector with small tag - (reply: 1)
149. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
150. Albumin Removal From BSA - (reply: 1)

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