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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
121. pcDNA overexpression - (reply: 4)
122. membrane proteins ultrafiltration - (reply: 2)
123. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
124. Interferon Alpha - (reply: 1)
125. Can you see GFP after lysis mammalian cells? - (reply: 5)
126. Very small peptide expression in E. coli - (reply: 2)
127. C-terminal GST fusion problem in mammalian cell - (reply: 6)
128. Is anti-His antibody detergent sensitive? - (reply: 6)
129. tagged protein size - (reply: 10)
130. Protein expression in e.coli - (reply: 5)
131. Problems with overnights - (reply: 5)
132. problem in extracting the protein - (reply: 1)
133. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
134. Protein Purification from Polyacrylamide Gels - (reply: 3)
135. Protein Expression vector with small tag - (reply: 1)
136. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
137. Albumin Removal From BSA - (reply: 1)
138. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
139. Insulin production - (reply: 2)
140. High Yield Protein Expression 10g/L - (reply: 5)
141. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
142. GST- tagged protein expressed in insoluble - (reply: 5)
143. Scaling up reagents for large scale immunoprecipitation - (reply: 1)
144. Prepare protein standards for concentration determination - (reply: 1)
145. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)
146. Tagged recombinant protein has different mobility? - (reply: 4)
147. My protein doesn't bind to the Ni-NTA column - (reply: 11)
148. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
149. TCA IgG precipitation - (reply: 2)
150. Does triple labeled protein precipitate more easily? - (reply: 2)

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