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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
121. Soluble protien of GST - (reply: 2)
122. trouble in his-tagged protein binding with Ni-NTA agarose - (reply: 3)
123. Contaminants in protein purification.....help! - (reply: 3)
124. Protein expression and purification - Please help - (reply: 2)
125. Protein purifcation without chromatography - (reply: 6)
126. Bacterial Media Formulation - (reply: 2)
127. tag for protein purification - (reply: 7)
128. Protein is not Binding to DE52 column material - (reply: 2)
129. expression problem with pBAD33...similar experience? - (reply: 2)
130. MBP-Tagged protein degrading after expression - (reply: 1)
131. How to prevent albumin masking of urokinase from conditioned media - (reply: 3)
132. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
133. Controls for co-immunoprecipitation - (reply: 3)
134. How to improve enzyme activity? - (reply: 2)
135. Protein puriification using IMAC: flowthrough very slow - (reply: 2)
136. Sediments/debris/pellet in protein lysate? - (reply: 3)
137. Cleavage of GST-tag with Factor Xa - (reply: 1)
138. Trouble in GST- fusion protein purification - (reply: 1)
139. Helppurification of expressed protein - (reply: 2)
140. protein expression by changing start codon - (reply: 1)
141. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
142. Odd secondary pellet formed after lysis with B-Per or BugBuster - (reply: 2)
143. pcDNA overexpression - (reply: 4)
144. membrane proteins ultrafiltration - (reply: 2)
145. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
146. Interferon Alpha - (reply: 1)
147. Can you see GFP after lysis mammalian cells? - (reply: 5)
148. Very small peptide expression in E. coli - (reply: 2)
149. C-terminal GST fusion problem in mammalian cell - (reply: 6)
150. Is anti-His antibody detergent sensitive? - (reply: 6)

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