Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
121. Trouble in GST- fusion protein purification - (reply: 1)
122. Helppurification of expressed protein - (reply: 2)
123. protein expression by changing start codon - (reply: 1)
124. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
125. Odd secondary pellet formed after lysis with B-Per or BugBuster - (reply: 2)
126. pcDNA overexpression - (reply: 4)
127. membrane proteins ultrafiltration - (reply: 2)
128. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
129. Interferon Alpha - (reply: 1)
130. Can you see GFP after lysis mammalian cells? - (reply: 5)
131. Very small peptide expression in E. coli - (reply: 2)
132. C-terminal GST fusion problem in mammalian cell - (reply: 6)
133. Is anti-His antibody detergent sensitive? - (reply: 6)
134. tagged protein size - (reply: 10)
135. Protein expression in e.coli - (reply: 5)
136. Problems with overnights - (reply: 5)
137. problem in extracting the protein - (reply: 1)
138. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
139. Protein Purification from Polyacrylamide Gels - (reply: 3)
140. Protein Expression vector with small tag - (reply: 1)
141. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
142. Albumin Removal From BSA - (reply: 1)
143. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
144. Insulin production - (reply: 2)
145. High Yield Protein Expression 10g/L - (reply: 5)
146. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
147. GST- tagged protein expressed in insoluble - (reply: 5)
148. Scaling up reagents for large scale immunoprecipitation - (reply: 1)
149. Prepare protein standards for concentration determination - (reply: 1)
150. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20