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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
121. Lysis buffer for leptin receptor and pSTAT detection - (reply: 1)
122. Does a C-terminal Proline Residue Increase Degradation Risk? - (reply: 2)
123. His-tag and GST-tag - (reply: 1)
124. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
125. Do protein purification under partially denatured condition - (reply: 1)
126. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
127. how to remove affinity tag completely - (reply: 14)
128. Agarose bead size in affinity chromatography - (reply: 2)
129. Protein precipitation by storage - (reply: 11)
130. designing fusion protein with Flag tag - (reply: 1)
131. Problems with biotynylated protein binding to monomeric avidin - (reply: 1)
132. Control protein for eukaryotic expression - (reply: 5)
133. Flag fusion protein not detected by Flag antibody - (reply: 8)
134. E. coli culture - (reply: 1)
135. instability index of expressed protein - (reply: 1)
136. Protein Purification-catastrophe while purifying? - (reply: 2)
137. T5 Promoter - (reply: 1)
138. Protein purification for adherent mammalian cells - (reply: 2)
139. Antibody production in Expi293 cells - (reply: 2)
140. pGEX-2t purification problem - (reply: 3)
141. Phosphorylation of recombinant proteins in E. coli - (reply: 1)
142. Compatibility of Urea and Imidazole - (reply: 2)
143. Growth media - OD at induction - growth phases... - (reply: 2)
144. NusA tag produces protein truncations? - (reply: 3)
145. Tissue-Specific Protein Expression Database - (reply: 4)
146. Problem with strep tagged protein purification - (reply: 5)
147. why i get double band for recombinant protein - (reply: 9)
148. Pulldown with nickel beads? - (reply: 3)
149. protein purification in acanthamoeba lysate - (reply: 3)
150. GPCR expression in HEK293 - (reply: 2)

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