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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
121. Problems with overnights - (reply: 5)
122. problem in extracting the protein - (reply: 1)
123. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
124. Protein Purification from Polyacrylamide Gels - (reply: 3)
125. Protein Expression vector with small tag - (reply: 1)
126. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
127. Albumin Removal From BSA - (reply: 1)
128. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
129. Insulin production - (reply: 2)
130. High Yield Protein Expression 10g/L - (reply: 5)
131. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
132. GST- tagged protein expressed in insoluble - (reply: 5)
133. Scaling up reagents for large scale immunoprecipitation - (reply: 1)
134. Prepare protein standards for concentration determination - (reply: 1)
135. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)
136. Tagged recombinant protein has different mobility? - (reply: 4)
137. My protein doesn't bind to the Ni-NTA column - (reply: 11)
138. pET14b vector with insert transformed in BL21(DE3) RIPL cells - (reply: 30)
139. TCA IgG precipitation - (reply: 2)
140. Does triple labeled protein precipitate more easily? - (reply: 2)
141. High gravity crush beads ? - (reply: 1)
142. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
143. how do GST-tags affect gel filtration results? - (reply: 5)
144. protein extraction from human tissue (very small) to do ELISA - (reply: 5)
145. Stable protein expression in Vero cells - (reply: 2)
146. Which kind of ATP do you usually use in you experiment? - (reply: 2)
147. protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
148. GST-tagged protein expression problem! :-( - (reply: 4)
149. Better (quicker) way to pipet glycerol? - (reply: 3)
150. How OD600 influence on IPTG induction and protein expression - (reply: 5)

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