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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
481. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
482. What make voltage not going up for the 3rd step in running IEF? - (reply: 2)
483. Help...purify and concentrate protein for IEF - (reply: 1)
484. preference of N/C terminus for tagging - (reply: 2)
485. NI-NTA purification - (reply: 2)
486. Could a 30aa peptides(3×flag+6×his) be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)
487. Ampicillin Mess-up(Experiment in progress atm) - Experiment still valid? (reply: 1)
488. RNA immunoprecipitation...HELP! - (reply: 4)
489. Dialysis - A question about buffers (reply: 6)
490. Purification problems his-tag - I have tried everything (reply: 3)
491. GST-tagged protein binds to column but is not eluted - (reply: 1)
492. urea-denatured protein precipitates after dialysis - (reply: 1)
493. Expressing 4 proteins in an operon - (reply: 6)
494. More His-tag problems - (reply: 4)
495. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
496. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
497. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
498. Protein precipitates upon dialysis - (reply: 5)
499. membrane protein aggregation - (reply: 1)
500. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
501. His Tag Protein Purification - (reply: 6)
502. Amylose bound protein elution - (reply: 1)
503. hey guys,what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
504. Soluble aggregates - (reply: 3)
505. insect protein expression - (reply: 1)
506. Can you store TNT translation products? - (reply: 1)
507. How to pack the FPLC column - (reply: 2)
508. Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
509. Sonication issues! - (reply: 3)
510. complete gene for expression? - (reply: 2)

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