Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
481. protein expression (temperature issue) - trying to express soluble protein at lower temperature (reply: 17)
482. Can GST-tag be separated from GST-fusion protein - (reply: 1)
483. Protein analog - (reply: 1)
484. membrane protein extraction buffer - (reply: 1)
485. What about the Baculovirus expression system? - (reply: 2)
486. Ni-NTA beads and dialysis tubing - Purification using nickel beads (reply: 4)
487. Concentrating Protein with centrifuge concentrator but 50% glycerol!!? - (reply: 2)
488. protein is degraded after purification - (reply: 2)
489. protein expression - Preparation of starter cultures (reply: 15)
490. Problem with Protein elution using GST sepharose 4B - (reply: 1)
491. how to isolate insoluble protein in bacterial induction? - (reply: 4)
492. Plasmid instability in E.coli BL21? - (reply: 3)
493. Cleaving Fusion Proteins - (reply: 1)
494. Plyse cell and DH5a - (reply: 1)
495. Quality of aliquot for SDS-page in protein purification - When is pure pure enough? (reply: 1)
496. Electroelution with no electroeluter - (reply: 3)
497. reusing Ni NTA - (reply: 2)
498. Low expression: What else can I do? - Using pET23a in BL21 PlysS (reply: 9)
499. Changing dialysis buffer every half hour and then dialysis ON - protein precipitation prevention (reply: 1)
500. Checking For Protein Expression - (reply: 3)
501. How do I get rid of this contaminant? - His-tagged protein eluted with 300mM imidazole (reply: 13)
502. Is it possible? Harsh sonificiation conditions cause degradation of my protein - (reply: 3)
503. Low Thrombin Cleavage Efficiency Problem - I am trying to cleave a GST tagged protein(about 35 kDa + 26 kDa GST) (reply: 1)
504. Throbin cleavage declines with older protein? - It used to work, now doesn't! (reply: 1)
505. Couple questions about GST purification - (reply: 16)
506. Immunoprecipitation - (reply: 12)
507. Newbie question: how to determine protein concentration after IP? - (reply: 8)
508. protein extraction using trizol - can we replace guanidine cholride with other denaturants? (reply: 1)
509. High pressure on Ni-NTA beads - (reply: 2)
510. affinity column problems - (reply: 1)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20