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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
481. Changing media after transfection - When??? - From Serum containing media to serum free media (reply: 2)
482. Problem with Histag purification - (reply: 1)
483. Freezing eluted His tag protein - (reply: 5)
484. Why Ni-NTA beads became clumped after incubation? - Intensive Clumped (reply: 2)
485. His-tag protein lost it's tag - (reply: 2)
486. AnionExchange problem: proteins do not bind - (reply: 12)
487. How much protein should be loaded to the column during the purificiation? - Is less more? ;) (reply: 1)
488. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
489. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
490. Centrifugal Filter Unit - Trying to get a higher concentration. (reply: 3)
491. IMPACT system purification problem - (reply: 1)
492. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
493. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
494. How to normalize my samples before I transfect my cells - (reply: 6)
495. FBS interference in SDS PAGE - (reply: 3)
496. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
497. What make voltage not going up for the 3rd step in running IEF? - (reply: 2)
498. Help...purify and concentrate protein for IEF - (reply: 1)
499. preference of N/C terminus for tagging - (reply: 2)
500. NI-NTA purification - (reply: 2)
501. Could a 30aa peptides(3×flag+6×his) be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)
502. Ampicillin Mess-up(Experiment in progress atm) - Experiment still valid? (reply: 1)
503. RNA immunoprecipitation...HELP! - (reply: 4)
504. Dialysis - A question about buffers (reply: 6)
505. Purification problems his-tag - I have tried everything (reply: 3)
506. GST-tagged protein binds to column but is not eluted - (reply: 1)
507. urea-denatured protein precipitates after dialysis - (reply: 1)
508. Expressing 4 proteins in an operon - (reply: 6)
509. More His-tag problems - (reply: 4)
510. Problem clarifying lysate from Hi5 insect cells - (reply: 1)

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