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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
481. Adventitious chemical binding - Unwanted chemicals in protein solution (reply: 2)
482. More than one protein induced - (reply: 4)
483. Protein aggregates - Monomeric fraction varies with wavelength (reply: 1)
484. water withdrawal with PEG20.000 - Does it drag glycerol along too?-PLEASE HELP! (reply: 1)
485. Troubleshooting GST purification - (reply: 4)
486. Amount of inoculum? - for protein expression in E.coli (reply: 3)
487. BL21A or simliar - (reply: 2)
488. Role of imidazole in lysis buffer - for obtaining recombinant his-tag protein (reply: 4)
489. protein expression (temperature issue) - trying to express soluble protein at lower temperature (reply: 17)
490. Can GST-tag be separated from GST-fusion protein - (reply: 1)
491. Protein analog - (reply: 1)
492. membrane protein extraction buffer - (reply: 1)
493. What about the Baculovirus expression system? - (reply: 2)
494. Ni-NTA beads and dialysis tubing - Purification using nickel beads (reply: 4)
495. Concentrating Protein with centrifuge concentrator but 50% glycerol!!? - (reply: 2)
496. protein is degraded after purification - (reply: 2)
497. protein expression - Preparation of starter cultures (reply: 15)
498. Problem with Protein elution using GST sepharose 4B - (reply: 1)
499. how to isolate insoluble protein in bacterial induction? - (reply: 4)
500. Plasmid instability in E.coli BL21? - (reply: 3)
501. Cleaving Fusion Proteins - (reply: 1)
502. Plyse cell and DH5a - (reply: 1)
503. Quality of aliquot for SDS-page in protein purification - When is pure pure enough? (reply: 1)
504. Electroelution with no electroeluter - (reply: 3)
505. reusing Ni NTA - (reply: 2)
506. Low expression: What else can I do? - Using pET23a in BL21 PlysS (reply: 9)
507. Changing dialysis buffer every half hour and then dialysis ON - protein precipitation prevention (reply: 1)
508. Checking For Protein Expression - (reply: 3)
509. How do I get rid of this contaminant? - His-tagged protein eluted with 300mM imidazole (reply: 13)
510. Is it possible? Harsh sonificiation conditions cause degradation of my protein - (reply: 3)

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