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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
481. Centrifugal Filter Unit - Trying to get a higher concentration. (reply: 3)
482. IMPACT system purification problem - (reply: 1)
483. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
484. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
485. How to normalize my samples before I transfect my cells - (reply: 6)
486. FBS interference in SDS PAGE - (reply: 3)
487. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
488. What make voltage not going up for the 3rd step in running IEF? - (reply: 2)
489. Help...purify and concentrate protein for IEF - (reply: 1)
490. preference of N/C terminus for tagging - (reply: 2)
491. NI-NTA purification - (reply: 2)
492. Could a 30aa peptides(3×flag+6×his) be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)
493. Ampicillin Mess-up(Experiment in progress atm) - Experiment still valid? (reply: 1)
494. RNA immunoprecipitation...HELP! - (reply: 4)
495. Dialysis - A question about buffers (reply: 6)
496. Purification problems his-tag - I have tried everything (reply: 3)
497. GST-tagged protein binds to column but is not eluted - (reply: 1)
498. urea-denatured protein precipitates after dialysis - (reply: 1)
499. Expressing 4 proteins in an operon - (reply: 6)
500. More His-tag problems - (reply: 4)
501. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
502. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
503. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
504. Protein precipitates upon dialysis - (reply: 5)
505. membrane protein aggregation - (reply: 1)
506. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
507. His Tag Protein Purification - (reply: 6)
508. Amylose bound protein elution - (reply: 1)
509. hey guys,what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
510. Soluble aggregates - (reply: 3)

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