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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
481. EDTA bacterial cell lysate - (reply: 2)
482. Trouble With HIS affinity column (Ni+) binding - (reply: 1)
483. Problems with affinity purification with M2 beads - (reply: 2)
484. Expression problem using invitrogen pRSET C vector - (reply: 5)
485. Vector containing N-his tag and TEV - (reply: 1)
486. Protein expression using invitrogen pSecTag2 - (reply: 2)
487. Problems with nuclear extraction - (reply: 1)
488. His-tagged protein runs much faster than expected - 6His-Tagged CcpA protein runs around 26 kD whereas I expect to run 37 (reply: 7)
489. Removing phosphates - (reply: 2)
490. protein going to inclusion bodies - protein going to inclusion bodies (reply: 1)
491. Looking for endogenus expression by using custom antibody - (reply: 5)
492. Adventitious chemical binding - Unwanted chemicals in protein solution (reply: 2)
493. More than one protein induced - (reply: 4)
494. Protein aggregates - Monomeric fraction varies with wavelength (reply: 1)
495. water withdrawal with PEG20.000 - Does it drag glycerol along too?-PLEASE HELP! (reply: 1)
496. Troubleshooting GST purification - (reply: 4)
497. Amount of inoculum? - for protein expression in E.coli (reply: 3)
498. BL21A or simliar - (reply: 2)
499. Role of imidazole in lysis buffer - for obtaining recombinant his-tag protein (reply: 4)
500. protein expression (temperature issue) - trying to express soluble protein at lower temperature (reply: 17)
501. Can GST-tag be separated from GST-fusion protein - (reply: 1)
502. Protein analog - (reply: 1)
503. membrane protein extraction buffer - (reply: 1)
504. What about the Baculovirus expression system? - (reply: 2)
505. Ni-NTA beads and dialysis tubing - Purification using nickel beads (reply: 4)
506. Concentrating Protein with centrifuge concentrator but 50% glycerol!!? - (reply: 2)
507. protein is degraded after purification - (reply: 2)
508. protein expression - Preparation of starter cultures (reply: 15)
509. Problem with Protein elution using GST sepharose 4B - (reply: 1)
510. how to isolate insoluble protein in bacterial induction? - (reply: 4)

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