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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
481. How much protein should be loaded to the column during the purificiation? - Is less more? ;) (reply: 1)
482. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
483. Protocol for Blue Sepharose 6 Fast Flow purification - (reply: 1)
484. Centrifugal Filter Unit - Trying to get a higher concentration. (reply: 3)
485. IMPACT system purification problem - (reply: 1)
486. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
487. His tag increasing expression and solubility?! - Confusion Galore. (reply: 3)
488. How to normalize my samples before I transfect my cells - (reply: 6)
489. FBS interference in SDS PAGE - (reply: 3)
490. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
491. What make voltage not going up for the 3rd step in running IEF? - (reply: 2)
492. Help...purify and concentrate protein for IEF - (reply: 1)
493. preference of N/C terminus for tagging - (reply: 2)
494. NI-NTA purification - (reply: 2)
495. Could a 30aa peptides(3×flag+6×his) be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)
496. Ampicillin Mess-up(Experiment in progress atm) - Experiment still valid? (reply: 1)
497. RNA immunoprecipitation...HELP! - (reply: 4)
498. Dialysis - A question about buffers (reply: 6)
499. Purification problems his-tag - I have tried everything (reply: 3)
500. GST-tagged protein binds to column but is not eluted - (reply: 1)
501. urea-denatured protein precipitates after dialysis - (reply: 1)
502. Expressing 4 proteins in an operon - (reply: 6)
503. More His-tag problems - (reply: 4)
504. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
505. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
506. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
507. Protein precipitates upon dialysis - (reply: 5)
508. membrane protein aggregation - (reply: 1)
509. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
510. His Tag Protein Purification - (reply: 6)

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