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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
481. Could a 30aa peptides(3×flag+6×his) be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)
482. Ampicillin Mess-up(Experiment in progress atm) - Experiment still valid? (reply: 1)
483. RNA immunoprecipitation...HELP! - (reply: 4)
484. Dialysis - A question about buffers (reply: 6)
485. Purification problems his-tag - I have tried everything (reply: 3)
486. GST-tagged protein binds to column but is not eluted - (reply: 1)
487. urea-denatured protein precipitates after dialysis - (reply: 1)
488. Expressing 4 proteins in an operon - (reply: 6)
489. More His-tag problems - (reply: 4)
490. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
491. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
492. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
493. Protein precipitates upon dialysis - (reply: 5)
494. membrane protein aggregation - (reply: 1)
495. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
496. His Tag Protein Purification - (reply: 6)
497. Amylose bound protein elution - (reply: 1)
498. hey guys,what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
499. Soluble aggregates - (reply: 3)
500. insect protein expression - (reply: 1)
501. Can you store TNT translation products? - (reply: 1)
502. How to pack the FPLC column - (reply: 2)
503. Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
504. Sonication issues! - (reply: 3)
505. complete gene for expression? - (reply: 2)
506. buffer for membrane protein - (reply: 1)
507. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
508. Question: Bradford assay - buffer precipitates - KCl and HEPES preciptation with Bradford reagent (reply: 3)
509. membrane protein purification - (reply: 6)
510. Protein expression failing - (reply: 1)

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