Protocol Online logo
Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
481. Self-made Protease inhibitor cocktail - PMSF, EDTA, Benzamidine (reply: 4)
482. Contamination in GST fusion protein purification - (reply: 2)
483. GST-tagged proteins - (reply: 1)
484. Protino® Ni-IDA Packed Columns - Can you re-use them? (reply: 1)
485. immunoprecipation - (reply: 1)
486. Ni-NTA his tag protein purification - (reply: 1)
487. Weird Induction Problem - (reply: 5)
488. cloning of non-coding region for protein expression - protein expression (reply: 1)
489. from pet-SUMO to pet-28 - Expression problems with pet SUMO (reply: 2)
490. Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
491. Protein expression - (reply: 4)
492. EDTA bacterial cell lysate - (reply: 2)
493. Trouble With HIS affinity column (Ni+) binding - (reply: 1)
494. Problems with affinity purification with M2 beads - (reply: 2)
495. Expression problem using invitrogen pRSET C vector - (reply: 5)
496. Vector containing N-his tag and TEV - (reply: 1)
497. Protein expression using invitrogen pSecTag2 - (reply: 2)
498. Problems with nuclear extraction - (reply: 1)
499. His-tagged protein runs much faster than expected - 6His-Tagged CcpA protein runs around 26 kD whereas I expect to run 37 (reply: 7)
500. Removing phosphates - (reply: 2)
501. protein going to inclusion bodies - protein going to inclusion bodies (reply: 1)
502. Looking for endogenus expression by using custom antibody - (reply: 5)
503. Adventitious chemical binding - Unwanted chemicals in protein solution (reply: 2)
504. More than one protein induced - (reply: 4)
505. Protein aggregates - Monomeric fraction varies with wavelength (reply: 1)
506. water withdrawal with PEG20.000 - Does it drag glycerol along too?-PLEASE HELP! (reply: 1)
507. Troubleshooting GST purification - (reply: 4)
508. Amount of inoculum? - for protein expression in E.coli (reply: 3)
509. BL21A or simliar - (reply: 2)
510. Role of imidazole in lysis buffer - for obtaining recombinant his-tag protein (reply: 4)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21