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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
481. Role of imidazole in lysis buffer - for obtaining recombinant his-tag protein (reply: 4)
482. protein expression (temperature issue) - trying to express soluble protein at lower temperature (reply: 17)
483. Can GST-tag be separated from GST-fusion protein - (reply: 1)
484. Protein analog - (reply: 1)
485. membrane protein extraction buffer - (reply: 1)
486. What about the Baculovirus expression system? - (reply: 2)
487. Ni-NTA beads and dialysis tubing - Purification using nickel beads (reply: 4)
488. Concentrating Protein with centrifuge concentrator but 50% glycerol!!? - (reply: 2)
489. protein is degraded after purification - (reply: 2)
490. protein expression - Preparation of starter cultures (reply: 15)
491. Problem with Protein elution using GST sepharose 4B - (reply: 1)
492. how to isolate insoluble protein in bacterial induction? - (reply: 4)
493. Plasmid instability in E.coli BL21? - (reply: 3)
494. Cleaving Fusion Proteins - (reply: 1)
495. Plyse cell and DH5a - (reply: 1)
496. Quality of aliquot for SDS-page in protein purification - When is pure pure enough? (reply: 1)
497. Electroelution with no electroeluter - (reply: 3)
498. reusing Ni NTA - (reply: 2)
499. Low expression: What else can I do? - Using pET23a in BL21 PlysS (reply: 9)
500. Changing dialysis buffer every half hour and then dialysis ON - protein precipitation prevention (reply: 1)
501. Checking For Protein Expression - (reply: 3)
502. How do I get rid of this contaminant? - His-tagged protein eluted with 300mM imidazole (reply: 13)
503. Is it possible? Harsh sonificiation conditions cause degradation of my protein - (reply: 3)
504. Low Thrombin Cleavage Efficiency Problem - I am trying to cleave a GST tagged protein(about 35 kDa + 26 kDa GST) (reply: 1)
505. Throbin cleavage declines with older protein? - It used to work, now doesn't! (reply: 1)
506. Couple questions about GST purification - (reply: 16)
507. Immunoprecipitation - (reply: 12)
508. Newbie question: how to determine protein concentration after IP? - (reply: 8)
509. protein extraction using trizol - can we replace guanidine cholride with other denaturants? (reply: 1)
510. High pressure on Ni-NTA beads - (reply: 2)

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