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Top : New Forum Archives (2009-): : Protein-Expression-and-Purification
481. preference of N/C terminus for tagging - (reply: 2)
482. NI-NTA purification - (reply: 2)
483. Could a 30aa peptides(3×flag+6×his) be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)
484. Ampicillin Mess-up(Experiment in progress atm) - Experiment still valid? (reply: 1)
485. RNA immunoprecipitation...HELP! - (reply: 4)
486. Dialysis - A question about buffers (reply: 6)
487. Purification problems his-tag - I have tried everything (reply: 3)
488. GST-tagged protein binds to column but is not eluted - (reply: 1)
489. urea-denatured protein precipitates after dialysis - (reply: 1)
490. Expressing 4 proteins in an operon - (reply: 6)
491. More His-tag problems - (reply: 4)
492. Problem clarifying lysate from Hi5 insect cells - (reply: 1)
493. Problem with His Trap Affinity Columns (Nickel) - Column color turns - reducing of nickel? (reply: 3)
494. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
495. Protein precipitates upon dialysis - (reply: 5)
496. membrane protein aggregation - (reply: 1)
497. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
498. His Tag Protein Purification - (reply: 6)
499. Amylose bound protein elution - (reply: 1)
500. hey guys,what do you think the Anti-Flag M2 agarose beads is made of? - I want to make some homemade agarose beads,which haven't been crosslin (reply: 3)
501. Soluble aggregates - (reply: 3)
502. insect protein expression - (reply: 1)
503. Can you store TNT translation products? - (reply: 1)
504. How to pack the FPLC column - (reply: 2)
505. Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
506. Sonication issues! - (reply: 3)
507. complete gene for expression? - (reply: 2)
508. buffer for membrane protein - (reply: 1)
509. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
510. Question: Bradford assay - buffer precipitates - KCl and HEPES preciptation with Bradford reagent (reply: 3)

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