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question about start codons - (May/31/2006 )


i had a gene synthesized for me. the company that did this put a start codon at the beginning of the gene. i have subcloned this gene into a vector that has an n-terminal tag (for protein detection). my question is: will this start codon affect the inclusion of this tag in the expressed protein?
thank you!


It will just code for a standard methionine (eventually for a leucine if it's a cryptic start codon) but will not afect the "adding" of Ntag