Blue and White Colonies - (Jul/26/2006 )
I'm new to cell transformation...
Why there are white colonies surrounding the blue colonies when i using pUC19 and not the recombinant pUC19(rpUC19)?
From what i know,all the colonies will appear white when the rpUC19 was added to the plate...because the lacZ gene was being interrupted,is it?
Does the plasmid contains both the Amp gene and LacZ gene in different position?
How the lacZ gene being interrupted?
Can somebody solve my problems?
Have you ever heard of "satellites"? They usually appear around Amp resistant colonies during transformation. White colonies around blue colonies are satellites, and they are ampicillin sensitive. Because transformants break ampicillin near the colonies, ampicillin sensitive e.coli (not-transformants, competent cells) cam survive.
Why there are white colonies surrounding the blue colonies when i using pUC19 and not the recombinant pUC19(rpUC19)?
From what i know,all the colonies will appear white when the rpUC19 was added to the plate...because the lacZ gene was being interrupted,is it?
Does the plasmid contains both the Amp gene and LacZ gene in different position?
How the lacZ gene being interrupted?
Can somebody solve my problems?
That means that the white colonies are sensitive to Amp,but the transformant cells had broken the Amp,so the white colonies can survive.
I agree with the people that satelite colonies will appear after consuming the ampicillin and also these are white in colour and small in size this will happen incubation period also increase than `16hrs period