LacZ fusion with pRS415 - Protocol (Aug/05/2006 )

Im planning to clone the promoter region of my bacterial gene into the promotorless lacZ of plasmid pRS415. After doing it, i need to measure the promoter activity using beta galactosidase assay on various stages of growth. But i dont know how to recombined the promoter-lacZ fusion into my bacterial host. Ive read that i might be needing a RS88 or any bacteriophage of some sort. but im not familiar with it.Anybody who have done this, pls share your expertise. I would really appreciate it. Thanks

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What's your host?

Do you want to integrate your promoter-reporter into the genome of your host? Or can you study the activity using the plasmid?

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Hello.Thanks for your reply. My host is Salmonella typhimurium. Im confused actually whether is it necessary to integrate the promoter-reporter into the host chromosome or just study it using the plasmid. Ill be doing the beta galactosidase assay (spectro). Pls advise. Thank u so much

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