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Restriction digested plasmid - (Jul/25/2007 )

Hello Everyone,

Is the restriction enzyme digested plasmid run peculiarly in the gel (0.5%)?

-Latha Prakash-

Nope. The digested plasmid should run fine on 0.5% TAE/TBE/SB/E (choose your buffer) agarose. Perhaps your well is overloaded with DNA? Over loaded wells, can run oddly.


If the gel was not properly mixed, you could have uneven polymerisation and gel could run funny.