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Restriction digested plasmid - (Jul/25/2007 )

Hello Everyone,

Is the restriction enzyme digested plasmid run peculiarly in the gel (0.5%)?

-Latha Prakash-

Nope. The digested plasmid should run fine on 0.5% TAE/TBE/SB/E (choose your buffer) agarose. Perhaps your well is overloaded with DNA? Over loaded wells, can run oddly.

-perneseblue-

If the gel was not properly mixed, you could have uneven polymerisation and gel could run funny.

-scolix-