Restriction digested plasmid - (Jul/25/2007 )
Hello Everyone,
Is the restriction enzyme digested plasmid run peculiarly in the gel (0.5%)?
-Latha Prakash-
Nope. The digested plasmid should run fine on 0.5% TAE/TBE/SB/E (choose your buffer) agarose. Perhaps your well is overloaded with DNA? Over loaded wells, can run oddly.
-perneseblue-
If the gel was not properly mixed, you could have uneven polymerisation and gel could run funny.
-scolix-