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Sequencing Related Discussions
  1. inconsistent and low reproducibility of bisulfite sequencing - (reply: 3)
  2. Point mutation in sanger sequencing - (reply: 2)
  3. Combining alpha and beta TCR chains for sequencing - (reply: 1)
  4. Problems sequencing ligation product - (reply: 5)
  5. problem in sequencing of gene - (reply: 3)
  6. sequencing results - (reply: 4)
  7. Methods to Sequencing "Unknown" DNA - (reply: 2)
  8. Help with primer concentration for sequencing - (reply: 1)
  9. data analysis for direct sequencing BSP - (reply: 3)
  10. No sequencing of DNA methylation - (reply: 6)
  11. cDNA Library Construction by using kits and Sequencing - (reply: 1)
  12. Problem shrna cloning, bad sequencing results - (reply: 1)
  13. Sequencing gels equipment query - (reply: 2)
  14. bisulfite sequencing - (reply: 3)
  15. Detection of mutations in plasma samples using multiplex amplicon sequencing - (reply: 1)
  16. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
  17. DNA sequencing for GC rich sequence - (reply: 3)
  18. Setting up bisulfite sequencing in lab - (reply: 26)
  19. Do I need to have a biological replicate for sequencing Virus - (reply: 3)
  20. Sending a TOPO-construct for sequencing? - (reply: 1)
  21. Can ChIP sequencing be used to make exploratory findings about histone methylati - (reply: 1)
  22. PCR and sequencing of genomic DNA - (reply: 5)
  23. AZA treatment induces the genes but bisulfite sequencing does not reveal de-meth - (reply: 11)
  24. sequencing issue - (reply: 8)
  25. Sequencing of ~3,5 kb - (reply: 11)
  26. Need help on RNA preparation for RNA sequencing - (reply: 7)
  27. genome sequencing of intracellular bacterium - (reply: 1)
  28. Picking multiple clones for sequencing two copies of the same promoter - (reply: 3)
  29. sequencing basic learning - (reply: 1)
  30. PCR product sequencing - (reply: 3)
  31. Bisulfite Sequencing and PCR Troubleshooting - (reply: 2)
  32. Causes for sequencing overlap traces in one direction but not another - (reply: 4)
  33. Frameshift mutation in DNA sequencing? - (reply: 1)
  34. Extra nucleotides in sequencing... - (reply: 3)
  35. fluorescent primer vs fluorescent terminator in sequencing - (reply: 4)
  36. Quantifying bisulfite-converted DNA (pre- and post-pcr) and direct sequencing - (reply: 2)
  37. DNA sequencing - (reply: 2)
  38. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
  39. Cost of Sequencing by Post - (reply: 8)
  40. Sequencing of the cloned plasmid - (reply: 5)
  41. sequencing - (reply: 1)
  42. Low DNA concentration for sequencing - (reply: 5)
  43. miRNA sequencing from FFPE tissue - (reply: 3)
  44. Sequencing problem and restricition enzyme digestion - (reply: 9)
  45. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
  46. dscDNA synthesis suitable for Illumina Sequencing - (reply: 1)
  47. sequencing problems - (reply: 2)
  48. Sequencing RT-PCR product - (reply: 3)
  49. Bisulfite sequencing PCR worked - (reply: 5)
  50. Sanger Sequencing RNAse Interference? - (reply: 1)
  51. Sequencing Primers and Plasmids - (reply: 3)
  52. How to join two sequencing files taken form forward and reverse primer - (reply: 3)
  53. Help - Troubleshooting cloning into pET28 - all screens positive, but sequencing - (reply: 2)
  54. cDNA sequencing - (reply: 2)
  55. Help with sequencing of a 120bp PCR product. - (reply: 9)
  56. Sanger Sequencing - Capillary Lengths - (reply: 1)
  57. DNA sequencing after ligation! HELP! - (reply: 8)
  58. Sequencing of the PCR production in BSP - (reply: 1)
  59. sequencing results - (reply: 8)
  60. New to sequencing, questions about Beckman Coulter CEQ 8000 - (reply: 3)
  61. sequencing result - (reply: 2)
  62. Problem of Direct bisulfite sequencing - (reply: 1)
  63. sequencing result doesn't match my gene.... - (reply: 5)
  64. 454 sequencing - (reply: 2)
  65. Protein sequencing gaps - (reply: 1)
  66. Sequencing of PCR product - (reply: 4)
  67. sequencing result from excised DGGE bands - (reply: 3)
  68. troubleshooting sequencing results - (reply: 7)
  69. Plasmid sequencing without primers - (reply: 1)
  70. Plasmid sequencing - (reply: 1)
  71. Sequencing shows different results when sequenced from the other end - (reply: 3)
  72. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
  73. sequencing trouble - (reply: 4)
  74. dna sequencing time - (reply: 1)
  75. methods for gene sequencing - (reply: 4)
  76. Sequencing trouble - (reply: 7)
  77. Direct sequencing - BSP - (reply: 1)
  78. C/G noise in direct sequencing results - (reply: 1)
  79. statistical analysis or quantitative analysis method for bisulfite sequencing r - (reply: 4)
  80. COL E1 mutations in vector after sequencing - (reply: 1)
  81. check points while preparing for sequencing - (reply: 10)
  82. wrong size in agarose gel, right size in sequencing - (reply: 7)
  83. Bisulfite treatment of DNA follow by Sequencing - (reply: 2)
  84. Direct sequencing PCR product - for bisulfite sequencing (reply: 3)
  85. Plasmid sequencing failed why? - (reply: 10)
  86. sequencing problem - (reply: 1)
  87. transcriptome sequencing help - (reply: 2)
  88. Cleaning up RT-PCR product before sequencing - (reply: 1)
  89. Is pyrosequencing necessary? - Sequencing PCR products (reply: 2)
  90. Amount of DNA to send for sequencing - linear response in signal intenstiy? - (reply: 1)
  91. Cycle sequencing (Big Dye) - (reply: 12)
  92. Sequencing miRNA qRT-PCR Products - (reply: 5)
  93. Sequencing of 18srRNA - (reply: 2)
  94. Deep sequencing adaptor question - (reply: 1)
  95. Direct PCR Sequencing of BS Products - Having trouble (reply: 1)
  96. pri-miRNA sequencing - (reply: 4)
  97. Sequencing analysis for detection of mutations - (reply: 2)
  98. Drop down during bisulfite sequencing - (reply: 2)
  99. Do you know good sequencing companies? - Sanger sequencing (reply: 15)
  100. Illumina sample prep for Multiplexed sequencing... - How does it work? (reply: 3)
  101. Bisulfite sequencing primer design query (probably simple) - (reply: 4)
  102. PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
  103. Sequencing promoter regions - (reply: 6)
  104. difference between PCR primers and sequencing primers - (reply: 1)
  105. Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
  106. Really strange problem with sequencing and plasmid purification! - (reply: 8)
  107. Can sequencing determine carrier status???? - Something I should have thought of before......... (reply: 2)
  108. Amplification of bisulfite converted gDNA for sequencing - (reply: 1)
  109. Sequencing mistake - (reply: 3)
  110. New to sequencing, primer design - (reply: 2)
  111. protein sequencing company - (reply: 2)
  112. pcr / primer theory in bisulfite sequencing - (reply: 4)
  113. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
  114. trouble in TOPO TA sequencing - (reply: 1)
  115. Feel really stupid for asking this ...Bisulfite Sequencing, help please! - (reply: 10)
  116. sequencing trace question - (reply: 1)
  117. Bisulfite sequencing is giving problem - (reply: 7)
  118. DNA sequencing question help - make up sequencing mastermix (reply: 1)
  119. Newbie to Sequencing Question - (reply: 1)
  120. help analyzing a sequencing trace - double peaks, then clean trace? (reply: 5)
  121. Miniprep and sequencing - (reply: 4)
  122. DNA Sequencing / Cloning ? - (reply: 5)
  123. Bisulfite sequencing - (reply: 3)
  124. electrophoresis/ difference between expected size and result/ what's the alt - How to confirm the band means the expected DNA? Sequencing&what else? (reply: 5)
  125. dna sequencing - (reply: 1)
  126. Help Help, sequencing not working - Sequencing-noisy (reply: 4)
  127. Bisulfite sequencing not working! - (reply: 4)
  128. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  129. Basic DNA sequencing question - (reply: 5)
  130. Can normal sequencing services sequence >1kb product? - sequencing (reply: 1)
  131. How to clean-up 96-well microplates? - to re-use 96-well plates for PCR and sequencing (reply: 4)
  132. Problems with sequencing - (reply: 5)
  133. Help with data from directly sequencing BSP product... - (reply: 2)
  134. sequencing question help! - urgent! (reply: 2)
  135. Urgent! Sequencing a mixture of ssDNA fragments of the same gene - ssDNA different in size amplified from a single primer (reply: 2)
  136. Deep sequencing to find gene targets of protein? - (reply: 1)
  137. Bisulfite sequencing PCR not working - (reply: 5)
  138. bisulfite sequencing cloning problem (methylation of DNa) - bisulfite sequencing cloning problem (methylation of DNa) (reply: 3)
  139. Removing sequencing migration artefacts - (reply: 1)
  140. Sequencing of gene promoter/terminator, when you only have the AA sequence of th - (reply: 2)
  141. N-Terminal Sequencing - (reply: 2)
  142. primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
  143. Direct sequencing of Bisulphite PCR product - (reply: 1)
  144. How to measure genome size? - Before sequencing an eukarotic genome, it needs to know the size (reply: 1)
  145. Strange Sequencing results - (reply: 4)
  146. question about bigdye sequencing reaction & capillary electrophoresis - (reply: 3)
  147. Sequencing - (reply: 1)
  148. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
  149. sequencing with forward/reverse primers - (reply: 7)
  150. Bisulfite Sequencing PCR help! - BSP is Failing like Gangbusters. Please help! (reply: 6)
  151. N-terminal sequencing - (reply: 2)
  152. sequencing the reverse strand - (reply: 2)
  153. qestion about sequencing - (reply: 2)
  154. Storage of sequencing files - (reply: 1)
  155. DNA methylation analysis - direct sequencing gives CT peaks - (reply: 1)
  156. sequencing primer: T7 or T7promoter? - (reply: 4)
  157. Free ChIP sequencing analysis software? - (reply: 2)
  158. what primers should I use for DNA sequencing? please help - (reply: 2)
  159. what primers should I use for DNA sequencing?? - (reply: 3)
  160. sequencing clones after BSP : need miniprep ?? - (reply: 1)
  161. Viral genome sequencing - (reply: 10)
  162. Sequencing different colonies - (reply: 5)
  163. Sequencing - identifying backbone and deciphering insert information - (reply: 8)
  164. RNA, mRNA, sequencing... HELP - (reply: 2)
  165. Sequencing doubts - (reply: 3)
  166. Perplexing Bisulfite Sequencing Problem - (reply: 3)
  167. What would happen? sequencing question - (reply: 2)
  168. PCR, followed by sequencing... - why ?? (reply: 3)
  169. length of bisulfite sequencing reads on a ABI - my reads are not so long!!! (reply: 2)
  170. prediction of novel microRNAs from deep sequencing data? - microRNA deep sequencing (reply: 1)
  171. Strange results from bisulfite sequencing - (reply: 2)
  172. Sequencing results failed - (reply: 6)
  173. What do people use as sequencing control in ChIP-Seq - (reply: 11)
  174. Bisulfite Sequencing PCR - (reply: 4)
  175. Sequencing results analysis - (reply: 1)
  176. Sequencing - (reply: 2)
  177. Not getting good signal in sequencing - (reply: 2)
  178. GC rich DNA sequencing - (reply: 3)
  179. Direct sequencing of BSP product - (reply: 3)
  180. sequencing storage question - (reply: 2)
  181. Sequencing with ABI Prism 310 - capilares problem (reply: 1)
  182. deep sequencing miRNA - how to analyse the miRNA deep sequencing data (reply: 2)
  183. ESME software for analysis of bisulphite sequencing traces - Where can I get the full version? (reply: 2)
  184. Sequencing of scarce real-time amplicons - (reply: 1)
  185. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  186. Bisulfite genomic sequencing PCR - PCR conditions (reply: 7)
  187. comparison of bisulfite sequencing and base specific cleavage coupled with Mass - (reply: 5)
  188. Analysis of direct bisulfite sequencing data - (reply: 9)
  189. WHich primer to use for sequencing - (reply: 5)
  190. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
  191. primers fpr sequencing - (reply: 4)
  192. GENOMIC PCR - PCR PRODUCT FOR SEQUENCING (reply: 4)
  193. Sequencing very short (60-80bp) amplicons ? - (reply: 3)
  194. sequencing problem after pcr - (reply: 4)
  195. Capillary DNA Sequencing - (reply: 6)
  196. problem in sequencing - (reply: 4)
  197. DNA sequencing analysis - DNA sequencing analysis (reply: 1)
  198. DNA sequencing analysis - DNA sequencing analysis (reply: 11)
  199. sequencing the pcr product - (reply: 15)