Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Sequencing Related Discussions
  1. Sequencing virus from liver samples - (reply: 1)
  2. inconsistent and low reproducibility of bisulfite sequencing - (reply: 3)
  3. Point mutation in sanger sequencing - (reply: 2)
  4. Combining alpha and beta TCR chains for sequencing - (reply: 1)
  5. Cleaning loading dye for PCR sequencing - (reply: 1)
  6. Problems sequencing ligation product - (reply: 5)
  7. problem in sequencing of gene - (reply: 3)
  8. sequencing results - (reply: 4)
  9. Methods to Sequencing "Unknown" DNA - (reply: 2)
  10. Help with primer concentration for sequencing - (reply: 1)
  11. data analysis for direct sequencing BSP - (reply: 3)
  12. No sequencing of DNA methylation - (reply: 6)
  13. cDNA Library Construction by using kits and Sequencing - (reply: 1)
  14. Problem shrna cloning, bad sequencing results - (reply: 1)
  15. Sequencing gels equipment query - (reply: 2)
  16. bisulfite sequencing - (reply: 3)
  17. Detection of mutations in plasma samples using multiplex amplicon sequencing - (reply: 1)
  18. sequencing getting wrong 1 nucleotide :((( - (reply: 4)
  19. DNA sequencing for GC rich sequence - (reply: 3)
  20. Setting up bisulfite sequencing in lab - (reply: 26)
  21. Do I need to have a biological replicate for sequencing Virus - (reply: 3)
  22. Sending a TOPO-construct for sequencing? - (reply: 1)
  23. Can ChIP sequencing be used to make exploratory findings about histone methylati - (reply: 1)
  24. PCR and sequencing of genomic DNA - (reply: 5)
  25. AZA treatment induces the genes but bisulfite sequencing does not reveal de-meth - (reply: 11)
  26. sequencing issue - (reply: 8)
  27. Sequencing of ~3,5 kb - (reply: 11)
  28. Need help on RNA preparation for RNA sequencing - (reply: 7)
  29. genome sequencing of intracellular bacterium - (reply: 1)
  30. Picking multiple clones for sequencing two copies of the same promoter - (reply: 3)
  31. sequencing basic learning - (reply: 1)
  32. PCR product sequencing - (reply: 3)
  33. Bisulfite Sequencing and PCR Troubleshooting - (reply: 2)
  34. Causes for sequencing overlap traces in one direction but not another - (reply: 4)
  35. Frameshift mutation in DNA sequencing? - (reply: 1)
  36. Extra nucleotides in sequencing... - (reply: 3)
  37. fluorescent primer vs fluorescent terminator in sequencing - (reply: 4)
  38. Quantifying bisulfite-converted DNA (pre- and post-pcr) and direct sequencing - (reply: 2)
  39. DNA sequencing - (reply: 2)
  40. Will long exposures of DNA to UV (302nm) alter sequencing data? - (reply: 4)
  41. Cost of Sequencing by Post - (reply: 8)
  42. Sequencing of the cloned plasmid - (reply: 5)
  43. sequencing - (reply: 1)
  44. Low DNA concentration for sequencing - (reply: 5)
  45. miRNA sequencing from FFPE tissue - (reply: 3)
  46. Sequencing problem and restricition enzyme digestion - (reply: 9)
  47. How do I know the orientation of my insert in a plasmid after sequencing? - (reply: 2)
  48. dscDNA synthesis suitable for Illumina Sequencing - (reply: 1)
  49. sequencing problems - (reply: 2)
  50. Sequencing RT-PCR product - (reply: 3)
  51. Bisulfite sequencing PCR worked - (reply: 5)
  52. Sanger Sequencing RNAse Interference? - (reply: 1)
  53. Sequencing Primers and Plasmids - (reply: 3)
  54. How to join two sequencing files taken form forward and reverse primer - (reply: 3)
  55. Help - Troubleshooting cloning into pET28 - all screens positive, but sequencing - (reply: 2)
  56. cDNA sequencing - (reply: 2)
  57. Help with sequencing of a 120bp PCR product. - (reply: 9)
  58. Sanger Sequencing - Capillary Lengths - (reply: 1)
  59. DNA sequencing after ligation! HELP! - (reply: 8)
  60. Sequencing of the PCR production in BSP - (reply: 1)
  61. sequencing results - (reply: 8)
  62. New to sequencing, questions about Beckman Coulter CEQ 8000 - (reply: 3)
  63. sequencing result - (reply: 2)
  64. Problem of Direct bisulfite sequencing - (reply: 1)
  65. sequencing result doesn't match my gene.... - (reply: 5)
  66. 454 sequencing - (reply: 2)
  67. Protein sequencing gaps - (reply: 1)
  68. Sequencing of PCR product - (reply: 4)
  69. sequencing result from excised DGGE bands - (reply: 3)
  70. troubleshooting sequencing results - (reply: 7)
  71. Plasmid sequencing without primers - (reply: 1)
  72. Plasmid sequencing - (reply: 1)
  73. Sequencing shows different results when sequenced from the other end - (reply: 3)
  74. plasmid sequencing problem from single colony (mixed sequencing) - (reply: 3)
  75. sequencing trouble - (reply: 4)
  76. dna sequencing time - (reply: 1)
  77. methods for gene sequencing - (reply: 4)
  78. Sequencing trouble - (reply: 7)
  79. Direct sequencing - BSP - (reply: 1)
  80. C/G noise in direct sequencing results - (reply: 1)
  81. statistical analysis or quantitative analysis method for bisulfite sequencing r - (reply: 4)
  82. COL E1 mutations in vector after sequencing - (reply: 1)
  83. check points while preparing for sequencing - (reply: 10)
  84. wrong size in agarose gel, right size in sequencing - (reply: 7)
  85. Bisulfite treatment of DNA follow by Sequencing - (reply: 2)
  86. Direct sequencing PCR product - for bisulfite sequencing (reply: 3)
  87. Plasmid sequencing failed why? - (reply: 10)
  88. sequencing problem - (reply: 1)
  89. transcriptome sequencing help - (reply: 2)
  90. Cleaning up RT-PCR product before sequencing - (reply: 1)
  91. Is pyrosequencing necessary? - Sequencing PCR products (reply: 2)
  92. Amount of DNA to send for sequencing - linear response in signal intenstiy? - (reply: 1)
  93. Cycle sequencing (Big Dye) - (reply: 12)
  94. Sequencing miRNA qRT-PCR Products - (reply: 5)
  95. Sequencing of 18srRNA - (reply: 2)
  96. Deep sequencing adaptor question - (reply: 1)
  97. Direct PCR Sequencing of BS Products - Having trouble (reply: 1)
  98. pri-miRNA sequencing - (reply: 4)
  99. Sequencing analysis for detection of mutations - (reply: 2)
  100. Drop down during bisulfite sequencing - (reply: 2)
  101. Do you know good sequencing companies? - Sanger sequencing (reply: 15)
  102. Illumina sample prep for Multiplexed sequencing... - How does it work? (reply: 3)
  103. Bisulfite sequencing primer design query (probably simple) - (reply: 4)
  104. PCR for sequencing genomic DNA (multiple alleles) - Are there preferences for amplification of certain sequences? (reply: 1)
  105. Sequencing promoter regions - (reply: 6)
  106. difference between PCR primers and sequencing primers - (reply: 1)
  107. Forward primer not working during DNA sequencing - DNA sequencing (reply: 10)
  108. Really strange problem with sequencing and plasmid purification! - (reply: 8)
  109. Can sequencing determine carrier status???? - Something I should have thought of before......... (reply: 2)
  110. Amplification of bisulfite converted gDNA for sequencing - (reply: 1)
  111. Sequencing mistake - (reply: 3)
  112. New to sequencing, primer design - (reply: 2)
  113. protein sequencing company - (reply: 2)
  114. pcr / primer theory in bisulfite sequencing - (reply: 4)
  115. Sequencing - why are left-over PCR primers an issue? - (reply: 3)
  116. trouble in TOPO TA sequencing - (reply: 1)
  117. Feel really stupid for asking this ...Bisulfite Sequencing, help please! - (reply: 10)
  118. sequencing trace question - (reply: 1)
  119. Bisulfite sequencing is giving problem - (reply: 7)
  120. DNA sequencing question help - make up sequencing mastermix (reply: 1)
  121. Newbie to Sequencing Question - (reply: 1)
  122. help analyzing a sequencing trace - double peaks, then clean trace? (reply: 5)
  123. Miniprep and sequencing - (reply: 4)
  124. DNA Sequencing / Cloning ? - (reply: 5)
  125. Bisulfite sequencing - (reply: 3)
  126. electrophoresis/ difference between expected size and result/ what's the alt - How to confirm the band means the expected DNA? Sequencing&what else? (reply: 5)
  127. dna sequencing - (reply: 1)
  128. Help Help, sequencing not working - Sequencing-noisy (reply: 4)
  129. Bisulfite sequencing not working! - (reply: 4)
  130. PgemT-Easy sequencing primers, please rate - I am deciding which primer pairs to use for cloning (reply: 2)
  131. Basic DNA sequencing question - (reply: 5)
  132. Can normal sequencing services sequence >1kb product? - sequencing (reply: 1)
  133. How to clean-up 96-well microplates? - to re-use 96-well plates for PCR and sequencing (reply: 4)
  134. Problems with sequencing - (reply: 5)
  135. Help with data from directly sequencing BSP product... - (reply: 2)
  136. sequencing question help! - urgent! (reply: 2)
  137. Urgent! Sequencing a mixture of ssDNA fragments of the same gene - ssDNA different in size amplified from a single primer (reply: 2)
  138. Deep sequencing to find gene targets of protein? - (reply: 1)
  139. Bisulfite sequencing PCR not working - (reply: 5)
  140. bisulfite sequencing cloning problem (methylation of DNa) - bisulfite sequencing cloning problem (methylation of DNa) (reply: 3)
  141. Removing sequencing migration artefacts - (reply: 1)
  142. Sequencing of gene promoter/terminator, when you only have the AA sequence of th - (reply: 2)
  143. N-Terminal Sequencing - (reply: 2)
  144. primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
  145. Direct sequencing of Bisulphite PCR product - (reply: 1)
  146. How to measure genome size? - Before sequencing an eukarotic genome, it needs to know the size (reply: 1)
  147. Strange Sequencing results - (reply: 4)
  148. question about bigdye sequencing reaction & capillary electrophoresis - (reply: 3)
  149. Sequencing - (reply: 1)
  150. How to amplify unknown cDNA sequences from a RIP for sequencing - (reply: 1)
  151. sequencing with forward/reverse primers - (reply: 7)
  152. Bisulfite Sequencing PCR help! - BSP is Failing like Gangbusters. Please help! (reply: 6)
  153. N-terminal sequencing - (reply: 2)
  154. sequencing the reverse strand - (reply: 2)
  155. qestion about sequencing - (reply: 2)
  156. Storage of sequencing files - (reply: 1)
  157. DNA methylation analysis - direct sequencing gives CT peaks - (reply: 1)
  158. sequencing primer: T7 or T7promoter? - (reply: 4)
  159. Free ChIP sequencing analysis software? - (reply: 2)
  160. what primers should I use for DNA sequencing? please help - (reply: 2)
  161. what primers should I use for DNA sequencing?? - (reply: 3)
  162. sequencing clones after BSP : need miniprep ?? - (reply: 1)
  163. Viral genome sequencing - (reply: 10)
  164. Sequencing different colonies - (reply: 5)
  165. Sequencing - identifying backbone and deciphering insert information - (reply: 8)
  166. RNA, mRNA, sequencing... HELP - (reply: 2)
  167. Sequencing doubts - (reply: 3)
  168. Perplexing Bisulfite Sequencing Problem - (reply: 3)
  169. What would happen? sequencing question - (reply: 2)
  170. PCR, followed by sequencing... - why ?? (reply: 3)
  171. length of bisulfite sequencing reads on a ABI - my reads are not so long!!! (reply: 2)
  172. prediction of novel microRNAs from deep sequencing data? - microRNA deep sequencing (reply: 1)
  173. Strange results from bisulfite sequencing - (reply: 2)
  174. Sequencing results failed - (reply: 6)
  175. What do people use as sequencing control in ChIP-Seq - (reply: 11)
  176. Bisulfite Sequencing PCR - (reply: 4)
  177. Sequencing results analysis - (reply: 1)
  178. Sequencing - (reply: 2)
  179. Not getting good signal in sequencing - (reply: 2)
  180. GC rich DNA sequencing - (reply: 3)
  181. Direct sequencing of BSP product - (reply: 3)
  182. sequencing storage question - (reply: 2)
  183. Sequencing with ABI Prism 310 - capilares problem (reply: 1)
  184. deep sequencing miRNA - how to analyse the miRNA deep sequencing data (reply: 2)
  185. ESME software for analysis of bisulphite sequencing traces - Where can I get the full version? (reply: 2)
  186. Sequencing of scarce real-time amplicons - (reply: 1)
  187. sequencing result for primer designing - sequnce hit primer or gene? (reply: 1)
  188. Bisulfite genomic sequencing PCR - PCR conditions (reply: 7)
  189. comparison of bisulfite sequencing and base specific cleavage coupled with Mass - (reply: 5)
  190. Analysis of direct bisulfite sequencing data - (reply: 9)
  191. WHich primer to use for sequencing - (reply: 5)
  192. New Technologies Real time PCR, cloning, microarray, sequencing - (reply: 3)
  193. primers fpr sequencing - (reply: 4)
  194. GENOMIC PCR - PCR PRODUCT FOR SEQUENCING (reply: 4)
  195. Sequencing very short (60-80bp) amplicons ? - (reply: 3)
  196. sequencing problem after pcr - (reply: 4)
  197. Capillary DNA Sequencing - (reply: 6)
  198. problem in sequencing - (reply: 4)
  199. DNA sequencing analysis - DNA sequencing analysis (reply: 1)
  200. DNA sequencing analysis - DNA sequencing analysis (reply: 11)
  201. sequencing the pcr product - (reply: 15)