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What would happen? sequencing question - (Aug/18/2009 )

What would happen if a sequencing reaction was left O/N in the PCR block at RT? Would this affect the trace?

-Kami23-

Kami23 on Aug 18 2009, 05:38 PM said:

What would happen if a sequencing reaction was left O/N in the PCR block at RT? Would this affect the trace?

Considering that I leave my sequencing products ON at 15 C without observing any decrease in trace quality, I think it shouldn't really matter. Might of course be dependent on your used chemicals, I use ABI's BigDye Terminator 1.1 for reference.

-Timmay-

Timmay on Aug 18 2009, 04:52 PM said:

Kami23 on Aug 18 2009, 05:38 PM said:

What would happen if a sequencing reaction was left O/N in the PCR block at RT? Would this affect the trace?

Considering that I leave my sequencing products ON at 15 C without observing any decrease in trace quality, I think it shouldn't really matter. Might of course be dependent on your used chemicals, I use ABI's BigDye Terminator 1.1 for reference.



Ok its using big Dye 3.1 if that helps :)

-Kami23-