Sequencing with ABI Prism 310 - capilares problem (Jul/06/2009 )
We are using in our lab ABI Prism 310 Genetic Analyzer and I want to ask about capilares. ABI offer two types of capilares: 47cm for POP-4 polymer and 61cm for POP-6 polymer. For me most important thing is that I want to read first 300 nt of DNAs. Which capilares have better read resolution for the first 300 nucleotides?
Anyone can help me with this?
You should use the short capillary, which will speed up your run. They will perform similarly for the first 500 bp of sequencing. You need not switch to POP4, if you already have POP6.