qestion about sequencing - (Nov/17/2009 )

Hi,

First sorry if it's not the proper place to post this but I didn't know where to put it so...
My question is, when you send samples to sequence, they always ask for the kind of vector you used. Why?
I mean, I'm sending them my plasmid, and the primers so why do they need to know what kind of vector I used?

I'm asking because one of the student made a mistake and didn't write down the right vector. So is it really important? Does she absolutely need to change it or should it be ok?

thanks

-selaphiel-

Can't you just call the company and make sure they use your primers?

-Maddie-

selaphiel on Nov 17 2009, 12:47 PM said:

Well, I don't really know but it seems that they may use the vector type to cleave and/or start sequencing the dna at a concrete site or as a sequencing control.

-Feelcontraire-