Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
MicroRNA Related Discussions
  1. miRNA-meditated miRNA suppression? - (reply: 1)
  2. miRNA yield from human urine - (reply: 2)
  3. Sperm microRNA isolation - (reply: 2)
  4. about microrna overexpressing - (reply: 2)
  5. BLOCK-iT Pol II miR RNAi system: Issues with generating stable knockdown - (reply: 1)
  6. prediction of microRNA targets using the sequence - (reply: 1)
  7. commercially available miRNA or small RNA? - (reply: 2)
  8. qPCR and miRNA isolation - (reply: 4)
  9. Gene silencing and miRNA isolation - (reply: 1)
  10. Reference genes in miRNAs - (reply: 3)
  11. Pan-Cancer pattern of lncRNAs, miRNAs and their regulatory networks - (reply: 2)
  12. Internal control for miRNA RT-PCR - (reply: 1)
  13. miRNA knockdown in a mouse, tail vein injection (~50% KD) - (reply: 2)
  14. Question: How to differentiate siRNAs and mature miRNAs in plant by Northern blo - (reply: 3)
  15. synthetic microRNA stability - (reply: 1)
  16. scrambeled miR sequence - (reply: 5)
  17. miRNA overexpression troubleshooting - (reply: 2)
  18. overexpress mature microRNA by siRNA vector - (reply: 2)
  19. MiRNA obtaining - (reply: 1)
  20. miRNA sequencing from FFPE tissue - (reply: 3)
  21. which to use: miR or miRexpression vector - (reply: 1)
  22. Detecting miRNA mimics by RT-PCR? - (reply: 4)
  23. micro RNA - (reply: 1)
  24. Overexpressing miR - rescue by co-overexpressing its target - (reply: 1)
  25. miRNA quantification - (reply: 3)
  26. MicroRNA nomenclature - (reply: 4)
  27. microRNA promoter based expression vector? - (reply: 1)
  28. miRNA expression profiling - (reply: 3)
  29. microRNA validation in vivo - (reply: 1)
  30. Synthetic microrna for normalization in serum samples - (reply: 1)
  31. miRNA qPCR normalization/control gene sequence!!!! - (reply: 2)
  32. What is the best way to overexpress miRNAs? - (reply: 1)
  33. Looking for tool that searches a list of genes for common MiRs - (reply: 1)
  34. validation of microRNA target gene - (reply: 6)
  35. Discrepancies between miRNA Array Data and PCR Data - (reply: 1)
  36. Can I use custom made synthetic miRNA for Overexpression? - (reply: 1)
  37. question about universal and specific primers for miRNA real time pcr - (reply: 1)
  38. miRNA from plasma_purity problem after RNA isolation - (reply: 1)
  39. Substituting miRNA with ODNs for testing efficiency of dendrimers...? - (reply: 4)
  40. microRNA exiqon individual assays - (reply: 1)
  41. Is it posssible using shRNA vector to generate microRNA? - (reply: 2)
  42. miRNA extraction from cell-conditioned media? - (reply: 1)
  43. microRNA and 3' UTR - (reply: 2)
  44. Designing primers for miRNA located on minus strand. - (reply: 2)
  45. Need help with extracting miRNA from human serum using mirVana kit - (reply: 2)
  46. checking whther there is any miRNA in introns of a specific gene. - (reply: 1)
  47. Guessing candidate miRNAs from mRNA microarray - (reply: 2)
  48. miRNA in serum - (reply: 1)
  49. How to predict targets from a novel miRNA - (reply: 1)
  50. Let-7 and miR-143 miRNA? - (reply: 1)
  51. Targets of mirna* - (reply: 1)
  52. Bioinformatic tools for novel miRNA target prediction - (reply: 2)
  53. How to predict targets of unkown miRNAs in Drosophila - (reply: 2)
  54. placing siRNA seq into miRNA stem-loop - (reply: 4)
  55. How many RNA I need for detecting plasma miR expression? - (reply: 1)
  56. problem with miRNA functional study - (reply: 1)
  57. how does the binding of miRNA at 3'UTR regulates the gene - (reply: 3)
  58. Promoter activity of microRNA - (reply: 3)
  59. renilla values are going down with firefly values after miRNA transfection - (reply: 1)
  60. Which miRNA probe to choose? - (reply: 1)
  61. miRNA knockdown experiment controls - Very confused (reply: 2)
  62. miRNA cloning - (reply: 2)
  63. Help on mIcroRNA expression levels - concentration needed (reply: 1)
  64. miRNA microarray using TLDA - (reply: 1)
  65. miRNA from serum - (reply: 1)
  66. Triage of miRNA targets by qPCR - (reply: 1)
  67. miRNA knockdown approaches - (reply: 6)
  68. miRNA/siRNA transfection doubts - (reply: 2)
  69. Sequencing miRNA qRT-PCR Products - (reply: 5)
  70. PCR of microRNA first strand cDNA - (reply: 1)
  71. How to express a rare microRNA - (reply: 2)
  72. miRNA LNA - how can I designed a scramble ? (reply: 1)
  73. 3'UTR luciferase assay with 5 miRNA target sites - how to design 5 miRNA target sites in the 3'UTR (reply: 2)
  74. diffrence between miRNA family and miRNA cluster - diefinitions of microRNA cluster and microRNA family (reply: 1)
  75. miRNA increases transcription - Know of any publications where a miRNA induces transcription? (reply: 3)
  76. miRNA prediction on a given mRNA - (reply: 5)
  77. microRNA knockdown its target by western blot but can't inhibit the 3'UT - want a reliable protocol to do the 3'UTR luciferase assay (reply: 3)
  78. Help with real-time PCR quantification of miRNA - (reply: 8)
  79. Low miRNA expression? - (reply: 1)
  80. Problems with amplifying microRNA - (reply: 3)
  81. mirna overexpression/ library versus single construct/ unspecific pcr - unspecific band when pcr on gDNA from library but not on gDNA fron sin (reply: 2)
  82. miRNA isolation kit - enquiry for kit for miRNA isolation from CSF (reply: 3)
  83. Need help - How we can design primer for miRNA (reply: 1)
  84. problem in reporducing my result!!! - miRNA expression (reply: 1)
  85. Lentivirus-based Knockdown for miRNAs? - for hard-to-transfect cells (reply: 10)
  86. How about Exiqon miRCURY miRNA PCR system? - (reply: 2)
  87. How to overexpress miRs - (reply: 1)
  88. Silencing of a miRNA family - (reply: 1)
  89. miRNA overexpression for target validation - (reply: 1)
  90. MiRNA detection and info - An research on MiRNA detection (reply: 5)
  91. transfecting cells with mature miRNA or precursor miRNAs? - (reply: 5)
  92. microRNA functional classification - (reply: 1)
  93. miRNAs and antibiotics - (reply: 1)
  94. Obtaining 3'UTR and perform microRNA target prediction - How to commence? (reply: 2)
  95. can you please help me to design primers for reverse transcription of miRNA and - miRNA primers (reply: 2)
  96. miRNA spike-in control - (reply: 6)
  97. miRNA 3ŽUTR target expression vectors - (reply: 6)
  98. Can miRNAs regulate miRNA expression - (reply: 3)
  99. miRNA directed upregulation?! - (reply: 3)
  100. microRNA profiling using ABI taqman low density microRNA array (TLDA) - (reply: 1)
  101. how to expression mirna in a specific tissue ? - (reply: 1)
  102. the necessity of microRNA mutation design - (reply: 1)
  103. How to retrieve precursor miRNA sequence with extra bases for cloning - (reply: 4)
  104. miRNA clusters - miR-19 (reply: 1)
  105. mir permanent target, - general theory (reply: 1)
  106. MiRNA mediates haemodynamic-to-Vegf signalling in angiogenesis - Link to journal (reply: 3)
  107. Illumina microRNA expression arrays - Problems with clustering different experiments...HELP! (reply: 5)
  108. lentiviral overexpression of miRNAs - (reply: 6)
  109. miRNA expression - (reply: 2)
  110. Problem with validation of the miRNA binding site in 3' UTR reporter - (reply: 4)
  111. detect miRNAs expression by qPCR - (reply: 2)
  112. quantification of the expression of pre-miRNA and miRNA in plants - (reply: 1)
  113. How many miRNA will be down regulated after knocking down Dicer in mouse - (reply: 3)
  114. Can anybody help me with miRanda tool for miRNA prediction - (reply: 1)
  115. miRNA isolation from serum - (reply: 12)
  116. DNA and salt contamination using mirVANa miRNA isolation kit - (reply: 6)
  117. Bands in a miRNA gel - (reply: 1)
  118. multiplexing taqman miRNA reverse transcription - (reply: 2)
  119. miRNA isolation - (reply: 3)
  120. miRNA northern - (reply: 1)
  121. Half-life of Transfected pre-miRNA and miRNA inhibitors - (reply: 2)
  122. miRNA promoter - (reply: 7)
  123. The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m - (reply: 8)
  124. microrna quantitation ---use northern blot or qrt-pcr? - (reply: 1)
  125. Lentivial Knockdown of miRNAs - (reply: 8)
  126. total RNA (for miRNA) extraction from human serum - (reply: 11)
  127. microRNA database - in both animals and plant (reply: 1)
  128. Choosing the right refrence sample for microRNA array - (reply: 3)
  129. how do I know if there is a miRNA modulating expression? - identification of miRNA in my system (reply: 3)
  130. One miRNA modulating expression of about 600 genes - Link to paper (reply: 1)
  131. miRNA gene UTRs? - (reply: 3)
  132. Pronounciation of miRNA - (reply: 2)
  133. miRNA TARGETS - (reply: 4)
  134. Concatemerization of miRNA... - (reply: 1)
  135. Co-transfection of miRNA and vector expressing target sequence - (reply: 2)
  136. How to overexpress star miRNA (miRNA*) from pre-miRNA construct - (reply: 4)
  137. miRNA and TRIZOL - (reply: 7)
  138. Who are the big names in miRNA research? - (reply: 5)
  139. MiRna - How to screen the MiRNA Targets (reply: 2)
  140. mature star miRNA - (reply: 2)
  141. Inhibition of miRNA doesn't enhance its target expression - (reply: 4)
  142. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 1)
  143. miScript (Qiagen) - RT of both miRNA and mRNA - does it work? (reply: 4)
  144. how to test if a given microRNA regulates a gene of interest - microrna target (reply: 7)
  145. How to batch search my microRNA accession numbers? - (reply: 2)
  146. miR family - (reply: 1)
  147. ABI Taqman Low density microRNA Array card - (reply: 3)
  148. novel microRNA prediction - (reply: 2)
  149. translation of cleaved miRNA targets - (reply: 3)
  150. prediction of novel microRNAs from deep sequencing data? - microRNA deep sequencing (reply: 1)
  151. how to choose ready-made or customize miRNA microarray chip - (reply: 1)
  152. ready made or customize miRNA microarray chip - (reply: 2)
  153. miRNA isolation from liquid samples - (reply: 18)
  154. Why the fluoresence is dim or dispear in miRNA vector? - miRNA (reply: 3)
  155. miRNA mimics - (reply: 2)
  156. deep sequencing miRNA - how to analyse the miRNA deep sequencing data (reply: 2)
  157. about the microRNA seqence database - (reply: 3)
  158. miRNA Arrays - which to use? - (reply: 2)
  159. Got miRNA, need targets? - Link 'em and sink 'em! (reply: 1)
  160. Knockdown of miRNAs - (reply: 8)
  161. endogenous miRNA of cos-7 cell - (reply: 2)
  162. Controls for Overexpressing miRNAs - (reply: 2)
  163. miRNA real time PCR by SYBR green methods - (reply: 1)
  164. cloning miRNA library - (reply: 4)
  165. Probe size for miRNA Northern blot? - (reply: 8)
  166. miRNA Northern Blot gel - (reply: 4)
  167. How to design probes for microRNA Northern Blot - (reply: 4)
  168. miRNA turnover rate - how long are endogenous miRs 'viable' (reply: 1)
  169. To Fizban: miRNA validation - (reply: 5)
  170. SYBR Green miRNA analysis - (reply: 1)
  171. Ncode miRNA RT & qPCR system - can mRNA be reverse transcribed in same RT reaction? (reply: 4)
  172. denature RNA and miRNA Q-PCR - (reply: 1)
  173. Old threads on "siRNA, microRNA and RNAi" are achived here - (reply: 3)
  174. Identification of miRNA and siRNA - (reply: 1)