Can I use custom made synthetic miRNA for Overexpression? - (Jul/30/2012 )
Yes, you can design your own miRNA mimics and use it to overexpress the miRNA. You can order as duplex RNA, but the duplex may not be perfect complementary as siRNA. Commercially available miRNA mimics are duplex RNA with the * strand heavily chemically modified to suppress its off-target activity. The socalled miRNA precursor sold by Ambion is a misnomer, it is not pre-miRNA, but short duplex.