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Digestion Related Discussions
  1. Cleaning up RE digest results - advice wanted (with pictures) - (reply: 2)
  2. Troubleshooting with restriction enzymes during cloning - (reply: 5)
  3. restriction enzyme pcr cloning - (reply: 1)
  4. Restriction enzyme - (reply: 1)
  5. Restriction Digest with pGEMT for TA cloning - (reply: 19)
  6. Need to run gel after miniprep before digest? - (reply: 1)
  7. Use of speedvac to dry in-gel peptide digest before sending for LCMS - (reply: 2)
  8. Problem with restriction digestion of cloned PCR product - (reply: 5)
  9. RNase-A digestion in NEB cutsmart buffer - (reply: 1)
  10. Doing a double digest - (reply: 3)
  11. Problem with double digest cloning - (reply: 3)
  12. Can restriction enzymes cleave RNA? - (reply: 1)
  13. Double Digest pCD Vector - (reply: 3)
  14. Double digestion problem with PUCsp vector - (reply: 1)
  15. RE digestion and ligation troubleshooting. - (reply: 1)
  16. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
  17. Does collagenase digestion require quenching? - (reply: 2)
  18. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
  19. Problem with BamHI Digestion - (reply: 7)
  20. Restriction double digest - (reply: 1)
  21. Digestion bands appear faint - (reply: 3)
  22. trypsin digestion of 2D-PAGE protein spot - (reply: 2)
  23. Restriction digestion and CIP - (reply: 2)
  24. Digest two sites located next to each other - (reply: 5)
  25. Does a restriction enzyme cut at a related site? - (reply: 5)
  26. primer design with restriction enzyme - (reply: 1)
  27. ClaI restriction enzyme efficency - (reply: 1)
  28. sequantial digest - (reply: 25)
  29. DMSO in restriction digest - (reply: 1)
  30. Partial digest XhoI NotI NEB - (reply: 6)
  31. Do you recommend Fermentas restriction enzymes? - (reply: 5)
  32. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  33. restriction enzymes/sites to avoid - (reply: 4)
  34. Digestion necessary after PCR? - (reply: 9)
  35. Problem in plasmid digestion after transformation - (reply: 4)
  36. Low RNA quantity after on column DNAse digestion - (reply: 1)
  37. Small yield of RNA after on column DNAs digestion , Why - (reply: 3)
  38. Glycogen affect restriction enzyme digestion? - (reply: 1)
  39. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
  40. Loss of DNA after restriction digestion cleanup - (reply: 2)
  41. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  42. Restriction Digest Question - (reply: 4)
  43. Plasmid DNA digestion problem - (reply: 3)
  44. Plasmid digestion and gel purification using HindIII - (reply: 3)
  45. New ordered Restriction Enzymes not working, need help! - (reply: 2)
  46. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  47. RNase digestion followed by Radiolabelling iCLIP/CLIP - (reply: 1)
  48. Colony PCR positive and Digestion negative????? - (reply: 11)
  49. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  50. different restriction enzymes different companies, can it work?? - (reply: 1)
  51. Digestion using Collagenase and dispase - (reply: 1)
  52. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
  53. Question about DNA purification from a restriction digestion reaction - (reply: 1)
  54. Question about Double Digestion followed by PCR amplification - (reply: 2)
  55. Column for removing short DNA digestion products - (reply: 6)
  56. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
  57. Double digestion with BamHI and BglII - (reply: 4)
  58. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
  59. Problems with Digestion? - (reply: 7)
  60. plasmid digestion - problem with enzymes? - (reply: 4)
  61. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
  62. Sequencing problem and restricition enzyme digestion - (reply: 9)
  63. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  64. Do I need to sequence after digestion? - (reply: 9)
  65. Problem with double digestion: one enzyme is not working - (reply: 8)
  66. Deleting restriction enzyme sites - (reply: 1)
  67. Restriction digest band shift? - (reply: 1)
  68. In solution digestion - (reply: 2)
  69. PCR and restriction enzyme digestion - (reply: 3)
  70. Probelm with double digestion - (reply: 1)
  71. How to set up simultaneous digestion? - (reply: 3)
  72. restriction enzyme - (reply: 2)
  73. SalI and double digestion. - (reply: 2)
  74. Restriction Enzyme Digest not working - (reply: 2)
  75. Incomplete digestion of plasmid with single enzyme - (reply: 1)
  76. problem with plasmid digestion - (reply: 5)
  77. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
  78. can restriction enzymes be stored at -70°C? - (reply: 3)
  79. Restriction Enzymes - (reply: 4)
  80. cloning: band at different site than desired after RE digestion - (reply: 11)
  81. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
  82. pcr product digestion problem - (reply: 4)
  83. Digest does not confirm presence of fragment - (reply: 5)
  84. Does Proteinase K digest RNAse in lysis buffer? - (reply: 2)
  85. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
  86. Restriction enzymes for a gene - (reply: 2)
  87. Proteinase K digestion time. - (reply: 5)
  88. no product on gel after restriction digestion - (reply: 3)
  89. Plasmid Purification and Digestion Problem - (reply: 2)
  90. mass spectrometry and tryptic digestion on membrane proteins - (reply: 5)
  91. Vector Digestion Problem - (reply: 4)
  92. Colony PCR and digestion with KpnI - (reply: 10)
  93. Subcloning - RE digest and ligation and transformation - (reply: 5)
  94. Clarification on digesting a ligation mixture! - (reply: 1)
  95. Use PCR purification kit to purify restriction digestion - (reply: 2)
  96. No bands at all after double digestion - (reply: 1)
  97. Digest for insert orientation - (reply: 4)
  98. digestion and labelling of large ammount of DNA - (reply: 2)
  99. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
  100. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
  101. problem of restriction enzyme digestion - (reply: 3)
  102. Simoltanious Multi-restriction digestion - (reply: 3)
  103. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
  104. Restriction digestion problems - (reply: 3)
  105. Problem in Vector-Insert digestion result - (reply: 1)
  106. Digest with AarI and Pml I - (reply: 1)
  107. SDS-PAGE staining for digestion assay - (reply: 1)
  108. Problems with Restriction Digest Results - (reply: 7)
  109. Digestion - (reply: 2)
  110. Digestion of Genomic DNA - (reply: 1)
  111. Need help troubleshooting digest. - (reply: 1)
  112. Help with unknown band in restriction digest - (reply: 1)
  113. Sequential Restriction Enzyme Digestion - (reply: 1)
  114. problem in cloning involving partial digestion - (reply: 1)
  115. Why perform restriction enzyme digestion again? - (reply: 1)
  116. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
  117. Plasmid digestion after transformation - (reply: 4)
  118. Puzzle about plamid and its digest, plz help - (reply: 8)
  119. restriction digestion buffers contaminated with dnase - (reply: 3)
  120. smear restriction digest - (reply: 1)
  121. How to Prepare Digestion Buffer - (reply: 4)
  122. troubles in DNA digestion - (reply: 7)
  123. Enzymetic digestion with ApaI and HincII - (reply: 2)
  124. I've run out of ideas.... Restriction enzyme digestion - (reply: 6)
  125. double digest problem - (reply: 3)
  126. Restriction Digest Problem - (reply: 5)
  127. Double digest after cloning doesn't give gene - (reply: 6)
  128. Problem with restriction digestion of agarose-embedded DNA - (reply: 4)
  129. Help in RE digestion of vector - (reply: 5)
  130. How to add restriction enzyme site into the DNA fragment - (reply: 3)
  131. Plasmid digestion create a bigger plasmid - (reply: 1)
  132. Determining optimal digestion time for partial digestion - (reply: 2)
  133. double digestion with sac1, hindIII - (reply: 3)
  134. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
  135. help in digesting DNA fragment using restriction enzyme - (reply: 2)
  136. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
  137. Restriction digestion of clones give undesired product - (reply: 3)
  138. XhoI digestion - (reply: 12)
  139. Distance between RE sites for double digestion - (reply: 5)
  140. Thrombin removel His tag protein digestion - (reply: 1)
  141. Random digest of double stranded DNA - (reply: 1)
  142. real-time PCR after restriction enzyme digestion - (reply: 6)
  143. how to purify Fc fragment - Fc fragment purification after papain digestion (reply: 8)
  144. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
  145. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
  146. Double digestion with NcoI and XhoI - (reply: 3)
  147. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
  148. Restriction Enzyme sites in my genes - (reply: 1)
  149. 126pb insert clonnig/ digestion issues - (reply: 3)
  150. problem in digestion - (reply: 1)
  151. restriction digestion against colony PCR - (reply: 1)
  152. how long for a double digestion - (reply: 2)
  153. RE digestion - (reply: 4)
  154. No bands on agarose gel after restriction digestion. - (reply: 10)
  155. metylated cloning vector - restriction digest of the insert (reply: 2)
  156. Frequency of restriction enzyme sites in a genome - (reply: 5)
  157. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
  158. Digestion of mini-prep by mistake - (reply: 3)
  159. restriction digestion - (reply: 1)
  160. digestion with BamHI - SacI - molecular biology (reply: 5)
  161. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
  162. restriction digest simple question - (reply: 4)
  163. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  164. bizarre digestion:) - (reply: 2)
  165. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
  166. Restriction Digestion!! - (reply: 5)
  167. restriction digest - smear - (reply: 1)
  168. Adding restriction enzyme sites to sequences - (reply: 7)
  169. Restriction Digest Assignment Difficulty - (reply: 3)
  170. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
  171. PAS negative - amylase or diastase digestion (reply: 1)
  172. gDNA restriction digest - (reply: 1)
  173. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  174. Digestion problem XbaI, methylation sensitive site - (reply: 3)
  175. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
  176. Digesting large amount of plasmid - (reply: 4)
  177. cleanup of digestion before ligation - (reply: 6)
  178. How to extract restriction enzyme (or restriction endonuclease) from bacteria? - (reply: 1)
  179. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
  180. Restriction digestion of gDNA for phage library - (reply: 5)
  181. No band after digestion - (reply: 4)
  182. Restriction enzyme digestion problem - (reply: 2)
  183. restriction digestion - (reply: 7)
  184. Slight Smear after Digestion - (reply: 2)
  185. Can you see any digestion? - (reply: 5)
  186. problem about pIRES2-DsRed2 double digestion - (reply: 2)
  187. digestion of insert twice and ligation of 3 bits - (reply: 6)
  188. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
  189. Restriction Digest Question - regarding in silico resources (reply: 2)
  190. Double digestion and ligation problem - (reply: 1)
  191. restriction digestion - (reply: 7)
  192. Double Digest - (reply: 2)
  193. Self-ligation from a Double Digest - (reply: 4)
  194. BstUI digestion - (reply: 1)
  195. Problem with SmaI restriction digest - (reply: 6)
  196. Double digestion using EcoRI and HindIII with pHAT-CAT - (reply: 4)
  197. What is the minimum DNA concentration requried for double digestion? - (reply: 1)
  198. Streaky DNA digest - (reply: 3)
  199. Nco1 digest problem - (reply: 4)
  200. restriction enzyme query - (reply: 3)
  201. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
  202. Cell Digestion Protocol - (reply: 1)
  203. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
  204. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
  205. How to make digestion buffer? - (reply: 4)
  206. Problem with a Double digestion - ClaI/Esp3I digestion does not want to work! (reply: 6)
  207. restriction enzyme flanking sequence preferance - (reply: 2)
  208. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  209. Double digestion and cloning - (reply: 2)
  210. Restriction digestion - (reply: 6)
  211. Time required for digestion using Fast digest enzymes - (reply: 7)
  212. restriction digestion of PCR product - (reply: 7)
  213. Colony PCR screen positive - insert digestion negative - (reply: 2)
  214. Restriction enzyme buffer running out ? - (reply: 1)
  215. AdEasy Vector System: Why PacI digestion? - (reply: 1)
  216. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
  217. question about restriction enzyme digest - (reply: 1)
  218. how to get sharp band for restriction enzyme - how to get sharp band for restriction enzyme (reply: 2)
  219. smear for digestion - (reply: 11)
  220. Restriction enzymes - (reply: 3)
  221. Need help for restriction enzymes - (reply: 5)
  222. Restriction digest - (reply: 7)
  223. Help! Double restriction digestion failed.... - (reply: 6)
  224. Digestion Protocols - (reply: 1)
  225. HELP! Can't double digest pcDNA3.1 vector - (reply: 6)
  226. RE digest problem - (reply: 6)
  227. The protocol for double digestion with BamHI and SacI - (reply: 15)
  228. Restriction digestion troubleshooting - (reply: 10)
  229. Which digestion enzyme? - (reply: 1)
  230. protein digestion - (reply: 2)
  231. How To Fix NaOH/Tris DNA Digest Error? - (reply: 1)
  232. Brain tumour tissue enzymatic digestion for culturing cells - (reply: 2)
  233. NEB Restriction Enzymes and Buffer - Restriction (reply: 13)
  234. PCR product disappears after restriction digestion - (reply: 4)
  235. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
  236. Chimeric antibody Pepsin digestion - (reply: 1)
  237. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
  238. 5-aza-2'-deoxycytidine inhibits digest? - Can this base analog inhibit RE-digest? (reply: 1)
  239. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
  240. Is gDNA digestion before bisulfite conversion important? - (reply: 5)
  241. Troubleshooting restriction digest - (reply: 1)
  242. Xho1 doesn't digest pEGFP.N2 completely, why? - (reply: 6)
  243. two step digestion - (reply: 6)
  244. BamHI restriction digest - (reply: 9)
  245. restriction enzyme problem - restriction enzyme problem (reply: 4)
  246. No product after restriction digestion - (reply: 26)
  247. Basic doubt on restriction digestion - (reply: 2)
  248. restriction digestion of PURIFIED PRODUCT - (reply: 5)
  249. MBO1 digestion - (reply: 4)
  250. cDNA synthesis - need amplified cDNA to do RE digestion (reply: 6)
  251. strange extra bands on digest of miniprep? - (reply: 5)
  252. need help with agarose gel electrophoresis and restriction enzyme - (reply: 7)
  253. sequential digest trouble with pUC 19 and XbaI - (reply: 1)
  254. Changing Restriction Enzyme Sites with oligos - (reply: 2)
  255. Smear in my restriction digest - (reply: 12)
  256. Abnormal Nanodrop Results for pTurbo EcoRI digest - (reply: 7)
  257. Double Digest using enzymes from diff companies - (reply: 4)
  258. How to clean up restriction enzyme digestion - (reply: 8)
  259. full digestion of plasmid to get only dNTPs - (reply: 3)
  260. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
  261. dpn1 digest - (reply: 5)
  262. Dnase digestion and PCR - (reply: 4)
  263. Digestion of a plasmid - (reply: 1)
  264. different digestion pattern for same clone - (reply: 4)
  265. T4 DNA Ligase & Restriction Enzyme Expression - I am looking to save money! (reply: 4)
  266. RNA appearance on 1.5% gel after DNase I digestion - (reply: 2)
  267. Troubleshooting Digest, ligation, transformation - (reply: 7)
  268. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
  269. Ligation after SalI & XhoI Digestion - (reply: 3)
  270. CIP required for double digestion of plasmid? - (reply: 3)
  271. Chromatin Remodelling / restriction enzyme accessibility - (reply: 1)
  272. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  273. Sequential restriction digestion of plasmid - (reply: 58)
  274. restriction enzyme based on forward or reverse sequence - restriction enzyme based on forward or reverse sequence (reply: 2)
  275. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  276. problem in double digestion - (reply: 2)
  277. How to detect incomplete enzyme digests - (reply: 3)
  278. double digestion not working - (reply: 4)
  279. After double digestion, one weird band on the very top! - (reply: 4)
  280. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)
  281. Restriction enzyme digest - (reply: 19)