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Digestion Related Discussions
  1. Need to run gel after miniprep before digest? - (reply: 1)
  2. Use of speedvac to dry in-gel peptide digest before sending for LCMS - (reply: 2)
  3. Problem with restriction digestion of cloned PCR product - (reply: 5)
  4. RNase-A digestion in NEB cutsmart buffer - (reply: 1)
  5. Doing a double digest - (reply: 3)
  6. Problem with double digest cloning - (reply: 3)
  7. Can restriction enzymes cleave RNA? - (reply: 1)
  8. Double Digest pCD Vector - (reply: 3)
  9. Double digestion problem with PUCsp vector - (reply: 1)
  10. RE digestion and ligation troubleshooting. - (reply: 1)
  11. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
  12. Does collagenase digestion require quenching? - (reply: 2)
  13. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
  14. Problem with BamHI Digestion - (reply: 7)
  15. Restriction double digest - (reply: 1)
  16. Digestion bands appear faint - (reply: 3)
  17. trypsin digestion of 2D-PAGE protein spot - (reply: 2)
  18. Restriction digestion and CIP - (reply: 2)
  19. Digest two sites located next to each other - (reply: 5)
  20. Does a restriction enzyme cut at a related site? - (reply: 5)
  21. primer design with restriction enzyme - (reply: 1)
  22. ClaI restriction enzyme efficency - (reply: 1)
  23. sequantial digest - (reply: 25)
  24. DMSO in restriction digest - (reply: 1)
  25. Partial digest XhoI NotI NEB - (reply: 6)
  26. Do you recommend Fermentas restriction enzymes? - (reply: 5)
  27. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  28. restriction enzymes/sites to avoid - (reply: 4)
  29. Digestion necessary after PCR? - (reply: 9)
  30. Problem in plasmid digestion after transformation - (reply: 4)
  31. Low RNA quantity after on column DNAse digestion - (reply: 1)
  32. Small yield of RNA after on column DNAs digestion , Why - (reply: 3)
  33. Glycogen affect restriction enzyme digestion? - (reply: 1)
  34. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
  35. Loss of DNA after restriction digestion cleanup - (reply: 2)
  36. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  37. Restriction Digest Question - (reply: 4)
  38. Plasmid DNA digestion problem - (reply: 3)
  39. Plasmid digestion and gel purification using HindIII - (reply: 3)
  40. New ordered Restriction Enzymes not working, need help! - (reply: 2)
  41. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  42. RNase digestion followed by Radiolabelling iCLIP/CLIP - (reply: 1)
  43. Colony PCR positive and Digestion negative????? - (reply: 11)
  44. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  45. different restriction enzymes different companies, can it work?? - (reply: 1)
  46. Digestion using Collagenase and dispase - (reply: 1)
  47. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
  48. Question about DNA purification from a restriction digestion reaction - (reply: 1)
  49. Question about Double Digestion followed by PCR amplification - (reply: 2)
  50. Column for removing short DNA digestion products - (reply: 6)
  51. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
  52. Double digestion with BamHI and BglII - (reply: 4)
  53. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
  54. Problems with Digestion? - (reply: 7)
  55. plasmid digestion - problem with enzymes? - (reply: 4)
  56. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
  57. Sequencing problem and restricition enzyme digestion - (reply: 9)
  58. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  59. Do I need to sequence after digestion? - (reply: 9)
  60. Problem with double digestion: one enzyme is not working - (reply: 8)
  61. Deleting restriction enzyme sites - (reply: 1)
  62. Restriction digest band shift? - (reply: 1)
  63. In solution digestion - (reply: 2)
  64. PCR and restriction enzyme digestion - (reply: 3)
  65. Probelm with double digestion - (reply: 1)
  66. How to set up simultaneous digestion? - (reply: 3)
  67. restriction enzyme - (reply: 2)
  68. SalI and double digestion. - (reply: 2)
  69. Restriction Enzyme Digest not working - (reply: 2)
  70. Incomplete digestion of plasmid with single enzyme - (reply: 1)
  71. problem with plasmid digestion - (reply: 5)
  72. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
  73. can restriction enzymes be stored at -70C? - (reply: 3)
  74. Restriction Enzymes - (reply: 4)
  75. cloning: band at different site than desired after RE digestion - (reply: 11)
  76. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
  77. pcr product digestion problem - (reply: 4)
  78. Digest does not confirm presence of fragment - (reply: 5)
  79. Does Proteinase K digest RNAse in lysis buffer? - (reply: 2)
  80. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
  81. Restriction enzymes for a gene - (reply: 2)
  82. Proteinase K digestion time. - (reply: 5)
  83. no product on gel after restriction digestion - (reply: 3)
  84. Plasmid Purification and Digestion Problem - (reply: 2)
  85. mass spectrometry and tryptic digestion on membrane proteins - (reply: 5)
  86. Vector Digestion Problem - (reply: 4)
  87. Colony PCR and digestion with KpnI - (reply: 10)
  88. Subcloning - RE digest and ligation and transformation - (reply: 5)
  89. Clarification on digesting a ligation mixture! - (reply: 1)
  90. Use PCR purification kit to purify restriction digestion - (reply: 2)
  91. No bands at all after double digestion - (reply: 1)
  92. Digest for insert orientation - (reply: 4)
  93. digestion and labelling of large ammount of DNA - (reply: 2)
  94. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
  95. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
  96. problem of restriction enzyme digestion - (reply: 3)
  97. Simoltanious Multi-restriction digestion - (reply: 3)
  98. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
  99. Restriction digestion problems - (reply: 3)
  100. Problem in Vector-Insert digestion result - (reply: 1)
  101. Digest with AarI and Pml I - (reply: 1)
  102. SDS-PAGE staining for digestion assay - (reply: 1)
  103. Problems with Restriction Digest Results - (reply: 7)
  104. Digestion - (reply: 2)
  105. Digestion of Genomic DNA - (reply: 1)
  106. Need help troubleshooting digest. - (reply: 1)
  107. Help with unknown band in restriction digest - (reply: 1)
  108. Sequential Restriction Enzyme Digestion - (reply: 1)
  109. problem in cloning involving partial digestion - (reply: 1)
  110. Why perform restriction enzyme digestion again? - (reply: 1)
  111. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
  112. Plasmid digestion after transformation - (reply: 4)
  113. Puzzle about plamid and its digest, plz help - (reply: 8)
  114. restriction digestion buffers contaminated with dnase - (reply: 3)
  115. smear restriction digest - (reply: 1)
  116. How to Prepare Digestion Buffer - (reply: 4)
  117. troubles in DNA digestion - (reply: 7)
  118. Enzymetic digestion with ApaI and HincII - (reply: 2)
  119. I've run out of ideas.... Restriction enzyme digestion - (reply: 6)
  120. double digest problem - (reply: 3)
  121. Restriction Digest Problem - (reply: 5)
  122. Double digest after cloning doesn't give gene - (reply: 6)
  123. Problem with restriction digestion of agarose-embedded DNA - (reply: 4)
  124. Help in RE digestion of vector - (reply: 5)
  125. How to add restriction enzyme site into the DNA fragment - (reply: 3)
  126. Plasmid digestion create a bigger plasmid - (reply: 1)
  127. double digestion with sac1, hindIII - (reply: 3)
  128. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
  129. help in digesting DNA fragment using restriction enzyme - (reply: 2)
  130. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
  131. Restriction digestion of clones give undesired product - (reply: 3)
  132. XhoI digestion - (reply: 12)
  133. Distance between RE sites for double digestion - (reply: 5)
  134. Thrombin removel His tag protein digestion - (reply: 1)
  135. Random digest of double stranded DNA - (reply: 1)
  136. real-time PCR after restriction enzyme digestion - (reply: 6)
  137. how to purify Fc fragment - Fc fragment purification after papain digestion (reply: 8)
  138. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
  139. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
  140. Double digestion with NcoI and XhoI - (reply: 3)
  141. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
  142. Restriction Enzyme sites in my genes - (reply: 1)
  143. 126pb insert clonnig/ digestion issues - (reply: 3)
  144. problem in digestion - (reply: 1)
  145. restriction digestion against colony PCR - (reply: 1)
  146. how long for a double digestion - (reply: 2)
  147. RE digestion - (reply: 4)
  148. No bands on agarose gel after restriction digestion. - (reply: 10)
  149. metylated cloning vector - restriction digest of the insert (reply: 2)
  150. Frequency of restriction enzyme sites in a genome - (reply: 5)
  151. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
  152. Digestion of mini-prep by mistake - (reply: 3)
  153. restriction digestion - (reply: 1)
  154. digestion with BamHI - SacI - molecular biology (reply: 5)
  155. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
  156. restriction digest simple question - (reply: 4)
  157. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  158. bizarre digestion:) - (reply: 2)
  159. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
  160. Restriction Digestion!! - (reply: 5)
  161. restriction digest - smear - (reply: 1)
  162. Adding restriction enzyme sites to sequences - (reply: 7)
  163. Restriction Digest Assignment Difficulty - (reply: 3)
  164. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
  165. PAS negative - amylase or diastase digestion (reply: 1)
  166. gDNA restriction digest - (reply: 1)
  167. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  168. Digestion problem XbaI, methylation sensitive site - (reply: 3)
  169. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
  170. Digesting large amount of plasmid - (reply: 4)
  171. cleanup of digestion before ligation - (reply: 6)
  172. How to extract restriction enzyme (or restriction endonuclease) from bacteria? - (reply: 1)
  173. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
  174. Restriction digestion of gDNA for phage library - (reply: 5)
  175. No band after digestion - (reply: 4)
  176. Restriction enzyme digestion problem - (reply: 2)
  177. restriction digestion - (reply: 7)
  178. Slight Smear after Digestion - (reply: 2)
  179. Can you see any digestion? - (reply: 5)
  180. problem about pIRES2-DsRed2 double digestion - (reply: 2)
  181. digestion of insert twice and ligation of 3 bits - (reply: 6)
  182. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
  183. Restriction Digest Question - regarding in silico resources (reply: 2)
  184. Double digestion and ligation problem - (reply: 1)
  185. restriction digestion - (reply: 7)
  186. Double Digest - (reply: 2)
  187. Self-ligation from a Double Digest - (reply: 4)
  188. BstUI digestion - (reply: 1)
  189. Problem with SmaI restriction digest - (reply: 6)
  190. Double digestion using EcoRI and HindIII with pHAT-CAT - (reply: 4)
  191. What is the minimum DNA concentration requried for double digestion? - (reply: 1)
  192. Streaky DNA digest - (reply: 3)
  193. Nco1 digest problem - (reply: 4)
  194. restriction enzyme query - (reply: 3)
  195. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
  196. Cell Digestion Protocol - (reply: 1)
  197. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
  198. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
  199. How to make digestion buffer? - (reply: 4)
  200. Problem with a Double digestion - ClaI/Esp3I digestion does not want to work! (reply: 6)
  201. restriction enzyme flanking sequence preferance - (reply: 2)
  202. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  203. Double digestion and cloning - (reply: 2)
  204. Restriction digestion - (reply: 6)
  205. Time required for digestion using Fast digest enzymes - (reply: 7)
  206. restriction digestion of PCR product - (reply: 7)
  207. Colony PCR screen positive - insert digestion negative - (reply: 2)
  208. Restriction enzyme buffer running out ? - (reply: 1)
  209. AdEasy Vector System: Why PacI digestion? - (reply: 1)
  210. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
  211. question about restriction enzyme digest - (reply: 1)
  212. how to get sharp band for restriction enzyme - how to get sharp band for restriction enzyme (reply: 2)
  213. smear for digestion - (reply: 11)
  214. Restriction enzymes - (reply: 3)
  215. Need help for restriction enzymes - (reply: 5)
  216. Restriction digest - (reply: 7)
  217. Help! Double restriction digestion failed.... - (reply: 6)
  218. Digestion Protocols - (reply: 1)
  219. HELP! Can't double digest pcDNA3.1 vector - (reply: 6)
  220. RE digest problem - (reply: 6)
  221. The protocol for double digestion with BamHI and SacI - (reply: 15)
  222. Restriction digestion troubleshooting - (reply: 10)
  223. Which digestion enzyme? - (reply: 1)
  224. protein digestion - (reply: 2)
  225. How To Fix NaOH/Tris DNA Digest Error? - (reply: 1)
  226. Brain tumour tissue enzymatic digestion for culturing cells - (reply: 2)
  227. NEB Restriction Enzymes and Buffer - Restriction (reply: 13)
  228. PCR product disappears after restriction digestion - (reply: 4)
  229. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
  230. Chimeric antibody Pepsin digestion - (reply: 1)
  231. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
  232. 5-aza-2'-deoxycytidine inhibits digest? - Can this base analog inhibit RE-digest? (reply: 1)
  233. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
  234. Is gDNA digestion before bisulfite conversion important? - (reply: 5)
  235. Troubleshooting restriction digest - (reply: 1)
  236. Xho1 doesn't digest pEGFP.N2 completely, why? - (reply: 6)
  237. two step digestion - (reply: 6)
  238. BamHI restriction digest - (reply: 9)
  239. restriction enzyme problem - restriction enzyme problem (reply: 4)
  240. No product after restriction digestion - (reply: 26)
  241. Basic doubt on restriction digestion - (reply: 2)
  242. restriction digestion of PURIFIED PRODUCT - (reply: 5)
  243. MBO1 digestion - (reply: 4)
  244. cDNA synthesis - need amplified cDNA to do RE digestion (reply: 6)
  245. strange extra bands on digest of miniprep? - (reply: 5)
  246. need help with agarose gel electrophoresis and restriction enzyme - (reply: 7)
  247. sequential digest trouble with pUC 19 and XbaI - (reply: 1)
  248. Changing Restriction Enzyme Sites with oligos - (reply: 2)
  249. Smear in my restriction digest - (reply: 12)
  250. Abnormal Nanodrop Results for pTurbo EcoRI digest - (reply: 7)
  251. Double Digest using enzymes from diff companies - (reply: 4)
  252. How to clean up restriction enzyme digestion - (reply: 8)
  253. full digestion of plasmid to get only dNTPs - (reply: 3)
  254. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
  255. dpn1 digest - (reply: 5)
  256. Dnase digestion and PCR - (reply: 4)
  257. Digestion of a plasmid - (reply: 1)
  258. different digestion pattern for same clone - (reply: 4)
  259. T4 DNA Ligase & Restriction Enzyme Expression - I am looking to save money! (reply: 4)
  260. RNA appearance on 1.5% gel after DNase I digestion - (reply: 2)
  261. Troubleshooting Digest, ligation, transformation - (reply: 7)
  262. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
  263. Ligation after SalI & XhoI Digestion - (reply: 3)
  264. CIP required for double digestion of plasmid? - (reply: 3)
  265. Chromatin Remodelling / restriction enzyme accessibility - (reply: 1)
  266. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  267. Sequential restriction digestion of plasmid - (reply: 58)
  268. restriction enzyme based on forward or reverse sequence - restriction enzyme based on forward or reverse sequence (reply: 2)
  269. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  270. problem in double digestion - (reply: 2)
  271. How to detect incomplete enzyme digests - (reply: 3)
  272. double digestion not working - (reply: 4)
  273. After double digestion, one weird band on the very top! - (reply: 4)
  274. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)
  275. Restriction enzyme digest - (reply: 19)