Transformation-Min plasmid reqd - (Mar/26/2007 )
I will receive one plasmid from other lab and I just got an email gtom them that they are sending only 5µl and he concentration is about 100ng/µl. Don't ask me why they sent only 5µl but now I will have to transform it into DH5a bacteria.
I would like to know how much minimum plasmid amount I should use for this transformation. I usually use 50 or 100µl aliquot of competent DH5a cells.
Many thanks for your suggestions.
(P.S. - I don't want to use all as if something goes wrong, I will be in trouble and we will receive this plasmid from the other lab after a lot of hassle)
Hello Jiang M!
Usually in our lab we transform 50-100 ng of plasmid into a 50µl aliquot of DH5-alpha.
And after all you only need one conlony to prepare a new culture... So don't worry - I guess 1µl might be enough.
You could use 100 pico grams to transform and get a lot of colonies. I use something around 1ng to retransform.
What they are sending is more than sufficient.
Thank you both of you for quick reply. I am very relieved.