Protocol Online logo
Top : Forum Archives: : Molecular Cloning

E.coli promoterless lacZ vector - (Sep/19/2007 )

Hi all,

I need to do some reporter assays in E.coli - something I've not done before. Can anyone recommend a good promoterless lacZ vector to use? I need to co-transfrom another plasmid into the e.coli host too - thsi plasmid will be pACYC184 and is compatible with pMB1 and colE1 derived plasmids so, my reporter plasmid need to be pMB1 or colE1 derived.

Any suggestions would be great!

thanks v much


How about pUC18?

-bitesizebio guy-

Thanks. Will give it a try