Weak affinity chromatography - (Apr/20/2012 )
I am looking to enrich serum IgM for specific antigen utilizing
weak can mean "less specific"; you may use hydrophobic interaction chromatography where you can gear binding by salt concentration or pH...
if you can find the same packing phase as the hplc method then you can run fplc or/and conventional lc. fplc resolution should be similar, conventional may exhibit more band spreading due to slower elution.