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Enzyme Related Discussions
  1. Determining Cofactor for Inactive Enzyme - (reply: 6)
  2. What is the minimum integer n to multiply the Michaelis constant of an enzyme to - (reply: 5)
  3. Reduce protein concentration without reduced enzyme activity - (reply: 3)
  4. Comparing mutant and wild type enzyme kinetics - (reply: 3)
  5. General questions for enzyme assay - (reply: 2)
  6. Can restriction enzymes cleave RNA? - (reply: 1)
  7. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
  8. Effect of reaction volume on enzyme activity? - (reply: 3)
  9. Calculating specific enzyme activity - (reply: 12)
  10. fungal enzymes - (reply: 1)
  11. Pectinase Enzyme Assay - (reply: 1)
  12. Bisubstrate enzyme reaction kinetics - (reply: 1)
  13. Does a restriction enzyme cut at a related site? - (reply: 5)
  14. primer design with restriction enzyme - (reply: 1)
  15. Increase in enzyme activity - (reply: 1)
  16. Enzyme Mechanism Problems - (reply: 6)
  17. ClaI restriction enzyme efficency - (reply: 1)
  18. Enzymes in clinical diagnosis - (reply: 1)
  19. Do you recommend Fermentas restriction enzymes? - (reply: 5)
  20. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  21. Problem with calculations for enzyme activity - (reply: 2)
  22. restriction enzymes/sites to avoid - (reply: 4)
  23. UGT enzyme - (reply: 1)
  24. conversion factors for enzyme kinetics - (reply: 2)
  25. Reselling a supermix/enzyme in a commercial diagnostic kit - (reply: 2)
  26. Glycogen affect restriction enzyme digestion? - (reply: 1)
  27. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
  28. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
  29. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  30. Help on Enzyme Catalysts - (reply: 1)
  31. enzyme problem - (reply: 2)
  32. New ordered Restriction Enzymes not working, need help! - (reply: 2)
  33. Enzyme Kinetics problem - (reply: 1)
  34. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  35. What unit of enzyme amount is Vmax standardized to? - (reply: 3)
  36. different restriction enzymes different companies, can it work?? - (reply: 1)
  37. Nature has engineered the structures of control enzymes so that their activities - (reply: 1)
  38. Enzyme activity Unit conversion - (reply: 1)
  39. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
  40. wise to do an enzyme kinetic assay with unpurified enzyme? - (reply: 11)
  41. Enzymes and buffers from different companies - (reply: 6)
  42. plasmid digestion - problem with enzymes? - (reply: 4)
  43. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
  44. Protein structure modeling for enzymes with point mutations - (reply: 3)
  45. Sequencing problem and restricition enzyme digestion - (reply: 9)
  46. Problem with double digestion: one enzyme is not working - (reply: 8)
  47. Deleting restriction enzyme sites - (reply: 1)
  48. rsaI enzyme - (reply: 1)
  49. Enzyme cutting: low efficiency - (reply: 5)
  50. How to improve enzyme activity? - (reply: 2)
  51. ligation with one ristrection site/enzyme - (reply: 3)
  52. PCR amplification with high fidelity enzyme - (reply: 1)
  53. PCR and restriction enzyme digestion - (reply: 3)
  54. restriction enzyme - (reply: 2)
  55. Restriction Enzyme Digest not working - (reply: 2)
  56. Trouble shoot with radioactive Enzyme activity assay - (reply: 6)
  57. problem with Enzyme purification - (reply: 4)
  58. Incomplete digestion of plasmid with single enzyme - (reply: 1)
  59. can restriction enzymes be stored at -70C? - (reply: 3)
  60. Restriction Enzymes - (reply: 4)
  61. Enzyme assay - (reply: 3)
  62. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
  63. Calculating enzyme activity - (reply: 2)
  64. Characterization of enzyme in crude extract - (reply: 1)
  65. cellulase enzyme - (reply: 2)
  66. initial velocity in an enzyme reaction - (reply: 10)
  67. Enzyme Kinetics - (reply: 2)
  68. Restriction enzymes for a gene - (reply: 2)
  69. Enzyme dependent to temperature - (reply: 4)
  70. unknown enzyme concentration - (reply: 2)
  71. Relationship between total protein and enzyme activity - (reply: 3)
  72. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
  73. Conversion of Absorbance into enzyme units - (reply: 6)
  74. How to purify antioxidants enzymes from plant tissue. - (reply: 1)
  75. Enzyme Units RNaseA - (reply: 2)
  76. problem of restriction enzyme digestion - (reply: 3)
  77. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
  78. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
  79. ATPase enzyme assay !!!!! - (reply: 3)
  80. FLASH FREEZING AND ENZYME WORK INQUIRY - (reply: 1)
  81. enzyme activity at 100C - (reply: 5)
  82. Sequential Restriction Enzyme Digestion - (reply: 1)
  83. what is the best way to increase my purified enzyme's concentration??? - (reply: 1)
  84. enzyme related question? - (reply: 4)
  85. Why perform restriction enzyme digestion again? - (reply: 1)
  86. how to make a recombinant enzyme refolded - (reply: 2)
  87. Real Time PCR polymerase enzyme - (reply: 3)
  88. Working principle of enzymes - (reply: 5)
  89. Methylation sensitive enzymes - (reply: 1)
  90. I've run out of ideas.... Restriction enzyme digestion - (reply: 6)
  91. Enzyme estimation - (reply: 1)
  92. How to add restriction enzyme site into the DNA fragment - (reply: 3)
  93. Loss of glucose during enzyme assay - (reply: 2)
  94. Enzyme: Taq stability - (reply: 5)
  95. crude enzyme precipitation is inclusion body? - (reply: 2)
  96. How do I denature Herculase II enzyme? (Alternatives welcome) - (reply: 4)
  97. How is the cell protected from the hydrolase enzymes of the lysosomes? - (reply: 1)
  98. restricted DNA from the enzymes Bam H1, Hind111, Eco R1 and Sau 3A fall in? - (reply: 1)
  99. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
  100. help in digesting DNA fragment using restriction enzyme - (reply: 2)
  101. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
  102. Enzyme restriction - (reply: 8)
  103. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
  104. real-time PCR after restriction enzyme digestion - (reply: 6)
  105. Restriction Enzyme sites in my genes - (reply: 1)
  106. enzymes inactivation - (reply: 3)
  107. cheap and fast enzyme activity assay? - (reply: 1)
  108. Opinions wanted - What is needed to design enzymes capable of the rate enhanceme - (reply: 4)
  109. Beta Glucosidase/Galactosidase enzyme assays issues - The enzyme assays are not working for some reason (reply: 10)
  110. What did Linus Pauling say about enzyme catalysis? - Enzyme dynamics and catalysis (reply: 1)
  111. Carbon fixing enzymes - (reply: 1)
  112. Enzymes Technology - Enzymic Hydrolysis (reply: 1)
  113. Frequency of restriction enzyme sites in a genome - (reply: 5)
  114. Taq DNA polymerase - how can microbes affoard an error-making enzyme? (reply: 2)
  115. inactive enzyme from purification - (reply: 1)
  116. digestion - restriction enzymes (reply: 2)
  117. Adding restriction enzyme sites to sequences - (reply: 7)
  118. Molecular Cell Biology - Trafficking and function of a membrane enzyme (reply: 1)
  119. Calculation of enzyme activity - (reply: 1)
  120. enzyme activity in inhibition assay - (reply: 2)
  121. Specific activity - what does it mean by an enzyme? (reply: 3)
  122. Km values in enzymology - (reply: 1)
  123. Enzyme activity assay - (reply: 1)
  124. Enzyme concentration in mol/mg - (reply: 3)
  125. PCR didn't work - suspect the enzyme (reply: 2)
  126. how to extract enzyme allinase from garlic - (reply: 1)
  127. How to extract restriction enzyme (or restriction endonuclease) from bacteria? - (reply: 1)
  128. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
  129. Restriction enzyme digestion problem - (reply: 2)
  130. never get bands by pfu related enzymes - (reply: 2)
  131. Enzyme to Histology contrast in vessel culture - (reply: 4)
  132. Enzymes for COBRA - (reply: 2)
  133. Taq enzyme works but other enzyme didn't work - (reply: 5)
  134. No enzyme Activity (dioxygenase assay) - (reply: 3)
  135. restriction enzyme query - (reply: 3)
  136. Method to measure enzyme activity - GSS (reply: 2)
  137. Determining how much enzyme to use based on activity units - (reply: 3)
  138. Program for determination of kinetic parameters of an enzyme - (reply: 2)
  139. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
  140. restriction enzyme flanking sequence preferance - (reply: 2)
  141. enzyme assay optimization - (reply: 7)
  142. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
  143. Polymerases voor bisulfite sequencing - any fancy new enzymes? (reply: 4)
  144. Time required for digestion using Fast digest enzymes - (reply: 7)
  145. Is an enzyme protein of 1100 aa too big to be expressed in E coli - (reply: 4)
  146. Restriction enzyme buffer running out ? - (reply: 1)
  147. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
  148. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
  149. question about restriction enzyme digest - (reply: 1)
  150. how to get sharp band for restriction enzyme - how to get sharp band for restriction enzyme (reply: 2)
  151. Restriction enzymes - (reply: 3)
  152. PCR + phusion enzyme = massive errors - (reply: 4)
  153. Need help for restriction enzymes - (reply: 5)
  154. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
  155. SDS-page and enzyme activity - (reply: 1)
  156. Lyophilized enzyme - (reply: 3)
  157. reconstitution of lyophilized enzyme (soybean 15-lipoxygenase-1) - (reply: 2)
  158. Dpn1 enzyme - (reply: 2)
  159. Which digestion enzyme? - (reply: 1)
  160. NEB Restriction Enzymes and Buffer - Restriction (reply: 13)
  161. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
  162. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
  163. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
  164. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
  165. detach RAW264.7 - Who knows an enzyme to detach RAW264.7??? (reply: 3)
  166. is it useful to grow two fungal cultures together for production of enzyme? - (reply: 7)
  167. restriction enzyme problem - restriction enzyme problem (reply: 4)
  168. need help with agarose gel electrophoresis and restriction enzyme - (reply: 7)
  169. Changing Restriction Enzyme Sites with oligos - (reply: 2)
  170. Double Digest using enzymes from diff companies - (reply: 4)
  171. S2251 plasminogen measurement - high values obtained even when enzyme absent? (reply: 1)
  172. How to clean up restriction enzyme digestion - (reply: 8)
  173. Why is km important in mutant enzymes? - (reply: 3)
  174. DNA digestion - Restriction Enzymes (reply: 3)
  175. T4 DNA Ligase & Restriction Enzyme Expression - I am looking to save money! (reply: 4)
  176. Quick ? about Enzyme Buffers - (reply: 3)
  177. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
  178. Chromatin Remodelling / restriction enzyme accessibility - (reply: 1)
  179. Lysosomes - Lysosomal enzymes (reply: 3)
  180. Why is Cleavage of DNA using restrcition enzyme more suitable for genetic engine - !! confused confused confused !! (reply: 4)
  181. Enzyme kinetics - quantification of reversible enzyme inhibition (reply: 1)
  182. lysis buffer to use for enzyme assay - (reply: 1)
  183. Enzyme IU - (reply: 1)
  184. restriction enzyme based on forward or reverse sequence - restriction enzyme based on forward or reverse sequence (reply: 2)
  185. Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
  186. How to detect incomplete enzyme digests - (reply: 3)
  187. Restriction enzyme digest - (reply: 19)