Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Enzyme Related Discussions
  1. FLASH FREEZING AND ENZYME WORK INQUIRY - (reply: 1)
  2. enzyme activity at 100C - (reply: 5)
  3. Sequential Restriction Enzyme Digestion - (reply: 1)
  4. what is the best way to increase my purified enzyme's concentration??? - (reply: 1)
  5. enzyme related question? - (reply: 2)
  6. Why perform restriction enzyme digestion again? - (reply: 1)
  7. how to make a recombinant enzyme refolded - (reply: 2)
  8. Real Time PCR polymerase enzyme - (reply: 3)
  9. Working principle of enzymes - (reply: 5)
  10. Methylation sensitive enzymes - (reply: 1)
  11. I've run out of ideas.... Restriction enzyme digestion - (reply: 6)
  12. Enzyme estimation - (reply: 1)
  13. How to add restriction enzyme site into the DNA fragment - (reply: 3)
  14. Loss of glucose during enzyme assay - (reply: 2)
  15. Enzyme: Taq stability - (reply: 5)
  16. crude enzyme precipitation is inclusion body? - (reply: 2)
  17. How do I denature Herculase II enzyme? (Alternatives welcome) - (reply: 4)
  18. How is the cell protected from the hydrolase enzymes of the lysosomes? - (reply: 1)
  19. restricted DNA from the enzymes Bam H1, Hind111, Eco R1 and Sau 3A fall in? - (reply: 1)
  20. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
  21. help in digesting DNA fragment using restriction enzyme - (reply: 2)
  22. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
  23. Enzyme restriction - (reply: 8)
  24. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
  25. real-time PCR after restriction enzyme digestion - (reply: 6)
  26. Restriction Enzyme sites in my genes - (reply: 1)
  27. enzymes inactivation - (reply: 3)
  28. cheap and fast enzyme activity assay? - (reply: 1)
  29. Opinions wanted - What is needed to design enzymes capable of the rate enhanceme - (reply: 4)
  30. Beta Glucosidase/Galactosidase enzyme assays issues - The enzyme assays are not working for some reason (reply: 10)
  31. What did Linus Pauling say about enzyme catalysis? - Enzyme dynamics and catalysis (reply: 1)
  32. Carbon fixing enzymes - (reply: 1)
  33. Enzymes Technology - Enzymic Hydrolysis (reply: 1)
  34. Frequency of restriction enzyme sites in a genome - (reply: 5)
  35. Taq DNA polymerase - how can microbes affoard an error-making enzyme? (reply: 2)
  36. inactive enzyme from purification - (reply: 1)
  37. digestion - restriction enzymes (reply: 2)
  38. Adding restriction enzyme sites to sequences - (reply: 7)
  39. Molecular Cell Biology - Trafficking and function of a membrane enzyme (reply: 1)
  40. Calculation of enzyme activity - (reply: 1)
  41. enzyme activity in inhibition assay - (reply: 2)
  42. Specific activity - what does it mean by an enzyme? (reply: 3)
  43. Km values in enzymology - (reply: 1)
  44. Enzyme activity assay - (reply: 1)
  45. Enzyme concentration in mol/mg - (reply: 3)
  46. PCR didn't work - suspect the enzyme (reply: 2)
  47. how to extract enzyme allinase from garlic - (reply: 1)
  48. How to extract restriction enzyme (or restriction endonuclease) from bacteria? - (reply: 1)
  49. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
  50. Restriction enzyme digestion problem - (reply: 2)
  51. never get bands by pfu related enzymes - (reply: 2)
  52. Enzyme to Histology contrast in vessel culture - (reply: 4)
  53. Enzymes for COBRA - (reply: 2)
  54. Taq enzyme works but other enzyme didn't work - (reply: 5)
  55. No enzyme Activity (dioxygenase assay) - (reply: 3)
  56. restriction enzyme query - (reply: 3)
  57. Method to measure enzyme activity - GSS (reply: 2)
  58. Determining how much enzyme to use based on activity units - (reply: 3)
  59. Program for determination of kinetic parameters of an enzyme - (reply: 2)
  60. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
  61. restriction enzyme flanking sequence preferance - (reply: 2)
  62. enzyme assay optimization - (reply: 7)
  63. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
  64. Polymerases voor bisulfite sequencing - any fancy new enzymes? (reply: 4)
  65. Time required for digestion using Fast digest enzymes - (reply: 7)
  66. Is an enzyme protein of 1100 aa too big to be expressed in E coli - (reply: 4)
  67. Restriction enzyme buffer running out ? - (reply: 1)
  68. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
  69. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
  70. question about restriction enzyme digest - (reply: 1)
  71. how to get sharp band for restriction enzyme - how to get sharp band for restriction enzyme (reply: 2)
  72. Restriction enzymes - (reply: 3)
  73. PCR + phusion enzyme = massive errors - (reply: 4)
  74. Need help for restriction enzymes - (reply: 5)
  75. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
  76. SDS-page and enzyme activity - (reply: 1)
  77. Lyophilized enzyme - (reply: 3)
  78. reconstitution of lyophilized enzyme (soybean 15-lipoxygenase-1) - (reply: 2)
  79. Dpn1 enzyme - (reply: 2)
  80. Which digestion enzyme? - (reply: 1)
  81. NEB Restriction Enzymes and Buffer - Restriction (reply: 13)
  82. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
  83. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
  84. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
  85. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
  86. detach RAW264.7 - Who knows an enzyme to detach RAW264.7??? (reply: 3)
  87. is it useful to grow two fungal cultures together for production of enzyme? - (reply: 7)
  88. restriction enzyme problem - restriction enzyme problem (reply: 4)
  89. need help with agarose gel electrophoresis and restriction enzyme - (reply: 7)
  90. Changing Restriction Enzyme Sites with oligos - (reply: 2)
  91. Double Digest using enzymes from diff companies - (reply: 4)
  92. S2251 plasminogen measurement - high values obtained even when enzyme absent? (reply: 1)
  93. How to clean up restriction enzyme digestion - (reply: 8)
  94. Why is km important in mutant enzymes? - (reply: 3)
  95. DNA digestion - Restriction Enzymes (reply: 3)
  96. T4 DNA Ligase & Restriction Enzyme Expression - I am looking to save money! (reply: 4)
  97. Quick ? about Enzyme Buffers - (reply: 3)
  98. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
  99. Chromatin Remodelling / restriction enzyme accessibility - (reply: 1)
  100. Lysosomes - Lysosomal enzymes (reply: 3)
  101. Why is Cleavage of DNA using restrcition enzyme more suitable for genetic engine - !! confused confused confused !! (reply: 4)
  102. Enzyme kinetics - quantification of reversible enzyme inhibition (reply: 1)
  103. lysis buffer to use for enzyme assay - (reply: 1)
  104. Enzyme IU - (reply: 1)
  105. restriction enzyme based on forward or reverse sequence - restriction enzyme based on forward or reverse sequence (reply: 2)
  106. Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
  107. How to detect incomplete enzyme digests - (reply: 3)
  108. Restriction enzyme digest - (reply: 19)