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Enzyme Related Discussions
  1. Troubleshooting with restriction enzymes during cloning - (reply: 4)
  2. restriction enzyme pcr cloning - (reply: 1)
  3. How to calculate Vmax and Km from substrate concentration (enzyme reaction)? - (reply: 3)
  4. Restriction enzyme - (reply: 1)
  5. my enzyme shows suicide inhibition, how do i identify the inhibitor? - (reply: 3)
  6. Enzyme prediction - (reply: 2)
  7. Enzyme activity - (reply: 1)
  8. Total recovery of lyophilized enzyme standard ;) - (reply: 1)
  9. Why would enzyme rate of reaction decrease with increasing substrate concentrati - (reply: 4)
  10. Determining Cofactor for Inactive Enzyme - (reply: 6)
  11. What is the minimum integer n to multiply the Michaelis constant of an enzyme to - (reply: 5)
  12. Reduce protein concentration without reduced enzyme activity - (reply: 3)
  13. Comparing mutant and wild type enzyme kinetics - (reply: 3)
  14. General questions for enzyme assay - (reply: 2)
  15. Can restriction enzymes cleave RNA? - (reply: 1)
  16. Cloning several metabolic enzymes to screen for inhibition, how long? - (reply: 1)
  17. Effect of reaction volume on enzyme activity? - (reply: 3)
  18. Calculating specific enzyme activity - (reply: 12)
  19. fungal enzymes - (reply: 1)
  20. Pectinase Enzyme Assay - (reply: 1)
  21. Bisubstrate enzyme reaction kinetics - (reply: 1)
  22. Does a restriction enzyme cut at a related site? - (reply: 5)
  23. primer design with restriction enzyme - (reply: 1)
  24. Increase in enzyme activity - (reply: 1)
  25. Enzyme Mechanism Problems - (reply: 6)
  26. ClaI restriction enzyme efficency - (reply: 1)
  27. Enzymes in clinical diagnosis - (reply: 1)
  28. Do you recommend Fermentas restriction enzymes? - (reply: 5)
  29. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  30. Problem with calculations for enzyme activity - (reply: 2)
  31. restriction enzymes/sites to avoid - (reply: 4)
  32. UGT enzyme - (reply: 1)
  33. conversion factors for enzyme kinetics - (reply: 2)
  34. Reselling a supermix/enzyme in a commercial diagnostic kit - (reply: 2)
  35. Glycogen affect restriction enzyme digestion? - (reply: 1)
  36. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
  37. Sephacryl S-300 didn't enhance the purity of my enzyme - (reply: 3)
  38. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  39. Help on Enzyme Catalysts - (reply: 1)
  40. enzyme problem - (reply: 2)
  41. New ordered Restriction Enzymes not working, need help! - (reply: 2)
  42. Enzyme Kinetics problem - (reply: 1)
  43. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  44. What unit of enzyme amount is Vmax standardized to? - (reply: 3)
  45. different restriction enzymes different companies, can it work?? - (reply: 1)
  46. Nature has engineered the structures of control enzymes so that their activities - (reply: 1)
  47. Enzyme activity Unit conversion - (reply: 1)
  48. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
  49. wise to do an enzyme kinetic assay with unpurified enzyme? - (reply: 11)
  50. Enzymes and buffers from different companies - (reply: 6)
  51. plasmid digestion - problem with enzymes? - (reply: 4)
  52. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
  53. Protein structure modeling for enzymes with point mutations - (reply: 3)
  54. Sequencing problem and restricition enzyme digestion - (reply: 9)
  55. Problem with double digestion: one enzyme is not working - (reply: 8)
  56. Deleting restriction enzyme sites - (reply: 1)
  57. rsaI enzyme - (reply: 1)
  58. Enzyme cutting: low efficiency - (reply: 5)
  59. How to improve enzyme activity? - (reply: 2)
  60. ligation with one ristrection site/enzyme - (reply: 3)
  61. PCR amplification with high fidelity enzyme - (reply: 1)
  62. PCR and restriction enzyme digestion - (reply: 3)
  63. restriction enzyme - (reply: 2)
  64. Restriction Enzyme Digest not working - (reply: 2)
  65. Trouble shoot with radioactive Enzyme activity assay - (reply: 6)
  66. problem with Enzyme purification - (reply: 4)
  67. Incomplete digestion of plasmid with single enzyme - (reply: 1)
  68. can restriction enzymes be stored at -70C? - (reply: 3)
  69. Restriction Enzymes - (reply: 4)
  70. Enzyme assay - (reply: 3)
  71. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
  72. Calculating enzyme activity - (reply: 2)
  73. Characterization of enzyme in crude extract - (reply: 1)
  74. cellulase enzyme - (reply: 2)
  75. initial velocity in an enzyme reaction - (reply: 10)
  76. Enzyme Kinetics - (reply: 2)
  77. Restriction enzymes for a gene - (reply: 2)
  78. Enzyme dependent to temperature - (reply: 4)
  79. unknown enzyme concentration - (reply: 2)
  80. Relationship between total protein and enzyme activity - (reply: 3)
  81. Enzyme storage in eppendorphs to use in enzyme assay next day - (reply: 6)
  82. Conversion of Absorbance into enzyme units - (reply: 6)
  83. How to purify antioxidants enzymes from plant tissue. - (reply: 1)
  84. Enzyme Units RNaseA - (reply: 4)
  85. problem of restriction enzyme digestion - (reply: 3)
  86. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
  87. Active enzyme goes on ion exchange column -- dead enzyme comes off. What am I do - (reply: 3)
  88. ATPase enzyme assay !!!!! - (reply: 3)
  89. FLASH FREEZING AND ENZYME WORK INQUIRY - (reply: 1)
  90. enzyme activity at 100C - (reply: 5)
  91. Sequential Restriction Enzyme Digestion - (reply: 1)
  92. what is the best way to increase my purified enzyme's concentration??? - (reply: 1)
  93. enzyme related question? - (reply: 4)
  94. Why perform restriction enzyme digestion again? - (reply: 1)
  95. how to make a recombinant enzyme refolded - (reply: 2)
  96. Real Time PCR polymerase enzyme - (reply: 3)
  97. Working principle of enzymes - (reply: 5)
  98. Methylation sensitive enzymes - (reply: 1)
  99. I've run out of ideas.... Restriction enzyme digestion - (reply: 6)
  100. Enzyme estimation - (reply: 1)
  101. How to add restriction enzyme site into the DNA fragment - (reply: 3)
  102. Loss of glucose during enzyme assay - (reply: 2)
  103. Enzyme: Taq stability - (reply: 5)
  104. crude enzyme precipitation is inclusion body? - (reply: 2)
  105. How do I denature Herculase II enzyme? (Alternatives welcome) - (reply: 4)
  106. How is the cell protected from the hydrolase enzymes of the lysosomes? - (reply: 1)
  107. restricted DNA from the enzymes Bam H1, Hind111, Eco R1 and Sau 3A fall in? - (reply: 1)
  108. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
  109. help in digesting DNA fragment using restriction enzyme - (reply: 2)
  110. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
  111. Enzyme restriction - (reply: 8)
  112. RT-PCR stoped working!!! Same samples, same primers, same enzyme! - (reply: 3)
  113. real-time PCR after restriction enzyme digestion - (reply: 6)
  114. Restriction Enzyme sites in my genes - (reply: 1)
  115. enzymes inactivation - (reply: 3)
  116. cheap and fast enzyme activity assay? - (reply: 1)
  117. Opinions wanted - What is needed to design enzymes capable of the rate enhanceme - (reply: 4)
  118. Beta Glucosidase/Galactosidase enzyme assays issues - The enzyme assays are not working for some reason (reply: 10)
  119. What did Linus Pauling say about enzyme catalysis? - Enzyme dynamics and catalysis (reply: 1)
  120. Carbon fixing enzymes - (reply: 1)
  121. Enzymes Technology - Enzymic Hydrolysis (reply: 1)
  122. Frequency of restriction enzyme sites in a genome - (reply: 5)
  123. Taq DNA polymerase - how can microbes affoard an error-making enzyme? (reply: 2)
  124. inactive enzyme from purification - (reply: 1)
  125. digestion - restriction enzymes (reply: 2)
  126. Adding restriction enzyme sites to sequences - (reply: 7)
  127. Molecular Cell Biology - Trafficking and function of a membrane enzyme (reply: 1)
  128. Calculation of enzyme activity - (reply: 1)
  129. enzyme activity in inhibition assay - (reply: 2)
  130. Specific activity - what does it mean by an enzyme? (reply: 3)
  131. Km values in enzymology - (reply: 1)
  132. Enzyme activity assay - (reply: 1)
  133. Enzyme concentration in mol/mg - (reply: 3)
  134. PCR didn't work - suspect the enzyme (reply: 2)
  135. how to extract enzyme allinase from garlic - (reply: 1)
  136. How to extract restriction enzyme (or restriction endonuclease) from bacteria? - (reply: 1)
  137. Chromosome Specific Enzymes - Mammalian enzymes that cut only one chromosome in genome (reply: 1)
  138. Restriction enzyme digestion problem - (reply: 2)
  139. never get bands by pfu related enzymes - (reply: 2)
  140. Enzyme to Histology contrast in vessel culture - (reply: 4)
  141. Enzymes for COBRA - (reply: 2)
  142. Taq enzyme works but other enzyme didn't work - (reply: 5)
  143. No enzyme Activity (dioxygenase assay) - (reply: 3)
  144. restriction enzyme query - (reply: 3)
  145. Method to measure enzyme activity - GSS (reply: 2)
  146. Determining how much enzyme to use based on activity units - (reply: 3)
  147. Program for determination of kinetic parameters of an enzyme - (reply: 2)
  148. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
  149. restriction enzyme flanking sequence preferance - (reply: 2)
  150. enzyme assay optimization - (reply: 7)
  151. pcr amolification with long pcr enzyme mix for cloning - (reply: 1)
  152. Polymerases voor bisulfite sequencing - any fancy new enzymes? (reply: 4)
  153. Time required for digestion using Fast digest enzymes - (reply: 7)
  154. Is an enzyme protein of 1100 aa too big to be expressed in E coli - (reply: 4)
  155. Restriction enzyme buffer running out ? - (reply: 1)
  156. SacI enzyme trouble - Is this a good enzyme for cloning? (reply: 2)
  157. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
  158. question about restriction enzyme digest - (reply: 1)
  159. how to get sharp band for restriction enzyme - how to get sharp band for restriction enzyme (reply: 2)
  160. Restriction enzymes - (reply: 3)
  161. PCR + phusion enzyme = massive errors - (reply: 4)
  162. Need help for restriction enzymes - (reply: 5)
  163. Heat inactivation/Gel purification or both? - Normally I purify from gel. Should I inactivate the enzymes? (reply: 2)
  164. SDS-page and enzyme activity - (reply: 1)
  165. Lyophilized enzyme - (reply: 3)
  166. reconstitution of lyophilized enzyme (soybean 15-lipoxygenase-1) - (reply: 2)
  167. Dpn1 enzyme - (reply: 2)
  168. Which digestion enzyme? - (reply: 1)
  169. NEB Restriction Enzymes and Buffer - Restriction (reply: 13)
  170. Accidentally deleted enzyme from Vector NTI - how to restore? - (reply: 1)
  171. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
  172. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
  173. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
  174. detach RAW264.7 - Who knows an enzyme to detach RAW264.7??? (reply: 3)
  175. is it useful to grow two fungal cultures together for production of enzyme? - (reply: 7)
  176. restriction enzyme problem - restriction enzyme problem (reply: 4)
  177. need help with agarose gel electrophoresis and restriction enzyme - (reply: 7)
  178. Changing Restriction Enzyme Sites with oligos - (reply: 2)
  179. Double Digest using enzymes from diff companies - (reply: 4)
  180. S2251 plasminogen measurement - high values obtained even when enzyme absent? (reply: 1)
  181. How to clean up restriction enzyme digestion - (reply: 8)
  182. Why is km important in mutant enzymes? - (reply: 3)
  183. DNA digestion - Restriction Enzymes (reply: 3)
  184. T4 DNA Ligase & Restriction Enzyme Expression - I am looking to save money! (reply: 4)
  185. Quick ? about Enzyme Buffers - (reply: 3)
  186. Problems digesting PCR product - troublesome enzymes: NotI PvuI (reply: 2)
  187. Chromatin Remodelling / restriction enzyme accessibility - (reply: 1)
  188. Lysosomes - Lysosomal enzymes (reply: 3)
  189. Why is Cleavage of DNA using restrcition enzyme more suitable for genetic engine - !! confused confused confused !! (reply: 4)
  190. Enzyme kinetics - quantification of reversible enzyme inhibition (reply: 1)
  191. lysis buffer to use for enzyme assay - (reply: 1)
  192. Enzyme IU - (reply: 1)
  193. restriction enzyme based on forward or reverse sequence - restriction enzyme based on forward or reverse sequence (reply: 2)
  194. Has anyone had experience with GM2163 (dam-) cells? - the enzyme BclI is blocked by dam (reply: 1)
  195. How to detect incomplete enzyme digests - (reply: 3)
  196. Restriction enzyme digest - (reply: 19)