Enzyme Units RNaseA - (Jul/25/2012 )
V1 = (0.01 / 70) x 2000 = 0.285ul
This may be completely wrong but I'm not sure how else to work this out.
this is not a c1v1=c2v2 problem. you are not looking for a final concentration. you are looking for an absolute amount.
you have a 20mg/ml solution of rnase and the specific activity is 70 units/mg (1400 units/ml). you want 20 units to put into your dna solution. 20/70=0.2857.
you, therefore, want approximately 0.29mg (20.3 units). 20mg/ml=1mg/50ul. 0.29x50=14.5ul.
you want to add ~14.5ul of your 20mg/ml rnase solution to your dna solution.
or you can calculate this way:
Thanks! That's much easier than I was playing it up in my head I think.