Pectinase Enzyme Assay - (Aug/18/2014 )

I am working on pectinase enzyme assay. I incubated 900 ul of substrate for 10 minutes in the water bath, followed by adding 2ml of DNSA reagent, then 100ul of enzyme extract added finally i read the absorbance @ 540 OD. However the values are high. How can I troubleshoot high enzyme blank values in Pectinase assay ?

you must give more information about your procedure. for instance, the procedure you wrote appears to be the enzyme-substrate reaction, not a blank. you would expect to see a high reading. how do you prepare your blanks? what are the readings?