Problem with calculations for enzyme activity - (May/08/2014 )
I know it sounds trivial but i have some problems with calculations for the experiment that I've performed today in the lab. I was supposed to do the bioassay for the lysozyme, using 3 samples: crude, precipitation, dialysis and of course standards.
Preparation of satndards: 1mg lysozyme/ml of stock with/ spec activity of 47080 U/mg that needed to be diluted 20 times (I've used a total volume of 500ul, so 25ul in 475 ul of potassiumphosphate buffer)
150 ul of potassiumphosphate buffer
150ul of diluted lysozyme stock
then what is enzyme activity in the standard solution (U/ml)?
I keep making some mistakes and i'm pretty sure of it
So your stock solution is at 1 mg/ml and therefore has 47080 U/ml and you want to know the concentration after dilution
47080 (U/ml) * 25 (ul) = 500 (ul) x C2
You can use the same equation to work out your stand 1.
i was using the concentration for it as 1mg/ml.. and then calculating it further to U/ml
I guess some calculations are usually way easier than I'm thinking