Problem with calculations for enzyme activity - (May/08/2014 )
I know it sounds trivial but i have some problems with calculations for the experiment that I've performed today in the lab. I was supposed to do the bioassay for the lysozyme, using 3 samples: crude, precipitation, dialysis and of course standards.
Preparation of satndards: 1mg lysozyme/ml of stock with/ spec activity of 47080 U/mg that needed to be diluted 20 times (I've used a total volume of 500ul, so 25ul in 475 ul of potassiumphosphate buffer)
Stand 1:
150 ul of potassiumphosphate buffer
150ul of diluted lysozyme stock
then what is enzyme activity in the standard solution (U/ml)?
I keep making some mistakes and i'm pretty sure of it
So your stock solution is at 1 mg/ml and therefore has 47080 U/ml and you want to know the concentration after dilution
C1V1=C2V2
47080 (U/ml) * 25 (ul) = 500 (ul) x C2
C2=??? U/ml
You can use the same equation to work out your stand 1.
thanks :)
i was using the concentration for it as 1mg/ml.. and then calculating it further to U/ml
I guess some calculations are usually way easier than I'm thinking