conversion factors for enzyme kinetics - (Mar/02/2014 )
I need to find the concentration of PNP when I only have initial volumes of PNPG and absorbance meaurements. i have not been given a coefficient, and therefore can't use beer-lambert law.
initial vol PNPG (ml) Absorbance measurements (410nm)
All solutions had a final volume of 10ml, and 2ml of which were enzymes.
I have attempted this myself, and in making graphs i find that ive probably made an error. any insight will be well recieved. thanks kacey
would this help: https://www.goldbio.com/pdf/10458-Protocol%201.pdf ?
if PNP is the enzyme (purine nucleoside phosphorylase) what does PNPG mean?