Protocol Online logo
Top : New Forum Archives (2009-): : Protein Expression and Purification

What make voltage not going up for the 3rd step in running IEF? - (Apr/28/2010 )

I run a 11cm strip for IEF according to the recommended running condition. However, it didn't go up to 8000V at the 3rd step. :( What make it happen?
How to solve it?

-kfszelok-

may be cause u had set a current limit which was reached before reaching 8000V!!!??

-Prep!-

Prep! on Apr 28 2010, 03:51 AM said:

may be cause u had set a current limit which was reached before reaching 8000V!!!??




I second that.

-fishdoc-