Protocol Online logo
Top : Forum Archives: : Molecular Cloning

ligation - (Jun/12/2006 )

i want to ligate a dna fragment (289pb) to do a long fragment
I have the fragment with blunt ends and too with cohesive ends (SalI/XhoI)
which volume and unit of T4 dna ligase must i do ?
thanks very much


I do ligation in a total volume of 20ul, but there r people who do it in 10ul.
I use 1ul of T4 ligase (NEB).