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SiRNA Related Discussions
  1. siRNA Agarose gel electrophoresis - Protocol - (reply: 1)
  2. Apoptosis inducing siRNA? - (reply: 1)
  3. MicroRNA nomenclature - (reply: 4)
  4. siRNA in E.Coli - (reply: 2)
  5. siRNA resuspension in RNase-free water - (reply: 1)
  6. microRNA promoter based expression vector? - (reply: 1)
  7. Wound Scratch Assay using siRNA - (reply: 4)
  8. microRNA validation in vivo - (reply: 1)
  9. Recommendation on siRNA transfection reagents - (reply: 2)
  10. How long is the suppressive efficiency by siRNA or shRNA - (reply: 2)
  11. Synthetic microrna for normalization in serum samples - (reply: 1)
  12. DNA and siRNA transfection and cytotoxicity - (reply: 1)
  13. Hiperfect siRNA in HT-29 cells help - (reply: 2)
  14. SF9 transfection with siRNA - what transfection reagent has worked for you? - (reply: 3)
  15. siRNA modifications - (reply: 2)
  16. Protocol for HT29 siRNA transfection using Lipofectamine 2000? - (reply: 3)
  17. validation of microRNA target gene - (reply: 6)
  18. transfecting siRNA thp-1 after differentiated to macrophage - (reply: 2)
  19. Converting shRNA sequence to siRNA - (reply: 1)
  20. target mRNA/protein levels increase with siRNA - (reply: 2)
  21. One siRNA sequence, many cell lines - (reply: 1)
  22. microRNA exiqon individual assays - (reply: 1)
  23. Need an effective siRNA/ shRNA sequence for downregulating caveolin-1 - (reply: 2)
  24. Is it posssible using shRNA vector to generate microRNA? - (reply: 2)
  25. Calculating siRNA Transfection Efficiency - (reply: 3)
  26. microRNA and 3' UTR - (reply: 2)
  27. siRNA time - (reply: 1)
  28. What to do Post-transfection of cells with siRNA? - (reply: 2)
  29. Which company to buy siRNA from? - (reply: 1)
  30. placing siRNA seq into miRNA stem-loop - (reply: 4)
  31. Help finding siRNA targets in MEF and HEK293 cells needed - (reply: 4)
  32. problem with siRNA transfection of HEK cells - (reply: 5)
  33. Decreased siRNA performance at high concentrations: feasible? - (reply: 1)
  34. Promoter activity of microRNA - (reply: 3)
  35. Phenotypes with two different siRNA but no knockdown? - trouble in showing a knock-down (reply: 2)
  36. siRNA transfection advise - Need some help on siRNA knockdowns (reply: 1)
  37. Help on mIcroRNA expression levels - concentration needed (reply: 1)
  38. siRNA optimization - (reply: 5)
  39. siRNA transfection with lipofectamine 2000 - (reply: 1)
  40. Problems with Scrambled Control - Scrambled controls are inhibiting cell growth more than test siRNA (reply: 2)
  41. siRNA screening and RNAse free - (reply: 1)
  42. miRNA/siRNA transfection doubts - (reply: 2)
  43. PCR of microRNA first strand cDNA - (reply: 1)
  44. How to express a rare microRNA - (reply: 2)
  45. siRNA transfection - 7-day study - serum free media? (reply: 2)
  46. siRNA shearing during light sonication? - (reply: 1)
  47. diffrence between miRNA family and miRNA cluster - diefinitions of microRNA cluster and microRNA family (reply: 1)
  48. siRNA DNA co-transfection - (reply: 3)
  49. Micro-RNA profiling for differentiating cells - (reply: 1)
  50. microRNA knockdown its target by western blot but can't inhibit the 3'UT - want a reliable protocol to do the 3'UTR luciferase assay (reply: 3)
  51. Problems with amplifying microRNA - (reply: 3)
  52. Complete siRNA newbie!! - Muddling myself up!! (reply: 6)
  53. siRNA and Fugene6 - (reply: 1)
  54. how to check if siRNA is intact - (reply: 1)
  55. siRNA in vivo delivery - (reply: 2)
  56. siRNA newb - How to go about starting an siRNA project (reply: 3)
  57. Weird effect of siRNA on protein in cells - (reply: 3)
  58. siRNA to shRNA...trouble! - (reply: 1)
  59. How to Design a Negative Control siRNA - (reply: 1)
  60. QUESTION-CAPLE OF siRNAs sequences - (reply: 2)
  61. microRNA functional classification - (reply: 1)
  62. Obtaining 3'UTR and perform microRNA target prediction - How to commence? (reply: 2)
  63. siRNA sequence can be used as shRNA? - Stable down regulation of target gene expression. (reply: 1)
  64. Importin alpha siRNA - Looking for siRNA for knockdown of all Importin alpha subtypes (reply: 2)
  65. siRNA overhangs - (reply: 2)
  66. siRNA in the Fridge - (reply: 2)
  67. microRNA profiling using ABI taqman low density microRNA array (TLDA) - (reply: 1)
  68. Double transfection of siRNA - (reply: 2)
  69. Cotransfection of siRNA and plasmid - Cotransfection of siRNA and plasmid (reply: 1)
  70. the necessity of microRNA mutation design - (reply: 1)
  71. siRNA control (scramble seq) causing cell death - what to try next? (reply: 2)
  72. Designing siRNA from Sequence provided by Qiagen - How to design from a given Nucleotide sequence a siRNA?? (reply: 4)
  73. No difference between transfected and untransfected cells - Using eGFP siRNA on GFPHEK293 cells usign flurimeter (reply: 5)
  74. Illumina microRNA expression arrays - Problems with clustering different experiments...HELP! (reply: 5)
  75. siRNA againts Beta Actin Help PLease - (reply: 2)
  76. cell cryopreservation after siRNA transfection - (reply: 1)
  77. The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m - (reply: 8)
  78. microrna quantitation ---use northern blot or qrt-pcr？ - (reply: 1)
  79. Luciferase GL4 siRNA - (reply: 5)
  80. siRNA staining? - (reply: 1)
  81. microRNA database - in both animals and plant (reply: 1)
  82. Choosing the right refrence sample for microRNA array - (reply: 3)
  83. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
  84. siRNA and plasmid cotransfection - (reply: 1)
  85. design parameters for multiple siRNA expression in vector - (reply: 4)
  86. siRNA with low mRNA levels - (reply: 7)
  87. how to test if a given microRNA regulates a gene of interest - microrna target (reply: 7)
  88. How to batch search my microRNA accession numbers? - (reply: 2)
  89. ABI Taqman Low density microRNA Array card - (reply: 3)
  90. novel microRNA prediction - (reply: 2)
  91. siRNA electroporation - (reply: 1)
  92. MCF-7 cells dying after siRNA transfection + estrogen - (reply: 1)
  93. Is it possible to reuse electroporation cuvette to transfect siRNA - (reply: 4)
  94. Silencer® GFP (eGFP) siRNA – AM4626 - (reply: 2)
  95. HepG2 siRNA transfection - (reply: 1)
  96. siRNA - (reply: 2)
  97. in vitro siRNA - wich is the best company for a siRNA for in vitro assay? (reply: 2)
  98. prediction of novel microRNAs from deep sequencing data? - microRNA deep sequencing (reply: 1)
  99. protein complex and siRNA - (reply: 2)
  100. why using siRNA knock down,but get the mRNA products? - (reply: 3)
  101. about the microRNA seqence database - (reply: 3)
  102. siRNA on primary DRG cell - transfection agent/protocol (reply: 3)
  103. Quantify RNAi(siRNA) efficacy - Designing primers for quantification (reply: 2)
  104. siRNA concentration in divinding-growing cells - (reply: 2)
  105. siRNA transfection of RS4-11 and MV-4-11 Cells - (reply: 1)
  106. How to design probes for microRNA Northern Blot - (reply: 4)
  107. siRNA resistant gene - How many bp to change? (reply: 4)
  108. siRNA cell function examples - (reply: 1)
  109. Good SiRNA Gene silencing - (reply: 1)
  110. cellular siRNA localization - (reply: 4)
  111. Define Trasnsfection efficiency interms of siRNA experiment - Sirna uptake Vs transfection efficiency (reply: 12)
  112. Amaxa siRNA - (reply: 3)
  113. Old threads on "siRNA, microRNA and RNAi" are achived here - (reply: 3)
  114. Identification of miRNA and siRNA - (reply: 1)