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in vitro siRNA - wich is the best company for a siRNA for in vitro assay? (Aug/03/2009 )

IŽll buy a siRNA for in vitro assay, and have doubts about the right brand...
Has anyone used siRNA for Akt1? Has it worked out?
thk

-hclbs-

hclbs on Aug 3 2009, 06:44 AM said:

IŽll buy a siRNA for in vitro assay, and have doubts about the right brand...
Has anyone used siRNA for Akt1? Has it worked out?
thk

I had good experience with Dharmacon siRNA oligos (now Thermo Scientific). If you are thinking of shRNA vectors, I had good experience with pSingle inducible vector from Clontech. No experience with Akt1.
Attached File

-cellcounter-

Hi! So you have experience with the pSingle-tTS-shRNA from Clontech?
I am looking for an inducible system to use and I was thinking about this one. Do you think it is a good choice?
But it is really expensive!!! They are asking me about 1500 euros to get it! Is that normal?!
Thanks thanks!
Jojo

-Jojo-