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transfecting siRNA thp-1 after differentiated to macrophage - (Jul/09/2012 )

Do you know a simple way to transfect (siRNA) of thp-1 after differentiated to macrophage?
Lipofectamin did not work.


it must work. what is your protocol?


Hello memari,

When THP1s are differentiated into macrophages, they stop dividing. For Lipofectamine 2000 or Lipofectamine RNAiMAX you need the cells to be dividing in order for the siRNA to be introduced into the cell. You may try some protein-based transfection reagents.