siRNA resuspension in RNase-free water - (Dec/28/2012 )
Hello,
Recently, we ordered Dharmacon ON-TARGET Plus siRNAs in our lab. We have around 5nmol of siRNAs and we want to make a 100uM stock.
I wonder whether we can use RNase-and DNase-free water to make these concentrated stocks (i.e 100uM) instead of 1x siRNA buffer that the company recommends.
Awaiting your valuable suggestions and comments.
Regards,
Kart
-Neanderthal-
Invitrogen's siRNAs come with DEPC treated water for reusupension, so I guess the answer is yes.
-bob1-