siRNA resuspension in RNase-free water - (Dec/28/2012 )
Recently, we ordered Dharmacon ON-TARGET Plus siRNAs in our lab. We have around 5nmol of siRNAs and we want to make a 100uM stock.
I wonder whether we can use RNase-and DNase-free water to make these concentrated stocks (i.e 100uM) instead of 1x siRNA buffer that the company recommends.
Awaiting your valuable suggestions and comments.
Invitrogen's siRNAs come with DEPC treated water for reusupension, so I guess the answer is yes.