Albumin Removal From BSA - (Jan/10/2013 )
I have 250 Bovine serum samples. I would like to remove Albumin from these samples to have the chance to visualize the low abundant Immunoglobulin (IgA and IgM) by FT-IR.
If anyone know protocol to precipitate or remove albumin from these sample just write it to me.
You can use Affinity Chromatography or Ion Exchange Chromatography (Isoelectric Point). If you have less amount maybe with sepharose beads.