Insulin production - (Jan/05/2013 )

What would be the best way to express and purify active insulin?

I don't know how much info you already have, they do this by cloning the two isoforms and transforming into e.coli. Perhaps you can check this animation:

http://www.abpischools.org.uk/page/modules/diabetes/diabetes6.cfm?coSiteNavigation_allTopic=1

If you need more in-depth material you need to check the Diabetes Journal under American Diabetes Association.

Cool source thaks for the hint.