Insulin production - (Jan/05/2013 )
What would be the best way to express and purify active insulin?
I don't know how much info you already have, they do this by cloning the two isoforms and transforming into e.coli. Perhaps you can check this animation:
If you need more in-depth material you need to check the Diabetes Journal under American Diabetes Association.
Cool source thaks for the hint.