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Insulin production - (Jan/05/2013 )

What would be the best way to express and purify active insulin?


I don't know how much info you already have, they do this by cloning the two isoforms and transforming into e.coli. Perhaps you can check this animation:

If you need more in-depth material you need to check the Diabetes Journal under American Diabetes Association.


Cool source thaks for the hint.