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Methylation Related Discussions
  1. Nested BSP Primer Design - (reply: 5)
  2. Setting up bisulfite sequencing in lab - (reply: 7)
  3. Bisulfite/sequencing-based Methylation Analysi - (reply: 14)
  4. fixation and methylation - (reply: 2)
  5. Methylation status analysis of non CpG sites. - (reply: 3)
  6. Preparing overlapping bisufite sequences for methylation analysis - (reply: 2)
  7. AZA treatment induces the genes but bisulfite sequencing does not reveal de-meth - (reply: 11)
  8. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  9. does DNA methylation outside promoter region affect gene Transcription/expressio - (reply: 1)
  10. Determining CpG methylation and effect on gene expression - (reply: 1)
  11. Troubleshooting methylation primers for Bio-Rad PCR - (reply: 3)
  12. Bisulfite Sequencing and PCR Troubleshooting - (reply: 2)
  13. Methylation studies - (reply: 2)
  14. Sigma Imprint DNA methylation quantification assay kit - (reply: 3)
  15. Analysing methylation of plant promoter and enchancer - (reply: 9)
  16. Need Methylation MSP protocol - (reply: 3)
  17. designing a methylation study - (reply: 5)
  18. Understanding methylation for beginners - (reply: 5)
  19. what is the effectors of MSP? - (reply: 3)
  20. Issue with Xba1 and Dam methylation - (reply: 7)
  21. How to create a fully methylated control? - (reply: 4)
  22. Invitrogen Methyl Miner Tips - (reply: 2)
  23. DNA Methylation and genomic variation - (reply: 2)
  24. Primer designing for Methylation - (reply: 6)
  25. Bisulfite PCR and cloning - (reply: 5)
  26. BSP PCR primer design explained - (reply: 11)
  27. Bisulfite sequencing PCR worked - (reply: 5)
  28. Quality of Bisulfite Converted DNA - (reply: 2)
  29. CPG adjuvant - (reply: 1)
  30. How to know if observed methylation is true or a consequence of incomplete or fa - (reply: 2)
  31. Qiagen Epitect Bisulfite Kit - (reply: 3)
  32. Porportion methylation of bisulfite DNA changes with time? - (reply: 4)
  33. BSP testing for differences in methylation pattern by use of melt curve before s - (reply: 1)
  34. Q regarding primer mix for MSP!! - (reply: 4)
  35. suitable DNA extraction and sample freezing for MSP - (reply: 1)
  36. BSP Primers with M13 Tails - (reply: 1)
  37. cytosine methylation stability - (reply: 1)
  38. About Methylated & Non-methylated pUC19 DNA Set - (reply: 1)
  39. Bisulfite PCR Question - (reply: 2)
  40. Bisulfite PCR doesn't work and the primer seems abnormal - (reply: 6)
  41. Bisulfite Primers- "Top" and "Bottom" Strand - (reply: 5)
  42. no DNA fragment pull down in the first CpG island (MBD-seq) - (reply: 2)
  43. designing methylation specific primers - (reply: 3)
  44. Effect of varying DNA concentration levels in methylation analysis - (reply: 2)
  45. Different primer optimalization for nested vs direct MSP? - (reply: 3)
  46. Bisulfite conversion conundrum - (reply: 8)
  47. Help! No bands after BSP! - (reply: 3)
  48. MSP primers- finding the product length - (reply: 4)
  49. Can we use degenerate bases in BSP primers? - (reply: 1)
  50. Can anyone help with my Bisulfite PCR? - (reply: 2)
  51. Plasmid clone in BSP - (reply: 1)
  52. Sequencing of the PCR production in BSP - (reply: 1)
  53. Taq polymerase for MSP - (reply: 1)
  54. RUNX3 Methylation specific PCR not working-please help - (reply: 8)
  55. Spin columns with bisulfite conversion - (reply: 1)
  56. Problem of Direct bisulfite sequencing - (reply: 1)
  57. Bisulfite cloning... reading material - (reply: 1)
  58. DNA in methylation analysis - (reply: 3)
  59. MSP Primers not working - (reply: 6)
  60. plotting DNA methylation profile vs TSS - (reply: 1)
  61. Methylation sensitive enzymes - (reply: 1)
  62. problems with Bisulfite PCR - (reply: 1)
  63. In search of universal and reliable controls for methylation analyses - (reply: 1)
  64. PLATINUM TAQ FOR MSP? - (reply: 2)
  65. Checking my own Methylated Control for MSP - (reply: 1)
  66. Direct sequencing - BSP - (reply: 1)
  67. methylation analysis with MS-HRM (primer design) - (reply: 1)
  68. Methyl Binding Protein-1 (MBD1) / Immunofluorescence - (reply: 2)
  69. DNA methylation of a promoter region in different age groups - (reply: 2)
  70. statistical analysis or quantitative analysis method for bisulfite sequencing r - (reply: 4)
  71. Advice on optimising bisulfite PCR sought - (reply: 2)
  72. checking msp primers - (reply: 2)
  73. "CpG methylation: Blocked by some combinations of overlapping" - (reply: 1)
  74. To isolate DNA, RNA and Protein from same culture flask for methylation study? - (reply: 2)
  75. Bisulfite treatment of DNA follow by Sequencing - (reply: 2)
  76. MSP U primers very difficult to amplify... - (reply: 4)
  77. Direct sequencing PCR product - for bisulfite sequencing (reply: 3)
  78. effect of DNA isolation and storage on methylation - (reply: 1)
  79. which is the best plasmid vector for cloning BSP products? - (reply: 2)
  80. Are my MSP primers good? - MSP primers design by Methyl Primer Express v1.0 (reply: 2)
  81. Measuring methylation on X chromosome of female subjects - How can I overcome artifacts from X linked inactivation in females? (reply: 3)
  82. High throughput methylation mapping - Infinium or other? - (reply: 3)
  83. Methods of Methylation detection not requiring bisulfite treatmetn - (reply: 2)
  84. negative control for msp pcr - (reply: 2)
  85. How to blast methylation specific and unspecific primers - (reply: 1)
  86. Problems designing primers for BSP - (reply: 2)
  87. How is bisulfite converted DNA unstable over time? - (reply: 4)
  88. Putative tumor suppressor promoter heavily methylated yet upregulated by DNA dam - (reply: 3)
  89. problems with MS-HRM - late detection of PCR signal - high resolution melting, methylation (reply: 2)
  90. RNA carrier when using not so good DNA for bisulfite conversion - (reply: 3)
  91. Absence of CpG islands - (reply: 1)
  92. MSP - (reply: 10)
  93. How rapid are DNA methylation changes? - (reply: 1)
  94. Methylation study-the most opted study - (reply: 3)
  95. Drop down during bisulfite sequencing - (reply: 2)
  96. Standardization of HRM for methylation analysis - (reply: 4)
  97. Question: Start with genomic DNA or mRNA for methylation analysis - (reply: 4)
  98. Bisulfite sequencing primer design query (probably simple) - (reply: 4)
  99. Inter assay variation in global methylation Elisa - (reply: 2)
  100. designing MSP primers (dimers NOOOOOOOO!) - (reply: 1)
  101. Are my bisulfite treated sequences methylated or not?? - (reply: 2)
  102. DNA and histone methylation help - (reply: 1)
  103. Can every gene be methylated? - (reply: 1)
  104. Help about BSP question. - (reply: 2)
  105. Amplification of bisulfite converted gDNA for sequencing - (reply: 1)
  106. pcr / primer theory in bisulfite sequencing - (reply: 4)
  107. DNA yields after Bisulfite Treatment - How can I optimize when I have low yield? (reply: 13)
  108. Problem with Qiagen EpiTect Bisulfite Kit - (reply: 2)
  109. Feel really stupid for asking this ...Bisulfite Sequencing, help please! - (reply: 10)
  110. Bisulfite seqencing - Bisulfite seqencing gets unacceptable seqence (reply: 2)
  111. PCR amplification before bisulfite conversion - (reply: 6)
  112. Methylation and luciferase reporter query - (reply: 4)
  113. Bisulfite conversion kits - (reply: 6)
  114. Digestion problem XbaI, methylation sensitive site - (reply: 3)
  115. SNP and DNA methylation - (reply: 1)
  116. Bisulfite sequencing is giving problem - (reply: 7)
  117. methylation specific PCR - Primers - (reply: 1)
  118. Tips for MSP ... - (reply: 3)
  119. Standarization of HRM for methylation - (reply: 4)
  120. Whole Genome Amplification of bisulfite treated DNA - (reply: 7)
  121. no methylation vs full methylation - (reply: 3)
  122. Bisulfite sequencing - (reply: 3)
  123. Finding bisulfite PCR primer sequence - (reply: 3)
  124. No amplification after bisulfite treatment - (reply: 4)
  125. problems when use qPCR to quantify bisulfite modified DNA - (reply: 4)
  126. Primer Design MSP, BSP, MS-HRM - (reply: 3)
  127. Bisulfite sequencing not working! - (reply: 4)
  128. MSP - unwanted amplification - (reply: 5)
  129. Smears in Bisulfite seq. PCR - (reply: 3)
  130. Multiple bands after bisulfite PCR - (reply: 7)
  131. Supposedly etablished MSP not working - E-Cadherin promotor (reply: 9)
  132. tough problems on methylation study - (reply: 4)
  133. How to reduce the bisulfite PCR bias? - (reply: 4)
  134. Regarding methylation and luciferase assay - Regarding methylation and luciferase assay (reply: 4)
  135. Global methylation assay - kit? - (reply: 7)
  136. Help with data from directly sequencing BSP product... - (reply: 2)
  137. BSP primers design, help please - (reply: 2)
  138. Bisulfite treatment - (reply: 7)
  139. Bisulfite sequencing PCR not working - (reply: 5)
  140. Bisulfite Conversion Efficiency - (reply: 1)
  141. Best method for validating DNA methylation array data - (reply: 1)
  142. promoter methylation data analysis - (reply: 3)
  143. bisulfite sequencing cloning problem (methylation of DNa) - bisulfite sequencing cloning problem (methylation of DNa) (reply: 3)
  144. MSP primer troubles - (reply: 3)
  145. Gene expression of methylated gene - (reply: 4)
  146. Measuring global methylation using real time PCR - (reply: 2)
  147. primer for bisulfite sequencing in a known unmethylated region - is it safe? - (reply: 2)
  148. free bisulfite conversion kit - (reply: 2)
  149. are plasmids used for lab work methylated - (reply: 1)
  150. Zymo EZ Methylation Gold DNA bisulphite treatment - No DNA present after treatment (reply: 5)
  151. Methylation analysis in vitro - (reply: 3)
  152. dam methylated DNA in dam - straights - (reply: 1)
  153. How to define CpG islands and what to do if they aren't found? - (reply: 3)
  154. All cytosines are methylated!! - (reply: 4)
  155. Polymerases voor bisulfite sequencing - any fancy new enzymes? (reply: 4)
  156. multiplex methylation analysis (bs conversion based) - (reply: 1)
  157. Methylation specific DMSO PCR - (reply: 1)
  158. Inconsistent MSP results - (reply: 7)
  159. "Quick and Dirty" methylation status estimates - Restriction profiling of DNAnmethylation (reply: 4)
  160. Comparison of Bisulfite conversion kits - (reply: 7)
  161. Site Directed methylation - (reply: 1)
  162. Amount of positive control for bisulfite treatment - (reply: 1)
  163. Bisulfite Sequencing PCR help! - BSP is Failing like Gangbusters. Please help! (reply: 6)
  164. genome methylation analysis/database - analyze all promoters for CpG islands (reply: 1)
  165. Problem cloning bisulfite PCR BSP product - (reply: 6)
  166. DNA methylation analysis - direct sequencing gives CT peaks - (reply: 1)
  167. Whole Genome Methylation Analysis Feasibility - Methylation analysis on DNA from rare cell populations (reply: 3)
  168. Methylation Questions - Some basic questions about methylation (reply: 2)
  169. heredity of DNA methylation - (reply: 2)
  170. global DNA methylation quiagen kit - (reply: 14)
  171. Measuring global methylation by QPCR - (reply: 1)
  172. Manual reverse primer design for bisulfite traeted DNA - (reply: 4)
  173. sequencing clones after BSP : need miniprep ?? - (reply: 1)
  174. DNA methylated PCR - (reply: 2)
  175. Product length for BSP - (reply: 4)
  176. Bisulfite vs metabisulfite - (reply: 3)
  177. BSP PCR for help - no bands of tissue amplification (reply: 3)
  178. Methylation specific PCR - Methylation specific PCR - problem with controls.... (reply: 2)
  179. weak bands for methylated DNA - (reply: 4)
  180. How to present bisulfite seq data - with CpGviewer (reply: 7)
  181. BS PCR..please help - DNA methylation analysis (reply: 15)
  182. BSP PCR standardization...help - (reply: 8)
  183. DNA Enrichment for Methylation Analysis - (reply: 8)
  184. Perplexing Bisulfite Sequencing Problem - (reply: 3)
  185. Help me! How to design nested BSP primers? - (reply: 4)
  186. length of bisulfite sequencing reads on a ABI - my reads are not so long!!! (reply: 2)
  187. CpG Complementary Symmetry - (reply: 2)
  188. Strange results from bisulfite sequencing - (reply: 2)
  189. Integrity of treated DNA - Is quality of bisulfite treated DNA poor? (reply: 1)
  190. 5-AZA, Reversing DNA methylation (epigenetic reactivation experiments) - (reply: 3)
  191. Sybre green taq man PCR assay for quantitative methylation detection - (reply: 2)
  192. Imcomplete bisulfite treatment - (reply: 2)
  193. Bisulfite Sequencing PCR - (reply: 4)
  194. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
  195. Is gDNA digestion before bisulfite conversion important? - (reply: 5)
  196. Bisulfite PCR Inconsistent, often smears - (reply: 4)
  197. Direct sequencing of BSP product - (reply: 3)
  198. Preparing Methyl Cellulose - (reply: 4)
  199. BSP primers - (reply: 2)
  200. Anybody knows a good histone methylation inhibitor? - (reply: 2)
  201. MSP Score - (reply: 1)
  202. DNA methylation prediction in plant DNA - (reply: 2)
  203. methylation erased by pcr: how and why - why does PCR erase methylation information? (reply: 7)
  204. Questions regarding bisulfite treatment of DNA - (reply: 2)
  205. Bisulfite genomic sequencing PCR - PCR conditions (reply: 7)
  206. comparison of bisulfite sequencing and base specific cleavage coupled with Mass - (reply: 5)
  207. How stable is methylated DNA? - (reply: 4)
  208. Suitable sample for promoter methylation screening - (reply: 4)
  209. Analysis of direct bisulfite sequencing data - (reply: 9)
  210. MethylCode Bisulfite Conversion Kit from Invitrogen - Used this kit? (reply: 4)
  211. EZ DNA Methylation kit - (reply: 1)
  212. polymerase for BSP - (reply: 2)
  213. Designing and analyzing BSP - (reply: 1)
  214. BSP,MSP primer design? - (reply: 1)
  215. BISULFITE CONVERTION ?? TROUBLE SHOOTING - (reply: 4)
  216. MSP: No PCR product for one cell line but yes for the other cell lines - (reply: 3)
  217. GAPDH methylated or not - (reply: 2)
  218. How adjacent or remote CpG affects expression? - (reply: 5)
  219. positive control for BSP? - (reply: 12)
  220. Heterogeneous methylation in a same cell line - (reply: 1)
  221. Confirm DNA enrichment of methylation array - (reply: 2)
  222. modifications in bisulfite treatment - (reply: 6)
  223. MSP problem - (reply: 7)
  224. western for methylated histone - (reply: 7)
  225. How to design prime detecting plant methylation? - (reply: 3)
  226. Negative control problem in BSP - (reply: 1)
  227. positive control for MSP - (reply: 6)
  228. Generating primers for MSP - (reply: 1)
  229. Zymo CpG Methylase (M.SssI) Kit - Alternative Distributor to NEB (reply: 1)
  230. MSP SENSITIVITY - (reply: 1)
  231. methylation by MSSS1(NEB )for reporter gene analysis - (reply: 2)
  232. Does phenol extraction have effect on DNA methylation? - (reply: 4)
  233. Effect of freezing on DNA methylation? - (reply: 6)