Protocol Online logo
Top : New Forum Archives (2009-): : DNA Methylation and Epigenetics

Zymo CpG Methylase (M.SssI) Kit - Alternative Distributor to NEB (Feb/11/2009 )

Hi All!

Has anyone recently tested the new Zymo CpG Methylase Kit?

CpG Methylase (M.SssI)

They seem to have another buffer and definately a different incubation temperature. Any experiences regarding complete methylation?

Best,

MoB

-MoB-

MoB on Feb 11 2009, 01:01 PM said:

Hi All!

Has anyone recently tested the new Zymo CpG Methylase Kit?

CpG Methylase (M.SssI)

They seem to have another buffer and definately a different incubation temperature. Any experiences regarding complete methylation?

Best,

MoB


Hi All!

I give feedback on my own posting. I tested the Zymo CpG Methylase Kit after convincing my lab-leader that it should be worth to try. We had some problems receiving a complete methylation with the NEB M.SssI kit. With the Zymo kit methylation seems to be complete even for extremely CpG-rich regions for which the NEB kit had some difficulties. In all cases we weren't able to detect any residual unmethylated DNA.

The main differences are a different buffer and overnight-incubation at 30C (NEB 37C, 1-4h). Zymo ships their kit on dry-ice. Shipment by NEB is done on cool-packs. This seems to have a high impact on the stability especially of the SAM. This is the component we have identified to be most critical. Any comments on that?

Best,

MoB

-MoB-