Problem with Qiagen EpiTect Bisulfite Kit - (Oct/26/2010 )

Hi!
I am using Qiagen EpiTect Bisulfite Kit for detect methylation status.
I isolated DNA, quantified it, and I did PCR assay.
I have only amplification in PCR with primers for DNA methylated.
I have not amplification in PCR with primers for DNA no methylated.
I think the problem is in bisulfite treatment.
Please!!!
Help!!!
Where is the problem???

are you expecting your samples to be unmethylated or methylated? does sound like your conversion has worked.

Nick

methylnick on Tue Oct 26 19:47:52 2010 said: