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fixation and methylation - (Apr/06/2014 )

I am planning on extracting DNA from tissues for a methylation study.  Would a prior methanol fixation affect the DNA extraction or methylation?  Thanks


I doubt if this will be a problem.


Nope. But keep in mind, since DNA from paraffin blocks is already degraded, and will be further fragmented during bisulfite conversion, amplicons size should be kept to a minimum when designing primers.