fixation and methylation - (Apr/06/2014 )
I am planning on extracting DNA from tissues for a methylation study. Would a prior methanol fixation affect the DNA extraction or methylation? Thanks
I doubt if this will be a problem.
Nope. But keep in mind, since DNA from paraffin blocks is already degraded, and will be further fragmented during bisulfite conversion, amplicons size should be kept to a minimum when designing primers.