free bisulfite conversion kit - (Mar/05/2010 )
hey guys, saw this in Nature Methods:
www.epigentek.com/bisulflash
too bad im already finishing up on my methylation studies but it may be of interest to some of you
cheers
Okay, 10 reactions for free...
But this website borders on the impertinent. I think everybody knows who's behind 'Supplier Z' and 'Supplier Q'. And their benchmarks can only be reached in their dreams. I've recently tested and compared all available bisulfite-conversion kits and only two kits turned out to give high-conversion rates, high yields, high purity of the eluate and a low DNA-degradation. And these were the kits from 'Supplier Z' and Supplier Q'. Just to mention it: the bottom quality was reached by the Epigentek kit. Save your precious samples and don't use this kit!
MoB on Mar 6 2010, 06:45 AM said:
But this website borders on the impertinent. I think everybody knows who's behind 'Supplier Z' and 'Supplier Q'. And their benchmarks can only be reached in their dreams. I've recently tested and compared all available bisulfite-conversion kits and only two kits turned out to give high-conversion rates, high yields, high purity of the eluate and a low DNA-degradation. And these were the kits from 'Supplier Z' and Supplier Q'. Just to mention it: the bottom quality was reached by the Epigentek kit. Save your precious samples and don't use this kit!
Really? i don't know, I've had some pretty good experiences with an Epigentek kit (not for bisulfite conversion though). I started with Sigma's bisulfite and global DNA methylation quantitation kits until someone in these forums mentioned they were just re-badged Epigentek kits.
maybe I'm wrong -- but thanks for the info!