Site Directed methylation - (Dec/17/2009 )

Hello friends,
I am new to the area of epigenetics and am planning some experiments related to the same. I am wondering if there are any methods similar to site-directed mutagenesis, so as to perform site directed methylation. Any information is appreciated.

Regards to all

Sam

Hi Sam,

we have been grappling with this problem also in the past,

have a look at NEB for Methyltransferases that block restriction sites ie: they recognise a particular restriction site and methylates it.

There are also non-specific methyltransferases including CpG methylase (SssI) and a GpC methylase too I recently found out.

Otherwise there are oligo synthesis companies that could generate an oligo for you with a methylated CpG site.

Good luck

Nick