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Membrane Related Discussions
  1. Outer membrane protein extraction - (reply: 5)
  2. Express two different membrane proteins on the same promoter - (reply: 1)
  3. Storing membrane after antibody incubation - (reply: 2)
  4. Can I incubate two membranes in one container? - (reply: 2)
  5. Human cell membrane protein Western blot! - (reply: 2)
  6. Can propidium iodide be used on WBC nuclei with cell membrane removed - (reply: 1)
  7. Blocking and Washing membrane - (reply: 3)
  8. Does ultrasonic probe sonication break up mitochondria or nuclear membrane? - (reply: 2)
  9. Does TritonX-100 lysis buffer break up mitochondrial membrane? - (reply: 2)
  10. Does Ultrasonic probe sonication break either mitochondrial or nucler membrane? - (reply: 3)
  11. I stored my PVDF membrane at 4C ? - (reply: 2)
  12. Is it possible to "over-block" a membrane? - (reply: 5)
  13. storing membrane - (reply: 2)
  14. Methods to quantify human GLUT4 translocation to plamsa membrane - (reply: 1)
  15. Choosing MWCO For Dialysis Membrane - (reply: 3)
  16. fluorescent tag on membrane protein - (reply: 2)
  17. membrane protein expression - (reply: 2)
  18. Incomplete Blotting and staining of NC membrane - (reply: 7)
  19. genomic DNA extraction on silica-based membrane - (reply: 1)
  20. Breaking nuclear membrane with needle is sufficient (no sonicator nor dounce)? - (reply: 2)
  21. What is the membrane made of in DNA purification kits - (reply: 3)
  22. Problems with western membrane stripping - (reply: 2)
  23. PVDF membrane long-term storage after transfer - (reply: 3)
  24. LMW proteins not transfer of gel to membrane - (reply: 1)
  25. Biotinylated RNA on Nylon Membrane - (reply: 2)
  26. Membrane protein - (reply: 1)
  27. Undergrad "activated" a NS membrane with 100% MeOH... Any hope? - (reply: 5)
  28. Mix of Milk, BSA, Casein for membrane blocking - (reply: 3)
  29. Membrane protein extraction - (reply: 2)
  30. Membrane Protein Extraction - (reply: 2)
  31. membrane proteomics - (reply: 1)
  32. Membrane proteins on 2D gel electrophoresis..... - (reply: 1)
  33. Membrane vs. Cytosol Fractionation - (reply: 2)
  34. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
  35. Ponceau red problem: membrane turned red - (reply: 3)
  36. Principle of silica membrane binding of nucleic acid - (reply: 6)
  37. membrane proteins ultrafiltration - (reply: 2)
  38. Membrane protein concentration - (reply: 1)
  39. Protein concentration of a labelled membrane protein - (reply: 2)
  40. The pitfalls of Membrane Preps - (reply: 1)
  41. Membrane Protein SDS PAGE and WB - (reply: 1)
  42. SDS solubilisation, Membrane Proteins and Micelle Formation - (reply: 2)
  43. Immuno-staining for cell surface/membrane markers after permeabilisation - (reply: 4)
  44. Stripping the membrane for LiCor - (reply: 1)
  45. Mass-spectrometry of membrane proteins - (reply: 1)
  46. Western blot membrane imaging - (reply: 7)
  47. Storage of Membrane before addition of primary antibody - (reply: 3)
  48. mass spectrometry and tryptic digestion on membrane proteins - (reply: 5)
  49. PVDF membrane silver staining protocol - (reply: 5)
  50. Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)
  51. Reprobing a PVDF membrane which has gone dry - (reply: 1)
  52. Secondary Ab is M.I.A. ... how long can I leave my membrane in wash buffer? - (reply: 1)
  53. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
  54. problems with housekeeping antibodies when re-probing membranes - (reply: 3)
  55. How to store a membrane? - (reply: 1)
  56. membrane protein expression - (reply: 1)
  57. RNA transfer to nitrocellulose membrane (Northern blot) - (reply: 2)
  58. 2-D electrophoresis of sperm membrane extracts - (reply: 9)
  59. Smeared transfer on nitrocellulose membrane - (reply: 2)
  60. Help on using different antibodies in the same membrane - (reply: 8)
  61. Stripping buffer for nitrocellulose membrane - (reply: 1)
  62. BN-PAGE of purified membrane associated protein - (reply: 5)
  63. Re-using membranes with ghost bands - (reply: 2)
  64. Dot Blot - Protein not adhering to membrane - (reply: 1)
  65. Membrane proteins - (reply: 6)
  66. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
  67. Areas of membrane bleached - (reply: 1)
  68. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
  69. membrane filters for chromatography buffers - (reply: 1)
  70. ELISA plate coating with plasma membrane vesicles - (reply: 1)
  71. HELP!!! Non-specific binding on my membrane - (reply: 7)
  72. Help! Half membrane exposed perfectly. Half membrane is cloudy - (reply: 2)
  73. Imaging western blot membrane - (reply: 2)
  74. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
  75. Membrane solubilisation - (reply: 2)
  76. protocol to transfer protein on transfer membrane fluoride bind - please tell me how to transfer proteins from sds page (reply: 4)
  77. What causes RNA/DNA to unbind from silica membrane? - (reply: 1)
  78. Nitrocellulose membrane storage after transfer - (reply: 6)
  79. HELP!?!? with MEMBRANE PROTEIN extraction - Extraction of GPCRs from HEK293T for IB and IP (reply: 1)
  80. Identification of Membrane Receptor protein, how ?? - I wanna use MS approach ! (reply: 4)
  81. Membrane fluidity by flow cytometry - (reply: 3)
  82. Having trouble with stripping PVDF membrane during WesterDot blot - (reply: 1)
  83. PVDF membrane in direct contact with kodak film - PVDF membrane accidentally touching kodak membrane (reply: 1)
  84. Filming membrane (Western Blot & ECL) - (reply: 1)
  85. Homogenization for Plasma Membrane Protein Extraction - (reply: 1)
  86. Molecular Cell Biology - Trafficking and function of a membrane enzyme (reply: 1)
  87. Dry or not PVDF membrane after transfer - (reply: 2)
  88. Western Blots on Membrane Proteins - (reply: 9)
  89. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
  90. kit fpr cell membrane preparation? - (reply: 2)
  91. total cell protein vs membrane protein - (reply: 4)
  92. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
  93. PVDF membrane dried after transfer (Western) - (reply: 6)
  94. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
  95. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
  96. How to store wet dialysis membranes once opened? - (reply: 4)
  97. Cellulose Membranes - Problem with my membrane (reply: 3)
  98. Re-use membrane in Western Blot - (reply: 3)
  99. NP40 or Triton as membrane protein detergent - (reply: 2)
  100. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
  101. Brown PVDF membrane after rehydration - (reply: 5)
  102. membrane protein aggregation - (reply: 1)
  103. problem of membrane protein FPLC - (reply: 1)
  104. my western blots of membrane protein do not work - (reply: 9)
  105. Membrane Solubilize with CHAPSO - Insoluble fraction (reply: 1)
  106. buffer for membrane protein - (reply: 1)
  107. membrane protein purification - (reply: 6)
  108. membrane type of cell culture filtration - (reply: 3)
  109. membrane damaged = cell death? - (reply: 2)
  110. Bands visible in membrane but not in the film - ?? (reply: 10)
  111. Permeabilize Eukaryotic Membranes - (reply: 11)
  112. MEthanol in NC membrane - (reply: 7)
  113. reconstitution of membrane proteins into liposomes - (reply: 1)
  114. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
  115. Membrane protein isolation - (reply: 1)
  116. membrane protein extraction buffer - (reply: 1)
  117. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
  118. I need help with the membrane fatty acid determination - (reply: 9)
  119. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
  120. Problem transferring HIV-1 tat protein to a PVDF membrane for western blot - (reply: 5)
  121. Mitochondrial membrane potential gain - (reply: 1)
  122. Mitochondrial membrane potential - (reply: 3)
  123. Outer membrane preparation protocol. - (reply: 1)
  124. my membranes are blue! - silly mistake, what to do? (reply: 1)
  125. isolation of membrane associated proteins - (reply: 2)
  126. Beginner Question: Membrane position during Blocking and washing? - (reply: 1)
  127. How to store membrane with primary antibody? - (reply: 5)
  128. taking protein out of membrane - (reply: 1)
  129. Big white spots on PVDF membrane - (reply: 1)
  130. nylon membranes bleaching - (reply: 2)
  131. Cutting membrane after transfer - (reply: 27)
  132. Effect of sonication on membrane anchored proteins - (reply: 1)
  133. resuspend membrane pellet - (reply: 2)
  134. resuspend membrane pellet - (reply: 3)
  135. HELP URGENT! FLUID MOSAIC / CELL MEMBRANE - (reply: 2)
  136. Detergent concentration for nuclear membrane dissolving - (reply: 2)