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Membrane Related Discussions
  1. Blocking and Washing membrane - (reply: 3)
  2. Does ultrasonic probe sonication break up mitochondria or nuclear membrane? - (reply: 2)
  3. Does TritonX-100 lysis buffer break up mitochondrial membrane? - (reply: 2)
  4. Does Ultrasonic probe sonication break either mitochondrial or nucler membrane? - (reply: 3)
  5. I stored my PVDF membrane at 4C ? - (reply: 2)
  6. Is it possible to "over-block" a membrane? - (reply: 5)
  7. storing membrane - (reply: 2)
  8. Methods to quantify human GLUT4 translocation to plamsa membrane - (reply: 1)
  9. Choosing MWCO For Dialysis Membrane - (reply: 3)
  10. fluorescent tag on membrane protein - (reply: 2)
  11. membrane protein expression - (reply: 2)
  12. Incomplete Blotting and staining of NC membrane - (reply: 7)
  13. genomic DNA extraction on silica-based membrane - (reply: 1)
  14. Breaking nuclear membrane with needle is sufficient (no sonicator nor dounce)? - (reply: 2)
  15. What is the membrane made of in DNA purification kits - (reply: 3)
  16. Problems with western membrane stripping - (reply: 2)
  17. PVDF membrane long-term storage after transfer - (reply: 3)
  18. LMW proteins not transfer of gel to membrane - (reply: 1)
  19. Biotinylated RNA on Nylon Membrane - (reply: 2)
  20. Membrane protein - (reply: 1)
  21. Undergrad "activated" a NS membrane with 100% MeOH... Any hope? - (reply: 5)
  22. Mix of Milk, BSA, Casein for membrane blocking - (reply: 3)
  23. Membrane protein extraction - (reply: 2)
  24. Membrane Protein Extraction - (reply: 2)
  25. membrane proteomics - (reply: 1)
  26. Membrane proteins on 2D gel electrophoresis..... - (reply: 1)
  27. Membrane vs. Cytosol Fractionation - (reply: 2)
  28. Stain nitrocellulose membrane with coomassie stain? - (reply: 3)
  29. Ponceau red problem: membrane turned red - (reply: 3)
  30. Principle of silica membrane binding of nucleic acid - (reply: 6)
  31. membrane proteins ultrafiltration - (reply: 2)
  32. Membrane protein concentration - (reply: 1)
  33. Protein concentration of a labelled membrane protein - (reply: 2)
  34. The pitfalls of Membrane Preps - (reply: 1)
  35. Membrane Protein SDS PAGE and WB - (reply: 1)
  36. SDS solubilisation, Membrane Proteins and Micelle Formation - (reply: 2)
  37. Immuno-staining for cell surface/membrane markers after permeabilisation - (reply: 4)
  38. Stripping the membrane for LiCor - (reply: 1)
  39. Mass-spectrometry of membrane proteins - (reply: 1)
  40. Western blot membrane imaging - (reply: 7)
  41. Storage of Membrane before addition of primary antibody - (reply: 3)
  42. mass spectrometry and tryptic digestion on membrane proteins - (reply: 5)
  43. PVDF membrane silver staining protocol - (reply: 5)
  44. Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)
  45. Reprobing a PVDF membrane which has gone dry - (reply: 1)
  46. Secondary Ab is M.I.A. ... how long can I leave my membrane in wash buffer? - (reply: 1)
  47. Ponceau on PVDF membranes affecting immunoprecipitation antibody staining?? - (reply: 3)
  48. problems with housekeeping antibodies when re-probing membranes - (reply: 3)
  49. How to store a membrane? - (reply: 1)
  50. membrane protein expression - (reply: 1)
  51. RNA transfer to nitrocellulose membrane (Northern blot) - (reply: 2)
  52. 2-D electrophoresis of sperm membrane extracts - (reply: 9)
  53. Smeared transfer on nitrocellulose membrane - (reply: 2)
  54. Help on using different antibodies in the same membrane - (reply: 8)
  55. Stripping buffer for nitrocellulose membrane - (reply: 1)
  56. BN-PAGE of purified membrane associated protein - (reply: 5)
  57. Re-using membranes with ghost bands - (reply: 2)
  58. Dot Blot - Protein not adhering to membrane - (reply: 1)
  59. Membrane proteins - (reply: 6)
  60. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
  61. Areas of membrane bleached - (reply: 1)
  62. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
  63. membrane filters for chromatography buffers - (reply: 1)
  64. ELISA plate coating with plasma membrane vesicles - (reply: 1)
  65. HELP!!! Non-specific binding on my membrane - (reply: 7)
  66. Help! Half membrane exposed perfectly. Half membrane is cloudy - (reply: 2)
  67. Imaging western blot membrane - (reply: 2)
  68. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
  69. Membrane solubilisation - (reply: 2)
  70. protocol to transfer protein on transfer membrane fluoride bind - please tell me how to transfer proteins from sds page (reply: 4)
  71. What causes RNA/DNA to unbind from silica membrane? - (reply: 1)
  72. Nitrocellulose membrane storage after transfer - (reply: 6)
  73. HELP!?!? with MEMBRANE PROTEIN extraction - Extraction of GPCRs from HEK293T for IB and IP (reply: 1)
  74. Identification of Membrane Receptor protein, how ?? - I wanna use MS approach ! (reply: 4)
  75. Membrane fluidity by flow cytometry - (reply: 3)
  76. Having trouble with stripping PVDF membrane during WesterDot blot - (reply: 1)
  77. PVDF membrane in direct contact with kodak film - PVDF membrane accidentally touching kodak membrane (reply: 1)
  78. Filming membrane (Western Blot & ECL) - (reply: 1)
  79. Homogenization for Plasma Membrane Protein Extraction - (reply: 1)
  80. Molecular Cell Biology - Trafficking and function of a membrane enzyme (reply: 1)
  81. Dry or not PVDF membrane after transfer - (reply: 2)
  82. Western Blots on Membrane Proteins - (reply: 9)
  83. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
  84. kit fpr cell membrane preparation? - (reply: 2)
  85. total cell protein vs membrane protein - (reply: 4)
  86. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
  87. PVDF membrane dried after transfer (Western) - (reply: 6)
  88. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
  89. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
  90. How to store wet dialysis membranes once opened? - (reply: 4)
  91. Cellulose Membranes - Problem with my membrane (reply: 3)
  92. Re-use membrane in Western Blot - (reply: 3)
  93. NP40 or Triton as membrane protein detergent - (reply: 1)
  94. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
  95. Brown PVDF membrane after rehydration - (reply: 5)
  96. membrane protein aggregation - (reply: 1)
  97. problem of membrane protein FPLC - (reply: 1)
  98. my western blots of membrane protein do not work - (reply: 9)
  99. Membrane Solubilize with CHAPSO - Insoluble fraction (reply: 1)
  100. buffer for membrane protein - (reply: 1)
  101. membrane protein purification - (reply: 6)
  102. membrane type of cell culture filtration - (reply: 3)
  103. membrane damaged = cell death? - (reply: 2)
  104. Bands visible in membrane but not in the film - ?? (reply: 10)
  105. Permeabilize Eukaryotic Membranes - (reply: 11)
  106. MEthanol in NC membrane - (reply: 7)
  107. reconstitution of membrane proteins into liposomes - (reply: 1)
  108. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
  109. Membrane protein isolation - (reply: 1)
  110. membrane protein extraction buffer - (reply: 1)
  111. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
  112. I need help with the membrane fatty acid determination - (reply: 9)
  113. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
  114. Problem transferring HIV-1 tat protein to a PVDF membrane for western blot - (reply: 5)
  115. Mitochondrial membrane potential gain - (reply: 1)
  116. Mitochondrial membrane potential - (reply: 3)
  117. Outer membrane preparation protocol. - (reply: 1)
  118. my membranes are blue! - silly mistake, what to do? (reply: 1)
  119. isolation of membrane associated proteins - (reply: 2)
  120. Beginner Question: Membrane position during Blocking and washing? - (reply: 1)
  121. How to store membrane with primary antibody? - (reply: 5)
  122. taking protein out of membrane - (reply: 1)
  123. Big white spots on PVDF membrane - (reply: 1)
  124. nylon membranes bleaching - (reply: 2)
  125. Cutting membrane after transfer - (reply: 27)
  126. Effect of sonication on membrane anchored proteins - (reply: 1)
  127. resuspend membrane pellet - (reply: 2)
  128. resuspend membrane pellet - (reply: 3)
  129. HELP URGENT! FLUID MOSAIC / CELL MEMBRANE - (reply: 2)
  130. Detergent concentration for nuclear membrane dissolving - (reply: 2)