Protocol Online logo
Top : New Forum Archives (2009-): : Indexed by Topic
Membrane Related Discussions
  1. problems with housekeeping antibodies when re-probing membranes - (reply: 3)
  2. How to store a membrane? - (reply: 1)
  3. membrane protein expression - (reply: 1)
  4. RNA transfer to nitrocellulose membrane (Northern blot) - (reply: 2)
  5. 2-D electrophoresis of sperm membrane extracts - (reply: 9)
  6. Smeared transfer on nitrocellulose membrane - (reply: 2)
  7. Help on using different antibodies in the same membrane - (reply: 8)
  8. Stripping buffer for nitrocellulose membrane - (reply: 1)
  9. BN-PAGE of purified membrane associated protein - (reply: 5)
  10. Re-using membranes with ghost bands - (reply: 2)
  11. Dot Blot - Protein not adhering to membrane - (reply: 1)
  12. Membrane proteins - (reply: 6)
  13. Transfer not occurring on right hand side of membrane correctly - (reply: 3)
  14. Areas of membrane bleached - (reply: 1)
  15. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
  16. membrane filters for chromatography buffers - (reply: 1)
  17. ELISA plate coating with plasma membrane vesicles - (reply: 1)
  18. HELP!!! Non-specific binding on my membrane - (reply: 7)
  19. Help! Half membrane exposed perfectly. Half membrane is cloudy - (reply: 2)
  20. Imaging western blot membrane - (reply: 2)
  21. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
  22. Membrane solubilisation - (reply: 2)
  23. protocol to transfer protein on transfer membrane fluoride bind - please tell me how to transfer proteins from sds page (reply: 4)
  24. What causes RNA/DNA to unbind from silica membrane? - (reply: 1)
  25. Nitrocellulose membrane storage after transfer - (reply: 6)
  26. HELP!?!? with MEMBRANE PROTEIN extraction - Extraction of GPCRs from HEK293T for IB and IP (reply: 1)
  27. Identification of Membrane Receptor protein, how ?? - I wanna use MS approach ! (reply: 4)
  28. Membrane fluidity by flow cytometry - (reply: 3)
  29. Having trouble with stripping PVDF membrane during WesterDot blot - (reply: 1)
  30. PVDF membrane in direct contact with kodak film - PVDF membrane accidentally touching kodak membrane (reply: 1)
  31. Filming membrane (Western Blot & ECL) - (reply: 1)
  32. Homogenization for Plasma Membrane Protein Extraction - (reply: 1)
  33. Molecular Cell Biology - Trafficking and function of a membrane enzyme (reply: 1)
  34. Dry or not PVDF membrane after transfer - (reply: 2)
  35. Western Blots on Membrane Proteins - (reply: 9)
  36. Cloning strategy for a membrane protein - did I do anything wrongly? (reply: 2)
  37. kit fpr cell membrane preparation? - (reply: 2)
  38. total cell protein vs membrane protein - (reply: 4)
  39. No signal on dried PVDF membranes after MeOH rehydration/stripping/reprobing - (reply: 3)
  40. PVDF membrane dried after transfer (Western) - (reply: 6)
  41. Which side of the membrane should face up during incubation steps? - Does it matter? (reply: 4)
  42. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
  43. How to store wet dialysis membranes once opened? - (reply: 4)
  44. Cellulose Membranes - Problem with my membrane (reply: 3)
  45. Re-use membrane in Western Blot - (reply: 3)
  46. NP40 or Triton as membrane protein detergent - (reply: 1)
  47. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
  48. Brown PVDF membrane after rehydration - (reply: 5)
  49. membrane protein aggregation - (reply: 1)
  50. problem of membrane protein FPLC - (reply: 1)
  51. my western blots of membrane protein do not work - (reply: 9)
  52. Membrane Solubilize with CHAPSO - Insoluble fraction (reply: 1)
  53. buffer for membrane protein - (reply: 1)
  54. membrane protein purification - (reply: 6)
  55. membrane type of cell culture filtration - (reply: 3)
  56. membrane damaged = cell death? - (reply: 2)
  57. Bands visible in membrane but not in the film - ?? (reply: 10)
  58. Permeabilize Eukaryotic Membranes - (reply: 11)
  59. MEthanol in NC membrane - (reply: 7)
  60. reconstitution of membrane proteins into liposomes - (reply: 1)
  61. Membrane dried after secondary antibody - can I still develop color? (reply: 6)
  62. Membrane protein isolation - (reply: 1)
  63. membrane protein extraction buffer - (reply: 1)
  64. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
  65. I need help with the membrane fatty acid determination - (reply: 9)
  66. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
  67. Problem transferring HIV-1 tat protein to a PVDF membrane for western blot - (reply: 5)
  68. Mitochondrial membrane potential gain - (reply: 1)
  69. Mitochondrial membrane potential - (reply: 3)
  70. Outer membrane preparation protocol. - (reply: 1)
  71. my membranes are blue! - silly mistake, what to do? (reply: 1)
  72. isolation of membrane associated proteins - (reply: 2)
  73. Beginner Question: Membrane position during Blocking and washing? - (reply: 1)
  74. How to store membrane with primary antibody? - (reply: 5)
  75. taking protein out of membrane - (reply: 1)
  76. Big white spots on PVDF membrane - (reply: 1)
  77. nylon membranes bleaching - (reply: 2)
  78. Cutting membrane after transfer - (reply: 26)
  79. Effect of sonication on membrane anchored proteins - (reply: 1)
  80. resuspend membrane pellet - (reply: 2)
  81. resuspend membrane pellet - (reply: 3)
  82. HELP URGENT! FLUID MOSAIC / CELL MEMBRANE - (reply: 2)
  83. Detergent concentration for nuclear membrane dissolving - (reply: 2)