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Membrane Solubilize with CHAPSO - Insoluble fraction (Feb/23/2010 )

Hi everyone,

In my lab we solubilize membrane with 1% CHAPSO and then use the centrifuge to separate out the insoluble from the soluble fractions. What would I expect to find in the insoluble fraction and how do I study this?

Thank you


membrane fragments, insoluble proteins and nuclei (and maybe some other organelles).