resuspend membrane pellet - (Mar/20/2009 )

Hi everyone,

I am working with a membrane protein. Fractionation involves a centrifugation step at 100 000 g for 1 hour. However, the pellet after centrifugation is so resilient to resuspension.

Can anyone suggest a good way to resuspend a membrane protein pellet after high speed spin?
Thanks inadvance

GE

-genz-

you could try a dounce homogenizer.

-mdfenko-

Hi

Use mild denaturing agents Like Urea or GuCl in less conecntrations to solubilize the preoteins. Dialyse if possible

Regards
Sudhakar

genz on Mar 19 2009, 10:28 PM said:

-sudhakar mutyala-