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resuspend membrane pellet - (Mar/19/2009 )

Hi everyone,

I am working with a membrane protein. Fractionation involves a centrifugation step at 100 000 g for 1 hour. However, the pellet after centrifugation is so resilient to resuspension.

Can anyone suggest a good way to resuspend a membrane protein pellet after high speed spin?
Thanks inadvance

GE

-genz-

you could try a dounce homogenizer.

-mdfenko-

Hi

Use mild denaturing agents Like Urea or GuCl in less conecntrations to solubilize the preoteins. Dialyse if possible


Regards
Sudhakar

genz on Mar 19 2009, 10:28 PM said:

Hi everyone,

I am working with a membrane protein. Fractionation involves a centrifugation step at 100 000 g for 1 hour. However, the pellet after centrifugation is so resilient to resuspension.

Can anyone suggest a good way to resuspend a membrane protein pellet after high speed spin?
Thanks inadvance

GE

-sudhakar mutyala-