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subcellular fractionation problem - (Mar/21/2009 )

Dear all
I have used PIERCE kit for subcellular fractionation to prepare nuclear and cytosolic extracts. Although I followed the kit instructions but the fractions were not clean as I found beta actin in both fractions. Does anyone knows a better kit for preparation of cytosolic, nuclear and mitochondrial fractions?
one more thing, can I use syringe for homogenization instaed of vortexing?


I was told that beta actin can be sometimes found in mitochondria (amazingly....)
And tubulin might be a better control instead

-Sharon Pek-