Protocol Online logo
Top : New Forum Archives (2009-): : Protein Expression and Purification

Where to obtain strain E. coli K12 SB536 ? - (Mar/18/2015 )

Dear all,


does anyone know where to get the E. coli K-12 SB536 strain (good for expression of proteins in periplasm)? I searched the Google all over, however I did not get any useful clues.




try looking in this article:


Thank you, but there is no mention of this strain. Actually, the primary citation is, however this (slightly old) paper is from people at Genentech.

It seems that this strain is not commercially available. Perhaps it can be obtained from some group?


I would try the Yale E. coli Genetic Stock Center.