Met trouble in ligation! - insert:sacII,XhoI;vector:sacII,SalI (Mar/30/2002 )
insert:sacII,XhoI;vector:sacII,SalI, is there any good method to improve this ligation ? XhoI and SalI have the same 5' terminal after digestion.
XhoI and SalI are blunted with T4 DNA polymerase