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Ligation with PLAFR - (Jun/09/2004 )

Please help
I am trying to ligate fragments of DNA that have been double digested with Bgl II and Hind III into the vector PLAFR3.
I am making my cells competent by washing in Calcium chloride and using freeze thaw to transform them.
can anyone offer sugesstions of why this is not working mad.gif


Hi alshia,

Many things in your experiment could have gone wrong, such as ligation, preparation of competent cells, and transformation. Before others could answer your question, please provide some details:

What type of your DNA is, PCR product or DNA cut from from a vector?
How did you prepare your vector?